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Mycotoxin Research

, Volume 28, Issue 1, pp 17–23 | Cite as

Mycotoxins in South African foods: a case study on aflatoxin M1 in milk

  • Michael Francis DuttonEmail author
  • Mulunda Mwanza
  • Suretha de Kock
  • Lishia Daya Khilosia
Original Paper

Abstract

The contamination of cow’s milk at the farm level with aflatoxin M1 was investigated in South Africa. Samples of feeds, forage, maize and milk were taken at nine dairy farms, and at the same time samples of the processed milk (retail milk) were collected from the respective dairies to which the farms delivered their milk. The feeds were analysed for aflatoxin B1 and the milk samples for aflatoxin M1 using high performance liquid chromatography (HPLC) and fluorescence detection. All milk samples from the dairy farms were positive for aflatoxin M1, ranging from 0.02 μg/l to 1.5 μg/l. Retail milk was also frequently contaminated with AFM1, at levels of 0.01-3.1 μg/l. High AFB1 levels in feed materials on the farms supplying the raw milk indicate that various sources account for this contamination frequency in milk.

Keyword

Fungus Mycotoxin Aflatoxin M1 Milk Biotracer 

Notes

Acknowledgements

Thanks are due to the collaborating dairy and farmers who provided samples of milk, their time and advice, without which this study could not have been done, and also to N. de Villiers for the statistics and the University of Johannesburg for financial support.

Conflicts of interest

None.

References

  1. Commission regulation (EC) No. 466/2001 of 8th March 2001. Official Journal European Communities L 77 16/032000, p 1Google Scholar
  2. Cullen JM, Ruebner BH, Hsieh LS, Hyde DM, Hsieh DPH (1987) Carcinogenicity of dietary aflatoxin M1 in male Fischer rats compared to aflatoxin B1. Cancer Res 47:1913–1917PubMedGoogle Scholar
  3. Dutton MF, Kinsey A (1996) The occurrence of mycotoxins in cereals and animal feedstuffs in Kwazulu Natal, South Africa 1984–1993. S Afr J Anim Sci 26:53–57Google Scholar
  4. Eaton DL, Gallagher EP (1994) Mechanisms of aflatoxin carcinogenesis. Ann Rev Pharmacol Toxicol 34:135–172CrossRefGoogle Scholar
  5. Egal S, Hounsa A, Gong YY, Turner PC, Wild CP, Hall AJ, Hell K, Cardwell KF (2005) Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo West Africa. Internat J Food Microbiol 104:215–224CrossRefGoogle Scholar
  6. Groopman JD, Kensler TW (2005) Role of metabolism and viruses in aflatoxin induced liver cancer. Toxicol Appl Pharmacol 206:131–137PubMedCrossRefGoogle Scholar
  7. Hoorfar J, Wagner M, Jordan K, Boquin S, Skiby J (2011) Towards biotracing in food chains. Internat J Food Microbiol 145(Suppl 1):S1-S4Google Scholar
  8. Hsieh DP (1986) Cancer risks posed by aflatoxin M1, In: Hayashi K (ed) Diet, cancer and nutrition. Japan Science Society, Toyko, pp 56-65Google Scholar
  9. Kang’ethe EK, Lang’a KA (2009) Aflatoxin B1 and M1 contamination of animal feeds and milk from urban centres in Kenya. Afr Health Sci 9:218–226PubMedGoogle Scholar
  10. Manetta AC, Giuseppe LD, Giammarco M, Fusaro I, Simonella A, Gramenzi A, Formigoni A (2005) High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese. J Chromat A 1083:219-222Google Scholar
  11. Naiker D, Marais CJ, van den Berg H, Masango MG (2007) Some fungi, zearalenone and other mycotoxins in chicken rations stock feedstuffs, lucerne and pasture grasses in the communal farming area of Rhenosterkop in South Africa. J S Afr Vet Assoc 78:69–74Google Scholar
  12. Peers FG (1980) Aflatoxin in relation to the epidemiology of human liver cancer. Med Mycol 8:279–289Google Scholar
  13. Rava E, Viljoen JH, Kallmeyer H, de Jager A (1996) Fungi and mycotoxins in South African maize of the 1993 crop. Mycotoxin Research 12:15–29CrossRefGoogle Scholar
  14. Sinha KK, Bhatnagar DP (1998) Mycotoxins in agriculture and food safety. Marcel Dekker, New YorkGoogle Scholar
  15. Skrinjar M, Stubblefield RD, Vujicic IF, Stojanovic E (1992) Distribution of aflatoxin-producing moulds and aflatoxins in dairy cattle feed and raw milk. Acta Microbiol Hungaria 39:174–179Google Scholar
  16. South African Fertilizers Farm Feeds, Agricultural Remedies and Stock Remedies Act 1947 Regulations Relating to Farm Feeds Amendment R227 No. 31958, 2009, p 27Google Scholar
  17. South African Foodstuffs, Cosmetics and Disinfectant Act 1972 (ACT No 54 of 1972) Regulations Governing Tolerance for Fungus-produced Toxins in Foodstuffs Amendment No. 26849, R1145, October 2004, p 1Google Scholar
  18. Trucksess MW, Weaver CM, Oles CJ, Fry FS, Noonan GO, Betz JM, Reader J (2008) Determination of aflatoxin B1, B2, G1, and G2, and ochratoxin A in ginseng and ginger by multitoxin immunoaffinity column cleanup and liquid chromatographic quantitation: collaborative study. J AOAC Int 91:511–523PubMedGoogle Scholar
  19. United States Food and Drug Administration (1996) Sec. 527.400 in FDA Compliance Policy Guidelines. FDA, Washington, p 219Google Scholar
  20. Van Egmond HP (1989) Mycotoxins in dairy products. Elsevier, LondonGoogle Scholar
  21. Yoshikawa H, Uchimaru R, Kamataki T, Kato R, Ueno Y (1982) Metabolism and activation of aflatoxin B1 by reconstituted cytochrome P450 system of rat liver. Cancer Res 42:1120–1124Google Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Michael Francis Dutton
    • 1
    Email author
  • Mulunda Mwanza
    • 1
  • Suretha de Kock
    • 2
  • Lishia Daya Khilosia
    • 1
  1. 1.Food, Environment and Health Research GroupUniversity of JohannesburgDoornfonteinSouth Africa
  2. 2.Department of Food TechnologyUniversity of JohannesburgDoornfonteinSouth Africa

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