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Artificially induced tetraploid masu salmon have the ability to form primordial germ cells

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  • Aquaculture
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Abstract

Diploid gametes generated with tetraploid animals are a stepping stone to improving chromosome manipulation techniques. However, artificially induced tetraploid individuals generally die soon after hatching. Diploid gametes could be induced by in vivo cultures of tetraploid primordial germ cells (PGCs) through germ-line chimera. In the present study, characteristics of PGCs were studied in inviable tetraploid masu salmon, Oncorhynchus masou. Histological observation of tetraploid embryos revealed that the same or smaller numbers of PGCs were observed and they migrate into the genital ridges as did diploid PGCs during gonadogenesis. By whole-mount in situ hybridization using vasa messenger RNA (mRNA), 4–35 vasa-positive signals were detected in a pair of genital ridges of tetraploids. By cytological observation of genital ridge cell suspensions, several large round cells were observed, some of which extended pseudopodia. They also contained large nuclei and round granules in their cytoplasm, characteristics of PGCs. As the results suggest that inviable artificial tetraploids have PGCs, we expect to achieve diploid gamete production through surrogate propagation and tetraploid fish production.

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Acknowledgments

We thank the Hokkaido Fish Hatchery Mori Research Branch for providing masu salmon. We also thank Mr. S. Kimura, Ms. C. Nishida, Ms. M. Takagi, and members of the Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere and the Laboratory of Breeding Sciences, Graduate School of Fisheries Sciences, Hokkaido University for useful discussions. This study was supported in part by grants-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (nos. 13460079 and 14360105).

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Authors and Affiliations

  1. Laboratory of Breeding Science, Graduate School of Fisheries Science, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Suzu Sakao, Takafumi Fujimoto & Katsutoshi Arai

  2. Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan

    Terumasa Kobayashi & Goro Yoshizaki

  3. Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Kameda, Hokkaido, 041-1105, Japan

    Etsuro Yamaha

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  1. Suzu Sakao
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  2. Takafumi Fujimoto
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  3. Terumasa Kobayashi
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  4. Goro Yoshizaki
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  5. Etsuro Yamaha
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  6. Katsutoshi Arai
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Correspondence to Suzu Sakao.

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Sakao, S., Fujimoto, T., Kobayashi, T. et al. Artificially induced tetraploid masu salmon have the ability to form primordial germ cells. Fish Sci 75, 993–1000 (2009). https://doi.org/10.1007/s12562-009-0124-y

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  • DOI: https://doi.org/10.1007/s12562-009-0124-y

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