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Degradation of deoxynivalenol by a microbial consortia C1 from duck intestine

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Abstract

Deoxynivalenol (DON), one of the most widespread mycotoxins in food and feed, poses a persistent health threat to humans and farm animals, and is difficult to eliminate. The utilization of the biotransformation mechanism by microorganisms to detoxify DON is a promising strategy. Although individual strains are capable of DON degradation, their isolation and purification are challenging and time-consuming. Recently, the microbial consortia concept has been proposed, owing to their ability to perform more complex tasks and are more tolerant to environmental changes than individual strains or species. In this study, the novel microbial consortia C1 that could efficiently convert DON to de-epoxy DON (DOM-1) was screened from the cecum contents of ducks. After 24 h anaerobic incubation, 100 μg/ml DON was completely degraded by C1. In vitro, C1 can effectively degrade DON in corn steep liquor (CSL) with an efficiency of 49.44% within 14 days. Furthermore, C1 effectively alleviated the DON poisoning in mice. After C1 treatment, the serum DON level decreased by 40.39%, and the reduction in serum total protein and albumin levels were mitigated. Additionally, C1 is effective in protecting the mouse liver against 5 mg/kg DON. These findings suggest that C1 could be a promising DON biological detoxifier and provide novel microbial resources for preventing DON contamination.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We appreciate the help of Yu Wu from the Academy of National Food and Strategic Reserves Administration for valuable analysis with the mass spectrometry.

Funding

This work was supported by the National Key Research and Development Program of China: No.2016YFE0113300; Central Public-interest Scientific Institution Basal Research Fund (No. 1610382022007); European Union’s Horizon 2020 research and innovation program under grant agreement: No. 678012.

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Authors and Affiliations

  1. Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, China

    Yunduo Zheng, Boquan Gao, Jianwen Wu, Xiumin Wang, Bing Han, Hui Tao, Jie Liu, Zhenlong Wang & Jinquan Wang

  2. Laboratory of Pet Nutrition and Food, Institute of Feed Research, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing, 100081, China

    Yunduo Zheng, Boquan Gao, Jianwen Wu, Xiumin Wang, Bing Han, Hui Tao, Jie Liu, Zhenlong Wang & Jinquan Wang

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  1. Yunduo Zheng
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  2. Boquan Gao
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  4. Xiumin Wang
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  8. Zhenlong Wang
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Contributions

J. Q. W. and Z. L. W. conceived and designed the experiments; Y. D. Z., Z. L. W., B. Q. G., and J. W. W. conducted the experiments; H. T., B. H., X. M. W., and J. L. supervised the work, provided some data analysis, and revised the final version of the manuscript; Z. L. W., and J. Q. W. wrote the manuscript.

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Correspondence to Zhenlong Wang or Jinquan Wang.

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Zheng, Y., Gao, B., Wu, J. et al. Degradation of deoxynivalenol by a microbial consortia C1 from duck intestine. Mycotoxin Res 40, 147–158 (2024). https://doi.org/10.1007/s12550-023-00511-4

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