Selenoneine, total selenium, and total mercury content in the muscle of fishes

Abstract

Levels of the selenium-containing imidazole compound selenoneine and overall organic selenium were measured in the muscle of fishes by speciation analysis. The method involves monitoring 82Se levels by liquid chromatography inductively coupled plasma mass spectroscopy using a gel filtration column. Selenoneine levels were found to be highest in swordfish muscle (concentration 2.8 nmol/g tissue). The selenoneine contents of bigeye tuna, Pacific bluefin tuna, albacore, yellowfin tuna, and alfonsino muscle were 1.3–2.6 nmol/g tissue. In muscle of these fishes, most organic selenium (9–42%) was present as selenoneine. In other fish species, such as Pacific sardine, greeneye, skipjack, Pacific mackerel, horse mackerel, red sea bream, and Japanese barracuda, selenoneine levels were 0.1–1.4 nmol/g tissue, accounting for 3–34% of organic selenium. In contrast, muscle of Japanese conger, Japanese anchovy, chum salmon, Pacific saury, white croaker, and marbled sole contained levels of selenoneine below the level of detection (<0.05 nmol/g tissue). Mercury and selenium contents were 0.01–5.12 nmol/g tissue and 1.4–19.1 nmol/g tissue. The Se-to-Hg molar ratio varied from species to species, ranging from 1 for swordfish to 217 for marbled sole.

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Acknowledgments

This work was supported in part by grants from the Japan Society for the Promotion of Science, the Fisheries Research Agency, and the Ministry of Agriculture, Forestry, and Fisheries of Japan.

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Correspondence to Yumiko Yamashita.

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Yamashita, Y., Amlund, H., Suzuki, T. et al. Selenoneine, total selenium, and total mercury content in the muscle of fishes. Fish Sci 77, 679–686 (2011). https://doi.org/10.1007/s12562-011-0360-9

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Keywords

  • Selenoneine
  • Selenium
  • Mercury
  • Food safety
  • Muscle
  • Fish
  • Seafood