Abstract
The shrimp farming has been converted into a mature aquaculture industry dealing with over millions of metric tonnes of processed commodities. Nevertheless, the global shrimp productions are constantly threatened by disease outbreaks, mainly triggered by rapidly disseminating viruses. Infectious myonecrosis virus (IMNV) is one of these epizootic agents affecting shrimp production in Brazil, of which no treatment exists. Herein, the antiviral activity against IMNV of an eicosapeptide, named Ctn[15-34], derived from a member of the cathelicidin family of antimicrobial peptides, was demonstrated. Cultures of hemocytes from Litopenaeus vannamei were established that support IMNV replication and infectivity titration. The cytotoxic effect of IMNV in culture and the in vitro anti-IMNV activity of Ctn[15-34] were assessed using a high-sensitive fluorescent-based method in combination with quantitative PCR. The Ctn[15-34] (<12.5 µM) neutralized the toxic effects of IMNV at loads sufficient to kill 50% of shrimp hemocytes. This study reported for the first time the replication of IMNV in vitro and the employment of a straightforward methodology to assess cell viability and viral/antiviral activities. In addition, it provided the basis for the development of the anti-infective multi-effector Ctn[15-34] eicosapeptide and analogs as components of antiviral formulations against shrimp viral diseases.
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Acknowledgements
This research study was supported by the funding from the Program on Toxinology (Issue 2010), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Ministry of Education (ME) of the Federal Government of Brazil, granted to G. Rádis-Baptista. P.R.N. Vieira-Girão is a recipient of fellowships from the CAPES.C.B. Falcão is associate researcher from the National Program for Post-Doctorates (CAPES/ME) at the Postgraduate Program in Pharmaceutical Sciences, Faculty of Pharmacy, Dentistry, and Nursing, the Federal University of Ceara.
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Vieira-Girão, P.R.N., Falcão, C.B., Rocha, I.R.C.B. et al. Antiviral Activity of Ctn[15-34], A Cathelicidin-Derived Eicosapeptide, Against Infectious Myonecrosis Virus in Litopenaeus vannamei Primary Hemocyte Cultures. Food Environ Virol 9, 277–286 (2017). https://doi.org/10.1007/s12560-017-9285-5
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DOI: https://doi.org/10.1007/s12560-017-9285-5