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Nonlinear optical microscopy in decoding arterial diseases

Biophysical Reviews volume 4, pages 323–334 (2012)Cite this article

Abstract

Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visualize pathological changes in the extracellular matrix caused by atherosclerosis progression. These techniques in general require minimal tissue preparation and offer rapid three-dimensional imaging. The capability of label-free microscopic imaging enables disease impact to be studied directly on the bulk artery tissue, thus minimally perturbing the sample. In this review, we look at recent progress in applications related to arterial disease imaging using various forms of NLOM.

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Authors and Affiliations

  1. National Research Council Canada, Institute for Biodiagnostics, 435 Ellice Avenue, Winnipeg, Manitoba, Canada, R3B 1Y6

    Alex C.-T. Ko & Michael G. Sowa

  2. National Research Council Canada, Steacie Institute for Molecular Sciences, 100 Sussex Drive, Ottawa, Ontario, Canada, K1A 0R6

    Andrew Ridsdale & Albert Stolow

  3. Department of Electrical and Computer Engineering, University of Manitoba, 75A Chancellor’s Circle, Winnipeg, Manitoba, Canada, R3T 5V6

    Leila B. Mostaço-Guidolin & Arkady Major

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  1. Alex C.-T. Ko
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  2. Andrew Ridsdale
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  3. Leila B. Mostaço-Guidolin
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  4. Arkady Major
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  5. Albert Stolow
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  6. Michael G. Sowa
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Correspondence to Alex C.-T. Ko.

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Ko, A.CT., Ridsdale, A., Mostaço-Guidolin, L.B. et al. Nonlinear optical microscopy in decoding arterial diseases. Biophys Rev 4, 323–334 (2012). https://doi.org/10.1007/s12551-012-0077-8

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