Abstract
This study investigated the obstacles in detecting enteric viruses from berry fruits, which are on the one hand often associated with outbreaks of viral enteric disease, and on the other hand recognized as a challenging food matrix for molecular detection of enteric viruses. According to the ISO 15216 protocol, for soft fruit samples, virus extraction is by elution with agitation followed by precipitation with polyethylene glycol/NaCl. As a result, first, the phenolic content in the berry eluate was found to be weakly correlated with the detection of coliphage MS2 spiked in the berry samples. Second and more importantly, it was observed that the gel-like pellets formed after precipitation could entrap considerable portions of viruses from being further purified and recovered for detection, suggesting that the low virus detection sensitivity from berries is largely due to the pectin content with complicated chemical structures in the berry fruits. Future research is needed to solve this problem in a targeted way.
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Funding
This study was supported by a Ministry of Education (MOE) academic research fund (AcRF) TIER 1 project “Study of important foodborne viruses from relevant foods in Singapore” (Jan 2019 to Dec 2021).
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Conceptualization: MYZ and DL; Methodology: MYZ and DL; Software: MYZ; Validation: MYZ and DL; Resources: MYZ and DL; Writing—Original draft preparation: MYZ; Writing—Review and Editing: MYZ and DL; Visualization: MYZ; Supervision: DL; Funding acquisition: DL.
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Zhao, M.Y., Li, D. Discovery of Components Acting as the Obstacles in the Detection of Enteric Viruses from Berries. Food Environ Virol 12, 191–197 (2020). https://doi.org/10.1007/s12560-020-09428-y
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DOI: https://doi.org/10.1007/s12560-020-09428-y