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Molecular cloning and tissue transcriptional analysis of a novel gene encoding proteasome activator PA28-α from common carp (Cyprinus carpio L.)

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  • Chemistry and Biochemistry
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Abstract

To identify the homolog of the PA28-α subunit, a leukocyte cDNA library of common carp was screened and the full-length cDNA sequence of the PSME1 gene coding for the PA28-α was obtained. It encompasses 1097 nucleotides (nt), with an open reading frame encoding 249 amino acids. The deduced protein sequence exhibits identities of 92, 78, 78, 77, 75, 56, 54, 53 and 54% with zebrafish, Atlantic salmon, northern pike, rainbow trout, rainbow smelt, mouse, pig, cattle and human PA28-α subunits, respectively. Multiple sequence alignment of the PA28-α protein of common carp and other known PA28 subunits indicates that it contains a PA28-α subunit-specific insert corresponding to the KEKE motif of the known PA28-α (Region B), a characteristic proline-rich motif (Region A), a potential protein kinase C recognition site (Region D), a conserved activation loop (Region C), and a highly homologous C-terminal region (Region E). Phylogenetic analysis reveals that the PA28-γ subunit is related to the presumed ancestor of the PA28-α and PA28-β subunits. In addition, the genomic DNA obtained by PCR is composed of eleven exons and ten introns with 3583 nt, typical of the PA28-α gene organization. Compared with the other known PA28-α genomic DNA, in spite of structural conservation through evolution, the fish PA28-α genes showed a much greater sequence divergence than their counterparts among mammals. Tissue transcriptional analysis of carp PA28-α indicated that it was visibly enhanced in tissues associated with immunity (gill, spleen, kidney and liver), and weakly transcribed in muscle, brain and heart.

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Acknowledgments

We are grateful to Dr Su Zhong of the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, China, for reviewing the manuscript. This work was supported by grants from the National Natural Science Foundation of China (30200210, 30571433).

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Authors and Affiliations

  1. Ministry of Education Key Laboratory for Zoonosis, Institute of Zoonosis, Jilin University, Changchun, 130062, People’s Republic of China

    Yeping Tan, Qiang Lu, Lianrui Li & Yanlei Zhu

  2. College of Veterinary Medicine, South China Agricultural University, Tianhe District, Guangzhou, 510642, People’s Republic of China

    Yeping Tan

  3. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Yanchangbao, Lanzhou, 730046, People’s Republic of China

    Baoquan Fu

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  1. Yeping Tan
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  2. Qiang Lu
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Correspondence to Qiang Lu.

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Tan, Y., Lu, Q., Li, L. et al. Molecular cloning and tissue transcriptional analysis of a novel gene encoding proteasome activator PA28-α from common carp (Cyprinus carpio L.). Fish Sci 76, 511–519 (2010). https://doi.org/10.1007/s12562-010-0226-6

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  • DOI: https://doi.org/10.1007/s12562-010-0226-6

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