Abstract
Cost-effective yet efficient designs are critical to the success of animal studies. We propose a two-stage design for cost-effectiveness animal studies with continuous outcomes. Given the data from the two-stage design, we derive the exact distribution of the test statistic under null hypothesis to appropriately adjust for the design’s adaptiveness. We further generalize the design and inferential procedure to the K-sample case with multiple comparison adjustment. We conduct simulation studies to evaluate the small sample behavior of the proposed design and test procedure. The results indicate that the proposed test procedure controls the type I error rate for the one-sample design and the family-wise error rate for K-sample design very well, whereas the naive approach that ignores the design’s adaptiveness due to the interim look severely inflates the type I error rate or family-wise error rate. Compared with the standard one-stage design, the proposed design generally requires a smaller sample size.
Similar content being viewed by others
References
Aban IB, George B (2015) Statistical considerations for preclinical studies. Exp Neurol 270:82–87
Abe K, Yamashita T, Takizawa S, Kuroda S, Kinouchi H, Kawahara N (2012) Stem cell therapy for cerebral ischemia: from basic science to clinical applications. J Cereb Blood Flow Metab 32(7):1317–1331
Berry SM, Carlin BP, Lee JJ, Muller P (2010) Bayesian adaptive methods for clinical trials. Chapman & Hall, London
Blakesley RE, Mazumdar S, Dew MA, Houck PR, Tang G, Reynolds CF III, Butters MA (2009) Comparisons of methods for multiple hypothesis testing in neuropsychological research. Neuropsychology 23(2):255
Cai C, Ning J, Huang X (2016) A Bayesian multi-stage cost-effectiveness design for animal studies in stroke research. Stat Methods Med Res. https://doi.org/10.1177/0962280216657853
Chen TT (1997) Optimal three-stage designs for phase II cancer clinical trials. Stat Med 16(23):2701–2711
Chow SC, Chang M (2011) Adaptive design methods in clinical trials. Chapman & Hall, London
Chow SC, Chang M et al (2008) Adaptive design methods in clinical trials: a review. Orphanet J Rare Dis 3(11):169–190
de Aguilar-Nascimento JE (2005) Fundamental steps in experimental design for animal studies. Acta Cir Bras 20(1):2–3
Dunn OJ (1961) Multiple comparisons among means. J Am Stat Assoc 56(293):52–64
Food and Drug Administration (2010) Guidance for industry: adaptive design clinical trials for drugs and biologics. Food and Drug Administration, Washington DC
Gallo P, Chuang-Stein C, Dragalin V, Gaydos B, Krams M, Pinheiro J (2006) Adaptive designs in clinical drug development—an executive summary of the PhRMA working group. J Biopharm Stat 16(3):275–283
Hackam DG (2007) Translating animal research into clinical benefit. BMJ 7586:163
Henderson VC, Kimmelman J, Fergusson D, Grimshaw JM, Hackam DG (2013) Threats to validity in the design and conduct of preclinical efficacy studies: a systematic review of guidelines for in vivo animal experiments. PLoS Med 10(7):e1001489
Hess KR (2011) Statistical design considerations in animal studies published recently in cancer research. Cancer Res 71(2):625
Hochberg Y (1988) A sharper Bonferroni procedure for multiple tests of significance. Biometrika 75(4):800–802
Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70
Hommel G (1988) A stagewise rejective multiple test procedure based on a modified Bonferroni test. Biometrika 75(2):383–386
Jennison C, Turnbull BW (1999) Group sequential methods with applications to clinical trials. CRC Press, Boca Raton
Kilkenny C, Parsons N, Kadyszewski E, Festing MF, Cuthill IC, Fry D, Hutton J, Altman DG (2009) Survey of the quality of experimental design, statistical analysis and reporting of research using animals. PLoS ONE 4(11):e7824
Lachin JM (2005) A review of methods for futility stopping based on conditional power. Stat Med 24(18):2747–2764
Landis SC, Amara SG, Asadullah K, Austin CP, Blumenstein R, Bradley EW, Crystal RG, Darnell RB, Ferrante RJ, Fillit H et al (2012) A call for transparent reporting to optimize the predictive value of preclinical research. Nature 490(7419):187–191
Lewis RJ, Berry DA (1994) Group sequential clinical trials: a classical evaluation of Bayesian decision-theoretic designs. J Am Stat Assoc 89(428):1528–1534
Lin PE (1972) Some characterizations of the multivariate t distribution. J Multivar Anal 2(3):339–344
Lin SP, Chen TT (2000) Optimal two-stage designs for phase II clinical trials with differentiation of complete and partial responses. Commun Stat 29(5–6):923–940
Lu Y, Jin H, Lamborn KR (2005) A design of phase II cancer trials using total and complete response endpoints. Stat Med 24(20):3155–3170
Macleod MR (2014) Preclinical research: design animal studies better. Nature 510(7503):35
Majid A, Bae ON, Redgrave J, Teare D, Ali A, Zemke D (2015) The potential of adaptive design in animal studies. Int J Mol Sci 16(10):24048–24058
Perrin S (2014) Preclinical research: make mouse studies work. Nature 507(7493):423–425
Pocock SJ (1977) Group sequential methods in the design and analysis of clinical trials. Biometrika 64(2):191–199
Simon R (1989) Optimal two-stage designs for phase II clinical trials. Control Clin Trials 10(1):1–10
Whitehead J (1997) The design and analysis of sequential clinical trials. Wiley, Hoboken
Acknowledgements
The authors thank the editor, the associate editor, and two reviewers for their constructive comments that have greatly improved the initial version of this paper. The work was supported in part by the U.S. National Institutes of Health Grants UL1 TR000371, CA193878, and CA016672.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Cai, C., Piao, J., Ning, J. et al. Efficient Two-Stage Designs and Proper Inference for Animal Studies. Stat Biosci 10, 217–232 (2018). https://doi.org/10.1007/s12561-017-9212-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12561-017-9212-1