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Chemotaxonomic diagnostics: combining sucrose-water agar with TLC to discriminate Fusarium graminearum 3-acetyl-DON and 15-acetyl-DON chemotypes

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Abstract

Twelve randomly-selected isolates of Fusarium graminearum that produce 3-acetyl-deoxynivalenol (3-ADON) or 15-acetyl-deoxynivalenol (15-ADON) were screened by thin-layer chromatography (TLC) for their ability to produce ADON and zearalenone (ZEA) mycotoxins when grown on water agar containing different concentrations of sucrose. The results showed the ability of the F. graminearum 3-ADON chemotype population to produce DON and ZEA at a lower concentration range of sucrose (5-7%) compared with the 15-ADON chemotype (30-40%). The former distinction allows for sucrose-water agar to be employed as a rapid and simple differential medium, where two separate sucrose-gradient concentrations discriminate 3-ADON from 15-ADON populations. In the light of the shift in sugar concentrations occurring during the process of grain formation and maturation, the difference in mycotoxin production between the two populations is discussed with respect to predicting Fusarium head blight (FHB) epidemiology and accumulation of DON and ZEA.

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Acknowledgements

This research was financially supported by Natural Sciences and Engineering Research Council of Canada (NSERC) discovery grant and Saskatchewan Agriculture and Food (SAF)—Agriculture Development Fund (ADF) to V.V. The author would like to thank Dr. Allen (AAFC), Dr. Tekauz (AAFC) and Dr. Ilincic-Tamburic (University of Guelph) for different Fusarium graminearum isolates, P. Daida and Y.K. Goh for technical assistance.

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Authors and Affiliations

  1. Department of Food and Bioproduct Sciences, University of Saskatchewan, Rm 4E22, Agriculture building, 51 Campus Dr, Saskatoon, SK, S7N 5A8, Canada

    Vladimir Vujanovic & Manel Ben Mansour

  2. Department of Plant Biotechnology, University of Tunis El Manar, 1060, Tunis, Tunisia

    Manel Ben Mansour

Authors
  1. Vladimir Vujanovic
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  2. Manel Ben Mansour
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Correspondence to Vladimir Vujanovic.

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Vujanovic, V., Ben Mansour, M. Chemotaxonomic diagnostics: combining sucrose-water agar with TLC to discriminate Fusarium graminearum 3-acetyl-DON and 15-acetyl-DON chemotypes. Mycotoxin Res 27, 295–301 (2011). https://doi.org/10.1007/s12550-011-0107-6

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  • DOI: https://doi.org/10.1007/s12550-011-0107-6

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