Structural atlas of dynein motors at atomic resolution
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Dynein motors are biologically important bio-nanomachines, and many atomic resolution structures of cytoplasmic dynein components from different organisms have been analyzed by X-ray crystallography, cryo-EM, and NMR spectroscopy. This review provides a historical perspective of structural studies of cytoplasmic and axonemal dynein including accessory proteins. We describe representative structural studies of every component of dynein and summarize them as a structural atlas that classifies the cytoplasmic and axonemal dyneins. Based on our review of all dynein structures in the Protein Data Bank, we raise two important points for understanding the two types of dynein motor and discuss the potential prospects of future structural studies.
KeywordsDynein Dynein subunits Axonemal dynein light chain-1 Molecular motor Structural analysis
We thank the staff of the beamline BL44XU at SPring-8, Japan, for the help during data collection and Prof. Toshiki Yagi for providing the plasmid used in this study.
This work was supported by a Grant-in-Aid for Scientific Research B (JP26291014) from the Japan Society for the Promotion of Science (JSPS) and JSPS Fellows Number JP17J07553.
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Conflict of interest
Akiyuki Toda declares that he has no conflict of interest. Hideaki Tanaka declares that he has no conflict of interest. Genji Kurisu declares that he has no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Fan J-S, Zhang Q, Tochio H, et al (2001) Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain11 Edited by P. E. Wright. J Mol Biol 306:97–108. doi: https://doi.org/10.1006/jmbi.2000.4374
- Inaba K (2007) Molecular basis of sperm flagellar axonemes: structural and evolutionary aspects. In: Annals of the New York Academy of Sciences. pp 506–526Google Scholar
- Rapali P, Radnai L, Süveges D et al (2011) Directed evolution reveals the binding motif preference of the LC8/DYNLL hub protein and predicts large numbers of novel binders in the human proteome. PLoS One. https://doi.org/10.1371/journal.pone.0018818
- Williams JC, Siglin AE, Lightcap CM, Dawn A (2012) Structural analysis of dynein intermediate and light chains. In: Dyneins. pp 156–189Google Scholar