Abstract
The present investigations were conducted to test the effects of T-2 toxin on electrophysiological variables of jejunal epithelium of chicken. Jejunal segments of broilers were monitored in Ussing chambers in the presence of T-2 toxin at the levels of 0 (negative control), 0 (methanol/vehicle control), 0.1, 1, 5, and 10 μg/ml of buffer. T-2 toxin did not affect basal values of short circuit current (Isc), transmural potential difference, or tissue conductivity in the jejunal epithelium. T-2 toxin also did not statistically affect glucose-induced electrophysiological variables during the first 3 min of glucose induction. Compared to the vehicle control, the ouabain-sensitive Isc was negatively affected (P = 0.008) only under 5 μg of T-2 toxin/ml. Increasing levels of T-2 toxin negatively affected the ouabain-sensitive Isc in a cubic (P = 0.007) fashion. These data indicate that acute exposure to moderate levels of T-2 toxin may progressively impair the cation gradient across the jejunal epithelium.
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The authors greatly acknowledge the technical assistance by Anett Kriesten and Wageha Ali Awad during the course of the experiments at the Institute of Animal Nutrition, Freie Universität Berlin.
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Yunus, A.W., Kröger, S., Tichy, A. et al. Electrophysiological response of chicken’s jejunal epithelium to increasing levels of T-2 toxin. Mycotoxin Res 29, 23–27 (2013). https://doi.org/10.1007/s12550-012-0142-y
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DOI: https://doi.org/10.1007/s12550-012-0142-y