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Occurrence of relevant mycotoxins in food commodities consumed in Chile

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Abstract

The aims of this study were to analyse the occurrence of aflatoxins, aflatoxin M1 (AFM1), fumonisins, ochratoxin A (OTA), patulin (PAT), zearalenone (ZEN) and deoxynivalenol (DON) in foodstuffs consumed in Chile between 2008 and 2017 and to estimate the contribution of main contaminated foodstuff in human exposure by the probable daily intake (PDI) estimation. In 9 years of surveillance, 2020 food samples were analysed with an occurrence of 18.2% and with 2.7% of the samples being over the Chilean regulation. The occurrence of mycotoxins in food were 16% for aflatoxins, 6% for AFM1, 30% for OTA, 12% for DON, 7% for PAT, 21% for fumonisins and 2% for ZEN. The estimated median PDI of DON because of bread consumption was 129.2 ng/kg bw/day for children and 96.0 ng/kg bw/day in adults. Median PDI because of capsicum consumption was 0.006 ng/kg bw/day for OTA and 0.005 ng/kg bw/day for aflatoxins. Median PDI of aflatoxins was estimated at 0.02 ng/kg bw/day for spices and 0.04 ng/kg bw/day for nuts consumption. In children, the median PDI of AFM1 for dairy consumption was 0.07 ng/kg bw/day. The derived margin of exposure (MoE) values ranged from 1133 to 8500 suggested that aflatoxins would be of public health concern. The PDI of the other mycotoxins did not show a health risk. This is the first survey of mycotoxins in food made in Chile; further research is needed to improve surveillance and guidelines based on national risk assessments and considering sensitive population groups.

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References

  • Ali N, Hashim NH, Shuib NS (2015) Natural occurrence of aflatoxins and ochratoxin A in processed spices marketed in Malaysia. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 32(4):518–532

    CAS  PubMed  Google Scholar 

  • Asai T, Tsuchiya Y, Okano K, Piscoya A, Nishi YC, Ikoma T, Oyama T, Ikegami K, Yamamoto M (2012) Aflatoxin contamination of red chilli pepper from Bolivia and Peru, countries with high gallbladder cancer incidence rates. Asian Pac J Cancer Prev 13:5167–5170

    PubMed  Google Scholar 

  • Barreira MJ, Alvito PC, Almeida CMM (2010) Occurrence of patulin in apple-based foods in Portugal. Food Chem 121:653–658

    CAS  Google Scholar 

  • Benford D (2016) The use of dose-response data in a margin of exposure approach to carcinogenic risk assessment for genotoxic chemicals in food. Mutagenesis 31:329–331

    CAS  PubMed  Google Scholar 

  • Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16(3):497–516

    CAS  PubMed  PubMed Central  Google Scholar 

  • Berdegué F, Jara E, Modrego F, Sanclemente X, Schejtman A (2010) Comunas Rurales de Chile, Documento de Trabajo N° 60. Programa Dinámicas Territoriales Rurales. Rimisp, Santiago

    Google Scholar 

  • Campbell HM, Armstrong JF (2007) Determination of zearalenone in cereal grains, animal feed and feed ingredients using immunoaffinity column chromatography and liquid and chromatography: interlaboratory study. J AOAC Int 90(6):1610–1622

    CAS  PubMed  Google Scholar 

  • Corcuera LA, Arbillaga L, Vettorazzi A, Azqueta A, de Cerain L (2011) Ochratoxin A reduces aflatoxin B1 induced DNA damage detected by the comet assay in Hep G2 cells. Food Chem Toxicol 49:2883–2889

    CAS  PubMed  Google Scholar 

  • Corcuera LA, Vettorazzi A, Arbillaga L, González-Peńas E, López de Cerain A (2012) An approach to the toxicity and toxicokinetics of aflatoxin B1 and ochratoxin A after simultaneous oral administration to fasted F344 rats. Food Chem Toxicol 50:3440–3446

    CAS  PubMed  Google Scholar 

  • Costa J, Rodríguez R, Garcia-Cela E, Medina A, Magan N, Lima N, Battilani P, Santos C (2019) Overview of fungi and mycotoxin contamination in capsicum pepper and in its derivatives. Toxins 11:27

    CAS  PubMed Central  Google Scholar 

  • Degen H, Partosch F, Muñoz K, Gundert-Remy U (2017) Daily uptake of mycotoxins – TDI might not be protective for nursed infants. Toxicol Lett 277:69–75

    CAS  PubMed  Google Scholar 

  • Delgado L (2011) Informe monitoreo de micotoxinas en alimentos. Subsecretaria de Salud Pública- Ministerio de Salud Instituto de Salud Pública de Chile año 2010. http://www.ispch.cl/sites/default/files/documento/2013/05/Informe%20Micotoxinas%202010%20vf.pdf. Accessed 17 July 2019

  • Delgado L (n.d.) Informe monitoreo de micotoxinas en alimentos año 2011b. Subsecretaria de Salud Pública- Ministerio de Salud Instituto de Salud Pública de Chile. http://www.ispch.cl/sites/default/files/documento_tecnico/2012/06/informe_micotoxinas_2011.pdf. Accessed 17 July 2019

  • Delgado L, Villarroel O (n.d.) Micotoxinas en alimentos de consumo directo en Chile. Monitoreo de micotoxinas realizado por el Instituto de Salud Pública de Chile Años 2008-2009. www.ispch.cl/sites/default/files/documento/2013/05/MICOTOXINAS%20EN%20ALIMENTOS%20DE%20CONSUMO%20DIRECTO%20EN%20CHILE%202008-2009.pdf. Accessed 17 July 2019

  • EFSA - European Food Safety Authority (2007) Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to the potential increase of consumer health risk by a possible increase of the existing maximum levels for aflatoxins in almonds, hazelnuts and pistachios and derived products. EFSA J 446:1–127 https://www.efsa.europa.eu/en/efsajournal/pub/446. Accessed 22 June 2019

    Google Scholar 

  • EFSA - European Food Safety Authority (2010) Management of left-censored data in dietary exposure assessment of chemical substances. EFSA J 8(3):1557 https://www.efsa.europa.eu/en/efsajournal/pub/1557. Accessed 22 June 2019

    Google Scholar 

  • EFSA - European Food Safety Authority (2013) International frameworks dealing with human risk assessment of combined exposure to multiple chemicals. EFSA J 11(7):1–69 https://www.efsa.europa.eu/en/efsajournal/pub/3313. Accessed 22 June 2019

    Google Scholar 

  • El Darra N, Gambacorta L, Solfrizzo M (2019) Multimycotoxins occurrence in spices and herbs commercialized in Lebanon. Food Control 95:63–70

    Google Scholar 

  • ENCA (2014) Encuesta de Consumo Alimentario en Chile: Informe final. https://www.minsal.cl/encadescarga/. Accessed 01 December 2018

  • European Commission (2006) Commission Regulation (EC) no 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union L364:1–25 https://eur-lex.europa.eu/legal-content/EN/TXT/?qid=1561986662392&uri=CELEX:32006R1881. Accessed 20 June 2019

    Google Scholar 

  • European Commission (2007) Commission Regulation (EC) no 1126/2007 of 28 September 2007 amending Regulation (EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs as regards Fusarium toxins in maize and maize products. Off J Eur Union L 255/14. https://eur-lex.europa.eu/eli/reg/2007/1126/oj. Accessed 20 June 2019

  • FAO - Food and Agriculture Organization (2004) Worldwide regulations for mycotoxins in food and feed in 2003. FAO food and nutrition paper, 81. Food and Agriculture Organization, Rome. http://www.fao.org/3/y5499e/y5499e00.htm. Accessed 20 June 2019

  • Ferreccio C, Roa JC, Bambs C, Vives A, Corvalán AH, Cortés S, Foerster C, Acevedo J et al (2016) Study protocol for the Maule Cohort (MAUCO) of chronic diseases, Chile 2014-2024. BMC Public Health 16:122

    PubMed  PubMed Central  Google Scholar 

  • Foerster C, Koshiol J, Guerrero AR, Kogan MJ, Ferreccio C (2016) The case for aflatoxins in the causal chain of gallbladder cancer. Med Hypotheses 86:47–52

    CAS  PubMed  Google Scholar 

  • Food Sanitary Regulation of Chile (2017) Reglamento Sanitario de los Alimentos DTO. N° 977/96 (D.OF. 13.05.97) República de Chile Ministerio de Salud. http://www.dinta.cl/wp-dintacl/wp-content/uploads/RSA-DECRETO-977-96-actualizado-25-de-mayo-2017.pdf. Accessed 20 December 2018

  • Franco LT, Petta T, Rottinghaus GE, Bordin K, Gomes GA, Alvito P, Assunção R, Oliveira CAF (2019) Assessment of mycotoxin exposure and risk characterization using occurrence data in foods and urinary biomarkers in Brazil. Food Chem Toxicol 128:21–34

    CAS  PubMed  Google Scholar 

  • Galarce-Bustos O, Alvarado M, Vega M, Aranda M (2014) Occurrence of ochratoxin A in roasted and instant coffees in the Chilean market. Food Control 46:102–107

    CAS  Google Scholar 

  • Gambacorta L, Magistà D, Perrone G, Murgolo S, Logrieco AF, Solfrizzo M (2018) Co-occurrence of toxigenic moulds, aflatoxins, ochratoxin A, Fusarium and Alternaria mycotoxins in fresh sweet peppers (Capsicum annuum) and their processed products. World Mycotoxin J 11(1):159–174

    CAS  Google Scholar 

  • Golli-Bennour EE, Kouidhi B, Bouslimi A, Abid-Essefi S, Hassen W, Bacha H (2010) Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells. J Biochem Mol Toxicol 24:42–50

    PubMed  Google Scholar 

  • IARC- International Agency for Research on Cancer (2012) Sampling and sample preparation methods for determining concentrations of mycotoxins in foods and feeds. IARC Sci Publ 158:39–51 https://www.ncbi.nlm.nih.gov/pubmed/23477195. Accessed 20 June 2019

    Google Scholar 

  • Ikoma T, Tsuchiya Y, Asai T, Okano K, Endoh K, Yamamoto M, Nakamura K (2015) Ochratoxin contamination of red chili peppers from Chile, Bolivia and Peru showing high incidences of gallbladder cancer. Asian Pac J Cancer Prev 16:5897–5991

    Google Scholar 

  • Instituto de Salud Pública de Chile (n.d.) Informe de Resultados de Vigilancia de Laboratorio Micotoxinas en Alimentos. http://www.ispch.cl/sites/default/files/documento/2013/07/Informe%20Micotoxinas%202012%2014-06-2013.pdf. Accessed 20 June 2019

  • Jager AV, Tedesco MP, Souto PCMC, Oliveira CAF (2013) Assessment of aflatoxin intake in São Paulo, Brazil. Food Control 33(1):87–92

    CAS  Google Scholar 

  • Jalili M, Jinap S (2012) Natural occurrence of aflatoxins and ochratoxin A in commercial dried chili. Food Control 24(1):160–164

    CAS  Google Scholar 

  • Jonsyn-Ellis FE (2001) Seasonal variation in exposure frequency and concentration levels of aflatoxins and ochratoxins in urine samples of boys and girls. Mycopathologia 152(1):35–40

    CAS  PubMed  Google Scholar 

  • Karlovsky P, Suman M, Berthiller F, De Meester J, Eisenbrand G, Perrin I et al (2016) Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotoxin Res 32:179–205

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kettler S, Kennedy M, McNamara C, Oberdörfer R, O'Mahony C, Schnabel J, Smith B, Sprong C, Faludi R, Tennant D (2015) Assessing and reporting uncertainties in dietary exposure analysis: mapping of uncertainties in a tiered approach. Food Chem Toxicol 82:79–95

    CAS  PubMed  Google Scholar 

  • Klarić MS, Rašić D, Peraica M (2013) Deleterious effects of mycotoxin combinations involving ochratoxin A. Toxins 5(11):1965–1987

    PubMed  PubMed Central  Google Scholar 

  • Kumagai S, Nakajima M, Tabata S, Ishikuro E, Tanaka T, Norizuki H, Itoh Y, Aoyama K, Fujita K, Kai S, Sato T, Saito S, Yoshiike N, Sugita-Konishi Y (2008) Aflatoxin and ochratoxin A contamination of retail foods and intake of these mycotoxins in Japan. Food Addit Contam Part A 25(9):1101–1106

    CAS  Google Scholar 

  • Marin S, Ramos AJ, Cano-Sancho G, Sanchis V (2013) Mycotoxins: occurrence, toxicology, and exposure assessment. Food Chem Toxicol 60:218–237

    CAS  PubMed  Google Scholar 

  • Muñoz K, Vega M, Rios G, Muñoz S, Madariaga R (2006) Preliminary study of Ochratoxin A in human plasma in agricultural zones of Chile and its relation to food consumption. Food Chem Toxicol 44(11):1884–1889

    PubMed  Google Scholar 

  • Muñoz K, Campos V, Blaszkewic M, Vega M, Alvarez A, Neira J, Degen GH (2010) Exposure of neonates to ochratoxin A: first biomonitoring results in human milk (colostrum) from Chile. Mycotoxin Res 26:59–67

    PubMed  Google Scholar 

  • Muñoz K, Blaszkewicz M, Campos V, Vega M, Degen G (2014) Exposure of infants to ochratoxin A with breast milk. Arch Toxicol 88:837–846

    PubMed  Google Scholar 

  • Muñoz K, Cramer B, Dopstadt J, Humpf HU, Degen GH (2017) Evidence of ochratoxin A conjugates in urine samples from infants and adults. Mycotoxin Res 33(1):39–47

    PubMed  Google Scholar 

  • National Health Survey of Chile (2017). Encuesta Nacional de Salud http://epi.minsal.cl/encuesta-ens/. Accessed 20 December 2018

  • Nogueira L, Foerster C, Groopman J, Egner P, Koshiol J, Ferreccio C (2015) Association of aflatoxin with gallbladder cancer in Chile. JAMA 313:2075–2077

    PubMed  Google Scholar 

  • Ozbey F, Kabak B (2012) Natural co-occurrence of aflatoxins and ochratoxin A in spices. Food Control 28:354–361

    CAS  Google Scholar 

  • Prelle A, Spadaro D, Garibaldi A, Lodovica Gullino M (2014) Co-occurrence of aflatoxins and ochratoxin A in spices commercialized in Italy. Food Control 39(1):192–197

    CAS  Google Scholar 

  • Raiola A, Tenore GC, Manyes L, Meca G, Ritieni A (2015) Risk analysis of main mycotoxins occurring in food for children: an overview. Food Chem Toxicol 84:169–180

    CAS  PubMed  Google Scholar 

  • Renwick AG, Walker R (1993) An analysis of the risk of exceeding the acceptable or tolerable daily intake. Regul Toxicol Pharmacol 18(3):463–480

    CAS  PubMed  Google Scholar 

  • Sedova I, Kiseleva M, Tutelyan V (2018) Mycotoxins in tea: occurrence, methods of determination and risk evaluation. Toxins 10(11):444

    CAS  PubMed Central  Google Scholar 

  • Serra I, Yamamoto M, Calvo A, Cavada G, Báez S, Endoh K, Watanabe H, Tajima K (2002) Association of chili pepper consumption, low socioeconomic status and longstanding gallstones with gallbladder cancer in a Chilean population. Int J Cancer 102:407–411

    CAS  PubMed  Google Scholar 

  • Sherif SO, Salama EE, Abdel-Wahhab MA (2009) Mycotoxins and child health: the need for health risk assessment. Int J Hyg Environ Health 212(4):347–368

    CAS  PubMed  Google Scholar 

  • Sugita-Konsihi Y, Tanaka T, Tabata S, Nakajima M, Nouno M, Nakaie Y, Chonan T, Aoyagi M, Kibune N, Mizuno K, Ishikuro E, Kanamaru N, Minamisawa M, Aita N, Kushiro M, Tanaka K, Takatori K (2006) Validation of an HPLC analytical method coupled to a multifunctional clean-up column for the determination of deoxynivalenol. Mycopathologia 161:239–243

    CAS  PubMed  Google Scholar 

  • Tsuchiya Y, Terao M, Okano K, Nakamura K, Oyama M, Ikegami K et al (2011) Mutagenicity and mutagens of the red chili pepper as gallbladder cancer risk factor in Chilean women. Asian Pac J Cancer Prev 12(2):471–476

    PubMed  Google Scholar 

  • Universidad de Chile (2018) Preliminary study on dairy consumption in Chilean children. http://municipalidadestacioncentral.cl/wp-content/uploads/2018/09/Estudio-de-H%C3%A1bitos-de-Consumo-de-L%C3%A1cteos-en-Ni%C3%B1os-y-Ni%C3%B1as.pdf. Accessed 7 May 2019

  • van Egmond HP, Jonker MA (2004) Worldwide regulations on aflatoxins—the situation in 2002. J Toxicol: Toxin Rev 23(2–3):273–293

    Google Scholar 

  • WHO (2002). Evaluation of certain mycotoxins in food. WHO technical report series, 906

  • WHO - World Health Organization (2006) World Health Organization Child Growth Standards: Methods and development https://www.who.int/childgrowth/standards/technical_report/en/. Accessed 25 February 2019

  • WHO - World Health Organization (2012) Global Environment Monitoring System—Food Contamination Monitoring and Assessment Programme (GEMS/Food) https://www.who.int/nutrition/landscape_analysis/nlis_gem_food/en/. Accessed 30 May 2019

  • WHO - World Health Organization (2017) Evaluations of the Joint FAO/WHO Expert Committee on Food Additives (JECFA) http://apps.who.int/food-additives-contaminants-jecfa-database/search.aspx?fcc=2. Accessed 30 May 2019

  • Yogendrarajah P, Jacxsens L, Lachat C, Walpita CN, Kolsteren P, De Saeger S, De Meulenaer B (2014) Public health risk associated with the co-occurrence of mycotoxins in spices consumed in Sri Lanka. Food Chem Toxicol 74:240–248

    CAS  PubMed  Google Scholar 

  • Zafar Iqbal S, Rafique Asi M, Zuber M, Akhtar J, Jawwad Saif M (2013) Natural occurrence of aflatoxins and ochratoxin A in commercial chilli and chilli sauce samples. Food Control 30(2):621–625

    Google Scholar 

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Acknowledgements

The authors thank the Ministry of Health of Chile for the data used in this paper.

Funding

This study was funded by CONICYT PAI No. 77170040, Universidad de O’Higgins and FONDAP-ACCDIS No. 15130011

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Correspondence to Claudia Foerster.

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Supplementary file S1

Mycotoxins in Chile 2013–2017: Full database (XLSX 72 kb)

Supplementary file S2

Analytic specifications of sample extraction, clean up and Chromatography Methodology (XLSX 5077 kb)

Supplementary file S3

Trends of ochratoxin A and aflatoxin levels and according to the Chilean Regulation (XLSX 23 kb)

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Foerster, C., Muñoz, K., Delgado-Rivera, L. et al. Occurrence of relevant mycotoxins in food commodities consumed in Chile. Mycotoxin Res 36, 63–72 (2020). https://doi.org/10.1007/s12550-019-00369-5

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