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Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley, Nepal

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Abstract

Aichi virus 1 genomes were detected by quantitative PCR in groundwater from shallow dug (10/22) and tube wells (1/15), river water (14/14), and sewage (1/1), with the maximum concentration of 4.0 × 109 copies/l. Nucleotide sequencing analysis demonstrated the prevalence of genotype B in the virus positive samples.

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Acknowledgements

This study was partially supported by the Grant-in-Aid for the Global COE Program “Evolution of Research and Education on Integrated River Basin Management in Asian Region” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Authors and Affiliations

  1. Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, Yamanashi, 400-8511, Japan

    Eiji Haramoto

  2. Division of Environmental Engineering, Faculty of Engineering, Hokkaido University, North 13 West 8, Kita-ku, Sapporo, Hokkaido, 060-8628, Japan

    Masaaki Kitajima

Authors
  1. Eiji Haramoto
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  2. Masaaki Kitajima
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Corresponding author

Correspondence to Eiji Haramoto.

Additional information

Nucleotide Sequence Accession Numbers

The nucleotide sequences determined in this study have been deposited in the GenBank database under accession numbers LC200515–LC200529.

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Haramoto, E., Kitajima, M. Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley, Nepal. Food Environ Virol 9, 350–353 (2017). https://doi.org/10.1007/s12560-017-9283-7

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  • DOI: https://doi.org/10.1007/s12560-017-9283-7

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