Hormone-sensitive lipase in Japanese flounder Paralichthys olivaceus: the potential function of the inclinator muscle of fin as a lipid storage site

Abstract

Hormone-sensitive lipase (HSL) is an important regulator of lipolysis in mammals, but little is known about its function in other animals. For a better understanding of fish HSL and potential application of the HSL-mediated lipolysis in aquaculture, we cloned HSL cDNAs from Japanese flounder Paralichthys olivaceus. The full-length cDNAs of two HSL genes, designated HSL1 and HSL2, consisted of 2,922 and 2,832 bp, respectively. RT-PCR showed that their transcripts were abundant in the inclinator muscle of fin, liver, and skeletal muscle. Quantitative real-time PCR further revealed that the transcripts of HSL1 and HSL2 genes were broadly expressed in all tissues tested, with the highest abundance in the inclinator muscle of fin. The inclinator muscle of fin also contained many adipocytes. In addition to triacylglycerols, considerable amounts of cholesteryl esters and non-esterified fatty acids were detected in the lipids extracted from the inclinator muscle of fin. Finally, in situ hybridization localized the HSL1 transcripts to the adipocytes observed in the inclinator muscle of fin. Together, these results suggest that the inclinator muscle of fin is a lipid storage site that releases non-esterified fatty acids for aerobic fin movement, possibly through HSL-mediated lipolysis.

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Acknowledgments

The author would like to thank Dr. Hina Satone for kind instruction in dissection of Japanese flounder. We thank all the laboratory members for the technical advice and helpful discussion. This research was supported by the Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Young Scientists (B). Nos. 20780153 and 22780190, and Grant-in-Aid for Scientific Research (A). No. 21248027. This work was also partly supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences. A. K. was supported by a staff development scholarship from Naresuan University, Phitsanulok, Thailand.

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Correspondence to Gen Kaneko.

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Khieokhajonkhet, A., Kaneko, G., Ohara, K. et al. Hormone-sensitive lipase in Japanese flounder Paralichthys olivaceus: the potential function of the inclinator muscle of fin as a lipid storage site. Fish Sci 80, 341–351 (2014). https://doi.org/10.1007/s12562-013-0695-5

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Keywords

  • Hormone-sensitive lipase
  • Inclinator muscle of fin
  • Japanese flounder
  • Non-esterified fatty acid
  • Triacylglycerol