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Preliminary study on the relationship between aflatoxin-bovine serum albumin adducts in blood and aflatoxin M1 levels in milk of dairy cows

Mycotoxin Research volume 36, pages 207–211 (2020)Cite this article

Abstract

The aflatoxin (AF) albumin adduct is often used as a biomarker for aflatoxin exposure in humans. An ELISA method previously used for aflatoxin serum albumin in human blood was used to analyse bovine serum samples (n = 22) collected from dairy cattle during an aflatoxin mitigation study in Kenya. Albumin adduct data were compared with aflatoxin M1 (AFM1) levels in corresponding milk samples from these cows. The concentration ranged from < LOD to 487.9 pg/mL for AFM1 and < LOD and 96.3 pg/mg for aflatoxin-albumin. This study indicates that aflatoxin-albumin adducts could be used as a measure of chronic aflatoxin exposure in dairy cattle.

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Acknowledgements

The authors would like to acknowledge Irene Kagera for her help with the sampling. The authors would like to thank Christopher Wild (IARC, Lyon, France) for providing the aflatoxin-Cl2-BSA polyclonal antibody. It was the essential substance for our study.

Funding

The Kenyan sampling of milk and serum was supported by the Food Africa Programme which is mainly financed by the Ministry for Foreign Affairs of Finland contract no. 29891501 and the CGIAR Research Program on Agriculture for Nutrition and Health.

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Authors and Affiliations

  1. Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Bundesallee 37, D-38116, Brunswick, Germany

    Janine Saltzmann, Susanne Kersten & Sven Dänicke

  2. School of Medicine, University of Leeds, Leeds, UK

    Ya Xu & Michael N. Routledge

  3. School of Food Science and Nutrition, University of Leeds, Leeds, UK

    Yun Yun Gong

  4. International Livestock Research Institute (ILRI), Nairobi, Kenya

    Johanna Lindahl

  5. Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden

    Johanna Lindahl

  6. Department of Microbiology and Immunology, Uppsala University, Uppsala, Sweden

    Johanna Lindahl

  7. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, China

    Michael N. Routledge

Authors
  1. Janine Saltzmann
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  2. Ya Xu
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  3. Yun Yun Gong
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  4. Johanna Lindahl
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  5. Susanne Kersten
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  6. Sven Dänicke
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  7. Michael N. Routledge
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Correspondence to Janine Saltzmann.

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Saltzmann, J., Xu, Y., Gong, Y.Y. et al. Preliminary study on the relationship between aflatoxin-bovine serum albumin adducts in blood and aflatoxin M1 levels in milk of dairy cows. Mycotoxin Res 36, 207–211 (2020). https://doi.org/10.1007/s12550-019-00383-7

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  • DOI: https://doi.org/10.1007/s12550-019-00383-7

Keywords

  • Aflatoxin-albumin adduct
  • Biomarker
  • Aflatoxin M1
  • ELISA
  • Dairy cow