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Expressed sequence tag analysis of phyllosomas and hemocytes of Japanese spiny lobster Panulirus japonicus

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Abstract

Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein, antimicrobial peptide, and a few putative defense-related proteins.

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Acknowledgments

This study was supported in part by grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan and the Grants in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan

    Duangjai Pisuttharachai, Motoshige Yasuike, Hidehiro Kondo, Takashi Aoki & Ikuo Hirono

  2. Fisheries Research Agency, 6-31-1, Nagai, Yokosuka, Kanagawa, 238-0316, Japan

    Hideaki Aono

  3. Minami-izu, Sea-Farming Center, Fisheries Research Agency, 183-2 Irozaki, Minami-izu, Shizuoka, 415-0156, Japan

    Keisuke Murakami

Authors
  1. Duangjai Pisuttharachai
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  2. Motoshige Yasuike
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  3. Hideaki Aono
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  4. Keisuke Murakami
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  5. Hidehiro Kondo
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  6. Takashi Aoki
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  7. Ikuo Hirono
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Correspondence to Ikuo Hirono.

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Pisuttharachai, D., Yasuike, M., Aono, H. et al. Expressed sequence tag analysis of phyllosomas and hemocytes of Japanese spiny lobster Panulirus japonicus . Fish Sci 75, 195–206 (2009). https://doi.org/10.1007/s12562-008-0027-3

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  • DOI: https://doi.org/10.1007/s12562-008-0027-3

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