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Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors

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Abstract

In the last decades, a promising breakthrough in fluorescence imaging was represented by the advent of super-resolution microscopy (SRM). Super-resolution techniques recently became a popular method to study sub-cellular structures, providing a successful approach to observe cytoskeletal and focal adhesion proteins. Among the SR techniques, single-molecule localization microscopy plays a significant role due to its ability to unveil structures and molecular organizations in biological systems. Furthermore, since they provide information at the molecular level, these techniques are increasingly being used to study the stoichiometry and interaction between several membrane channel proteins and their accessory subunits. The aim of this review is to describe the single-molecule localization-based techniques and their applications relevant to cytoskeletal structures and membrane complexes in order to provide as future prospective an overall picture of their correlation with the mechanosensor channel expression and activity.

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Acknowledgments

The authors acknowledge the Nanoscopy group and the Nikon imaging center at the Istituto Italiano di Tecnologia.

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Authors and Affiliations

  1. Biophysics Institute (IBF), National Research Council (CNR), Genoa, Italy

    R. Magrassi, S. Scalisi & F. Cella Zanacchi

  2. Nanoscopy and NIC@IIT, Istituto Italiano di tecnologia, Genoa, Italy

    R. Magrassi & F. Cella Zanacchi

  3. DIFI, University of Genoa, Genoa, Italy

    S. Scalisi

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  3. F. Cella Zanacchi
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Correspondence to R. Magrassi or F. Cella Zanacchi.

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Magrassi, R., Scalisi, S. & Cella Zanacchi, F. Single-molecule localization to study cytoskeletal structures, membrane complexes, and mechanosensors. Biophys Rev 11, 745–756 (2019). https://doi.org/10.1007/s12551-019-00595-2

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