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Biophysical Reviews

, Volume 4, Issue 4, pp 323–334 | Cite as

Nonlinear optical microscopy in decoding arterial diseases

  • Alex C.-T. Ko
  • Andrew Ridsdale
  • Leila B. Mostaço-Guidolin
  • Arkady Major
  • Albert Stolow
  • Michael G. Sowa
Review

Abstract

Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visualize pathological changes in the extracellular matrix caused by atherosclerosis progression. These techniques in general require minimal tissue preparation and offer rapid three-dimensional imaging. The capability of label-free microscopic imaging enables disease impact to be studied directly on the bulk artery tissue, thus minimally perturbing the sample. In this review, we look at recent progress in applications related to arterial disease imaging using various forms of NLOM.

Keywords

Atherosclerosis Nonlinear optical microscopy Second-harmonic generation Two-photon excited fluorescence Coherent anti-Stokes Raman Artery 

Notes

Conflicts of interest

None.

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Copyright information

© © Her Majesty the Queen in Right of Canada 2012 2012

Authors and Affiliations

  • Alex C.-T. Ko
    • 1
  • Andrew Ridsdale
    • 2
  • Leila B. Mostaço-Guidolin
    • 3
  • Arkady Major
    • 3
  • Albert Stolow
    • 2
  • Michael G. Sowa
    • 1
  1. 1.National Research Council CanadaInstitute for BiodiagnosticsWinnipegCanada
  2. 2.National Research Council CanadaSteacie Institute for Molecular SciencesOttawaCanada
  3. 3.Department of Electrical and Computer EngineeringUniversity of ManitobaWinnipegCanada

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