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Thioredoxin-1 contributes to protection against DON-induced oxidative damage in HepG2 cells

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Abstract

Leucocytes are susceptible to the toxic effects of deoxynivalenol (DON), which is a trichothecene mycotoxin produced by a number of fungi including Fusarium species. One mechanism of action is mediated by reactive oxygen species (ROS). The liver is an important target for toxicity caused by foreign compounds including mycotoxins. On the other hand, little is known about the influence of the redox state on hepatocytes treated with DON. The present study investigated the effect of DON on the cytosolic redox state and antioxidative system in the human hepatoma cell line HepG2. The cell viability of human monocyte cell line THP-1 or leukemia cell line KU812 treated with 2.5 and 5 μmol/l DON were significantly reduced. However, HepG2 cells showed no toxic effects under the same conditions and did not exhibit an increased oxidative state. Further experiments showed that thioredoxin-1 (Trx-1) protein levels but not glutathione increased in the cells treated with 10 μmol/l DON. In addition, the enhancement of Trx-1 content was repressed by antioxidants. These results suggest that DON-induced accumulation of Trx-1 in HepG2 cells plays one of the key roles in protection against cytotoxicity caused by DON and that the mechanism may be mediated by the antioxidant properties of Trx-1.

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Acknowledgements

This work was supported by a Health and Labor Sciences Research Grant from the Ministry of Health, Labor and Welfare of Japan. The authors are also grateful to Rino Yamazaki for her excellent technical assistance.

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Authors and Affiliations

  1. Division of Microbiology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan

    Kei-ichi Sugiyama, Yoichi Kamata & Yoshiko Sugita-Konishi

  2. Department of Applied Biological Chemistry, Faculty of Agriculture, Tamagawa University, 6-1-1 Tamagawagakuen, Machida-shi, Tokyo, 194-8610, Japan

    Mawo Kinoshita & Yuji Minai

  3. Laboratory of Food Environmental Science, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto-shi, Kyoto, 606-8502, Japan

    Fumito Tani

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  1. Kei-ichi Sugiyama
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  2. Mawo Kinoshita
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  3. Yoichi Kamata
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  4. Yuji Minai
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  5. Fumito Tani
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  6. Yoshiko Sugita-Konishi
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Correspondence to Kei-ichi Sugiyama.

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Sugiyama, Ki., Kinoshita, M., Kamata, Y. et al. Thioredoxin-1 contributes to protection against DON-induced oxidative damage in HepG2 cells. Mycotoxin Res 28, 163–168 (2012). https://doi.org/10.1007/s12550-012-0128-9

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  • DOI: https://doi.org/10.1007/s12550-012-0128-9

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