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Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel

Food and Environmental Virology volume 6, pages 290–296 (2014)Cite this article

Abstract

Outbreaks of viral diseases are frequently associated with the consumption of minimally processed shellfish. Among the viruses in these outbreaks, hepatitis A virus (HAV) and human norovirus (NoV) have been increasingly reported as the most common food-borne pathogens. These viruses must be concentrated in tested samples in order to be detected. In this study, a method for the detection of NoV and HAV in shellfish using an immuno-magnetic separation (IMS) procedure combined with reverse transcriptase (RT)-PCR was developed. The IMS/RT-PCR method was applied to investigate the recovery rates of HAV, NoV GI.1, and GII.4 from oyster and mussel. Based on IMS/RT-PCR results, recovery rates for HAV from oyster and mussel test samples were 2.4 and 1.1 %, respectively. The NoV GI.1 recovery rates from oyster and mussel samples were 4.9–9.2 % (mean 6.9 %) and 4.3–8.6 % (mean 6.2 %), respectively, and the NoV GII.4 recovery rates were 8.8 and 8.5 %, respectively. These results verified that HAV, NoV GI.1, and GII.4 can be detected in all the test samples using the IMS/RT-PCR method, although the three inoculated viruses were recovered with low efficiency. In conclusion, the IMS/RT-PCR method can be used to efficiently and rapidly detect viruses such as HAV and NoV in shellfish such as oyster and mussel.

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Acknowledgments

This research was supported by the Fishery Commercialization Technology Development Program (#112089-3) of the Ministry for Food, Agriculture, Forestry, and Fisheries, Republic of Korea.

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  1. School of Food Science and Technology, Chung-Ang University, 4726 Seodongdaero, Ansung, Gyunggi-do, 456-756, Republic of Korea

    Ji-Hyoung Ha, Changsun Choi & Sang-Do Ha

  2. Department of Food Science and Technology, Chung-Ang University, 72-1 Nae-ri, Daeduk-myun, Ansung, Gyunggi-do, 456-756, Republic of Korea

    Sang-Do Ha

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  1. Ji-Hyoung Ha
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  2. Changsun Choi
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Correspondence to Sang-Do Ha.

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Ha, JH., Choi, C. & Ha, SD. Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel. Food Environ Virol 6, 290–296 (2014). https://doi.org/10.1007/s12560-014-9156-2

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  • DOI: https://doi.org/10.1007/s12560-014-9156-2

Keywords

  • Hepatitis A virus
  • Immunomagnetic separation
  • Mussel
  • Norovirus
  • Oyster