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Molecular cloning and expression analysis of the BPI/LBP cDNA and its gene from ayu Plecoglossus altivelis altivelis

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Abstract

Bactericidal/permeability-increasing protein and lipopolysaccharide-binding protein (BPI/LBP) are important components of the mammalian innate defense system against Gram-negative bacterial infections. Ayu Plecoglossus altivelis altivelis BPI/LBP cDNA and its gene were cloned. The open reading frame of ayu BPI/LBP encoded a protein of 471 amino acids that contains a signal peptide and the BPI/LBP/ cholesteryl ester transfer protein-specific sequence motifs, BPI1 and BPI2. The basic amino acid residues in the LPS-binding domains of human Homo sapiens BPI and LBP and bony fishes BPI/LBP were well conserved in ayu BPI/LBP. The ayu BPI/LBP gene is composed of 15 exons and 14 introns. Ayu BPI/LBP mRNA was predominantly expressed in gill, intestine, kidney, spleen, and skin. At 4 and 7 days after the immersion challenge of Flavobacterium psychrophilum, the ayu BPI/LBP mRNA expression in the whole blood increased about fivefold in comparison with control (no challenge). Because ayu BPI/LBP mRNA increased before ayu began to die following exposure to F. psychrophilum, ayu BPI/LBP gene transcripts might be useful as a marker of the response to infectious Gram-negative bacteria or as a health status indicator.

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Authors and Affiliations

  1. Gunma Prefectural Fisheries Experiment Station, Maebashi, Gunma, 371-0036, Japan

    Kyuma Suzuki & Hideki Tanaka

  2. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo, 108-8477, Japan

    Kyuma Suzuki & Takayuki Katagiri

  3. Gunma Prefectural Fisheries Experiment Station Kawaba Branch, Kawaba, Gunma, 378-0114, Japan

    Shotaro Izumi

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  1. Kyuma Suzuki
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  2. Shotaro Izumi
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Correspondence to Kyuma Suzuki.

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Suzuki, K., Izumi, S., Tanaka, H. et al. Molecular cloning and expression analysis of the BPI/LBP cDNA and its gene from ayu Plecoglossus altivelis altivelis . Fish Sci 75, 673–681 (2009). https://doi.org/10.1007/s12562-009-0072-6

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  • DOI: https://doi.org/10.1007/s12562-009-0072-6

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