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Evaluation of aflatoxin B1 and ochratoxin A in interacting mixed cultures of Aspergillus sections Flavi and Nigri on peanut grains

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Abstract

The influence of inoculum size on the colony-forming units, production of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was determined when Aspergillus flavus and A. niger aggregate strains were cultured alone and in pairs on irradiated peanut grains at 28°C and 0.97 water activity (aW). The results showed a marked influence of inoculum factor on fungal counts, AFB1 and OTA production in single and paired cultures. Fungal counts of the A. niger aggregate strain in interacting cultures at 7, 14 and 21 days of incubation were significantly higher than those observed in the A. flavus strain, except in the mixed culture with 102 spores/ml of both strains. In all mixed culture assays, the AFB1 production was significantly reduced in comparison with the accumulation of mycotoxin in single cultures. A total inhibition in AFB1 production was observed in some interactions as 102 spores/ml of A. flavus and 103 spores/ml of A. niger aggregate strain at 7 and 14 days, among others. With regard to OTA production, a stimulation in the interacting cultures was observed at all inoculum sizes and incubation period. The highest levels of OTA accumulation were observed at 14 days for all interacting cultures. The maximum level was reach in the culture 103 spores/ml of A. niger aggregate and 104 spores/ml of A. flavus (p < 0.001). These results suggest that, under optimal environmental conditions in peanut grains, the interaction between A. flavus and A. niger aggregate strains could result in an inhibition of AFB1 and in a stimulation of OTA production.

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Acknowledgements

This work was carried out thanks to grants from the Consejo Nacional de Ciencia y Tecnología (CONICET-PIP), Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto (SECYT-UNRC) and Fondo para la Investigación Científica y Tecnológica (FONCYT-PICTO).

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Authors and Affiliations

  1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico, Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional Nº 36 Km 601, 5800, Río Cuarto, Córdoba, Argentina

    Carla L. Barberis, Ana M. Dalcero & Carina E. Magnoli

  2. Fellowship of CONICET, Río Cuarto, Argentina

    Carla L. Barberis

  3. Member of the Research Career of CONICET, Río Cuarto, Argentina

    Ana M. Dalcero & Carina E. Magnoli

Authors
  1. Carla L. Barberis
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  2. Ana M. Dalcero
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  3. Carina E. Magnoli
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Correspondence to Carina E. Magnoli.

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Barberis, C.L., Dalcero, A.M. & Magnoli, C.E. Evaluation of aflatoxin B1 and ochratoxin A in interacting mixed cultures of Aspergillus sections Flavi and Nigri on peanut grains. Mycotoxin Res 28, 149–156 (2012). https://doi.org/10.1007/s12550-012-0126-y

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  • DOI: https://doi.org/10.1007/s12550-012-0126-y

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