Preliminary sampling of aflatoxin M1 contamination in raw milk from dairy farms using feed ingredients from Rwanda

Abstract

Milk is susceptible to aflatoxin M1 (AFM₁) contamination when dairy cattle consume feed contaminated with aflatoxins and is considered as a public health concern. This pilot study assessed the prevalence and amount of total aflatoxin contamination in commercially available dairy feed and the corresponding AFM₁ contamination in raw milk from samples collected at farms using local, commercially available dairy feed across Rwanda’s five provinces. The inclusion criteria to select dairy farm participants were (1) to have at least two cows and (2) use of commercially prepared dairy feeds. Importantly, the majority of cattle rearing households in Rwanda rely principally on grazing or other freely available feedstock, rather than on commercially prepared feeds. In total, 170 raw milk samples were collected during one sampling period from dairy farms using commercially prepared dairy feeds. In addition, 154 dairy feed samples were collected simultaneously with the milk samples. These farms were previously targeted in a larger study measuring aflatoxin contamination of Rwandan feeds and feed ingredients. The mean AFM₁ concentration in these samples was 0.89 ± 1.64 µg/l (median: 0.33 µg/l) with a maximum of 14.5 µg/l. Maize bran was the principal dairy feed ingredient used by farmers in the sampling, representing more than 65% of the total feed samples collected, with mean aflatoxin concentration of 90.5 µg/kg (median 32.3 µg/kg). The authors note that this preliminary sampling is not generalizable across Rwandan milk production and consumption; the limited pilot study presented here was not designed with the robustness necessary for broad-scale generalization. Thus, the data presented should not be broadly applied outside of the context of the study.