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Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment

A Correction to this article was published on 27 November 2020

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This study aimed to explore involvement of oxidative stress in sterigmatocystin (STC) toxicity in male Wistar rats. Animals were orally treated with a single STC dose (10, 20 and 40 mg/kg b.w.). Short-term treatment resulted in moderate oxidative stress determined by a significant increase of malondialdehyde (MDA; all STC doses) and catalase (CAT; 10 mg/kg b.w.) in plasma, a decrease of glutathione peroxidase (GPx; 20 and 40 mg/kg b.w.) in the liver, and increase of MDA and superoxide dismutase (SOD) in kidneys (all STC doses). Heat shock protein (Hsp27 and Hsp70) expression was determined by Western blotting in rat liver and kidneys. Hsp27 expression was downregulated by STC, particularly in the liver (40 mg/kg b.w.). The lowest STC dose elevated the expression of Hsp70 in both liver and kidneys, while an increase in STC doses restored Hsp70 expression to control. Alterations in expressions of Hsp27 and Hsp70 could be only partially associated with oxidative stress. STC provoked a significant DNA damage in both liver and kidneys (alkaline comet assay), but the liver was more affected by a broader spectrum of DNA lesions. Oxidative DNA damage (hOGG1-modified comet assay) contribute to the overall mechanism of STC-induced DNA damage in both organs, but kidneys in general seem to be more susceptible to oxidative stress upon short-term exposure to sublethal doses of STC.

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  • 27 November 2020

    The original version of this article unfortunately contained a mistake. The author���s first and last names were interchanged in the published version of this article. The corrected author names are given in this article.




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The authors want to thank Vedran Micek, DVM for animal care and performing the treatment and to Mrs. L. Stančin for excellent technical assistance as well as many thanks to Makso Herman, MA for English language editing.


The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was financially supported by the Croatian Science Foundation under project no IP-09-2014-5982 (MycotoxA).

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Correspondence to Šegvić Klarić Maja.

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Dubravka, R., Daniela, J., Andrea, H.T. et al. Sterigmatocystin moderately induces oxidative stress in male Wistar rats after short-term oral treatment. Mycotoxin Res 36, 181–191 (2020).

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  • Sterigmatocystin
  • Malondialdehyde
  • Heat shock proteins
  • DNA damage
  • Comet assay