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Effects of feed restriction on the expression profiles of the glucose and fatty acid metabolism-related genes in rainbow trout Oncorhynchus mykiss muscle

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Abstract

Gene expression in the muscle of rainbow trout Oncorhynchus mykiss fed either ad libitum once a week (RF, restricted feed group) or ad libitum twice per day (control) was compared to identify those genes differently regulated during restricted feeding. After 1 month, the increase in the size of the RF fish was significantly less than that of the control fish. A microarray analysis of 37,394 unique sequences revealed that 2,701 transcripts differed by more than twofold between the control and RF groups. In the RF group, one and three genes involved in fatty acid β-oxidation were down- and up-regulated, respectively, and five glycolysis-related genes were down-regulated. The genes for growth hormone receptors-1 and -2 were up-regulated in the RF group. A number of adipocyte-related and adiponectin genes were down-regulated in the RF group, suggesting that lipid accumulation might be suppressed in this group. These results suggest that adiponectin and growth hormone signaling plays an active role in the control of energy metabolism during restricted food intake.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (A) [KAKENHI KIBAN KENKYU (A) 21248027] from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank the Yoshida Station of Tokyo University of Marine Science and Technology for rearing the fish used in this study. We also thank Dr. Mohamed Salem, West Virginia University, for the help with the microarray analysis.

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Authors and Affiliations

  1. Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan

    Hidehiro Kondo, Satomitsu Suda, Yuriko Kawana & Ikuo Hirono

  2. Laboratory of Food Function, Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan

    Reiko Nagasaka

  3. Laboratory of Marine Biochemistry, Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113-8657, Japan

    Gen Kaneko, Hideki Ushio & Shugo Watabe

Authors
  1. Hidehiro Kondo
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  2. Satomitsu Suda
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  3. Yuriko Kawana
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  4. Ikuo Hirono
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  5. Reiko Nagasaka
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  6. Gen Kaneko
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  7. Hideki Ushio
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  8. Shugo Watabe
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Correspondence to Hidehiro Kondo.

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Kondo, H., Suda, S., Kawana, Y. et al. Effects of feed restriction on the expression profiles of the glucose and fatty acid metabolism-related genes in rainbow trout Oncorhynchus mykiss muscle. Fish Sci 78, 1205–1211 (2012). https://doi.org/10.1007/s12562-012-0543-z

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  • DOI: https://doi.org/10.1007/s12562-012-0543-z

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