Abstract
Essential oils (EOs) and some of their main compounds have demonstrated extensive antimicrobial activity in a wide range of food spoilage or pathogenic fungi, yeast and bacteria. The aim of this study was to assess the antiviral activity of Zataria multiflora Boiss. (zataria) and Origanum vulgare (oregano) EOs on hepatitis A virus (HAV) and the effect of thymol, an active compound of Thymus vulgaris and oregano, on norovirus surrogates, feline calicivirus (FCV) and murine norovirus (MNV), and HAV. Initially, each virus at titers of ca. 6 log TCID50/ml was exposed to different concentrations of natural compounds and incubated for 2 h at 37 °C. Treatment with oregano and zataria EOs resulted in slight reductions on HAV infectivity with a maximum reduction of less than 0.5 log TCID50/ml at 0.1 % zataria EO. Thymol was effective in reducing the titers of norovirus surrogates in a dose-dependent manner. Concentrations of thymol at 0.5 and 1 % reduced FCV titers to undetectable levels, while for MNV, thymol at concentrations of 1 and 2 % resulted in reductions of 1.66 and 2.45 log TCID50/ml, respectively. However, for HAV, no effect was observed at any of the concentrations tested. These results improve the knowledge about the antiviral activity of EO and their compounds and their potential in food sanitation.
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References
Anonymous. (2013). Surveillance for foodborne disease outbreaks—United States, 2009–2010. Morbidity and Mortality Weekly Report, 62, 41–47.
Burt, S. (2004). Essential oils: Their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94, 223–253.
Collier, M. G., Khudyakov, Y. E., Selvage, D., Adams-Cameron, M., Epson, E., Cronquist, A., et al. (2014). Outbreak of hepatitis A in the USA associated with frozen pomegranate arils imported from Turkey: An epidemiological case study. The Lancet Infectious Diseases, 14, 976–981.
EFSA (2014). The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA Journal, 12(2), 3547, 312.
El Moussaoui, N., Sanchez, G., El Ouardy, K., Idaomar, M., Mansour, A. I., Abrini, J., & Aznar, R. (2013). Antibacterial and antiviral activities of essential oils of northern moroccan plants. British Biotechnology Journal, 3(3), 318–331.
Elizaquível, P., Azizkhani, M., Aznar, R., & Sanchez, G. (2013). The effect of essential oils on norovirus surrogates. Food Control, 32, 275–278.
Garozzo, A., Timpanaro, R., Bisignano, B., Furneri, P. M., Bisignano, G., & Castro, A. (2009). In vitro antiviral activity of Melaleuca alternifolia essential oil. Letters in Applied Microbiology, 49, 806–808.
Gilling, D. H., Kitajima, M., Torrey, J. R., & Bright, K. R. (2014a). Antiviral efficacy and mechanisms of action of oregano essential oil and its primary component carvacrol against murine norovirus. Journal of Applied Microbiology, 116, 1149–1163.
Gilling, D. H., Kitajima, M., Torrey, J. R., & Bright, K. R. (2014b). Mechanisms of antiviral action of plant antimicrobials against murine norovirus. Applied and Environmental Microbiology, 80, 4898–4910.
Guzman-Herrado, B., Jensvoll, L., Einöder-Moreno, M., Lange, H., Myking, S., Nygård, K., Stene-Johansen, K., & Vold, L. (2014). Ongoing hepatitis A outbreak in Europe 2013 to 2014: Imported berry mix cake suspected to be the source of infection in Norway. Eurosurveillance, 19(15): pii = 20775. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=20775.
Gyawali, R., & Ibrahim, S. A. (2014). Natural products as antimicrobial agents. Food Control, 46, 412–429.
Kovac, K., Diez-Valcarce, M., Raspor, P., Hernández, M., & Rodríguez-Lázaro, D. (2012). Natural plant essential oils do not inactivate non-enveloped enteric viruses. Food and Environmental Virology, 4, 209–212.
Lu, Y., & Wu, C. (2010). Reduction of Salmonella enterica contamination on grape tomatoes by washing with thyme oil, thymol, and carvacrol as compared with chlorine treatment. Journal of Food Protection, 73(12), 2270–2275.
Pilau, M. R., Alves, S. H., Weiblen, R., Arenhart, S., Cueto, A. P., & Lovato, L. T. (2011). Antiviral activity of the Lippia graveolens (Mexican oregano) essential oil and its main compound carvacrol against human and animal viruses. Brazilian Journal of Microbiology, 42(4), 1616–1624.
Pintó, R. M., Diez, J. M., & Bosch, A. (1994). Use of the colonic carcinoma cell line CaCo-2 for in vivo amplification and detection of enteric viruses. Journal of Medical Virology, 44, 310–315.
Ramos, M., Jiménez, A., Peltzer, M., & Garrigós, M. C. (2012). Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. Journal of Food Engineering, 109, 513–519.
Sánchez, G., Aznar, R., Martínez, A., & Rodrigo, D. (2011). Inactivation of human and murine norovirus by high-pressure processing. Foodborne Pathogens and Disease, 8, 249–253.
Sánchez, C., Aznar, R., & Sánchez, G. (2015). The effect of carvacrol on enteric viruses. International Journal of Food Microbiology, 192, 72–76.
Sprenger, M. (2014). More can be done to stop ‘silent disease’ of hepatitis. The Parliament Magazine394/395: 9. http://viewer.zmags.com/publication/39b84223#/39b84223/9 (accessed Oct 27, 2014).
Su, X., & D’Souza, D. H. (2011). Grape seed extract for control of human enteric viruses. Applied and Environmental Microbiology, 77, 3982–3987.
Wenzel, J. J., Schemmerer, M., Oberkofler, H., Koidl, C., & Allerberger, F. (2014). Hepatitis A Outbreak in Europe: Imported Frozen Berry Mix Suspected to be the Source of At least One Infection in Austria in 2013. Food Environmental Virology, 6(4), 297–300.
Acknowledgments
G. Sánchez was supported by the “Ramón y Cajal” Young Investigator program of the Spanish Ministry of Economy and Competitiveness. This study was supported by grant AGL2009-08603 from the Spanish Ministry of Science and Innovation, and ACOMP/2010/279 and ACOMP/2012/199 from the Generalitat Valenciana. We thank Carlos Sánchez for technical support.
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Sánchez, G., Aznar, R. Evaluation of Natural Compounds of Plant Origin for Inactivation of Enteric Viruses. Food Environ Virol 7, 183–187 (2015). https://doi.org/10.1007/s12560-015-9181-9
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DOI: https://doi.org/10.1007/s12560-015-9181-9