Abstract
Deoxynivalenol (DON) is one of the most common Fusarium toxins in animal feed and poses a potential risk especially for monogastric animals like pigs. DON is known to modulate the immune system, dependent on dose and frequency of exposure. The aim of the study was to investigate the effects of chronic exposure to low levels of DON on the expression of immune relevant genes. In a feeding trial (84 days), 20 pigs were assigned equally to a control and a treatment group. The DON-content of the contaminated diet was 1.2 mg/kg from day 1 to 41, from day 42 it was elevated to 2.0 mg/kg. The control group (n = 10) was fed a diet with a DON concentration lower than 0.05 mg/kg. Blood samples were taken over the course of the study and ileum samples were taken at slaughter. Gene expression measurement was done using real-time RT-qPCR. For target genes, those cytokines were chosen, which were estimated to be implicated in the modulation of the immune system induced by DON ingestion. In ileum, significant down-regulations could be observed for IL-1β and IL-8 (p < 0.05). Most significant regulations in blood could be detected on day 45 after increasing the dietary DON content in the experimental diet. Herein, down-regulations of IL-1β, IL-8 and TNFα were demonstrated. In conclusion, the present study provides data concerning chronic application of DON in low doses, as little is known in this area. Down-regulations of immune-related transcription factors and pro-inflammatory immune factors could be demonstrated.
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Accensi F, Pinton P, Callu P, bella-Bourges N, Guelfi JF, Grosjean F, Oswald IP (2006) Ingestion of low doses of deoxynivalenol does not affect hematological, biochemical, or immune responses of piglets. J Anim Sci 84:1935–1942
Becker C, Hammerle-Fickinger A, Riedmaier I, Pfaffl MW (2010) mRNA and microRNA quality control for RT-qPCR analysis. Methods 50:237–243
Bergsjo B, Langseth W, Nafstad I, Jansen JH, Larsen HJ (1993) The effects of naturally deoxynivalenol-contaminated oats on the clinical condition, blood parameters, performance and carcass composition of growing pigs. Vet Res Commun 17:283–294
Cunningham-Rundles S, McNeeley DF, Moon A (2005) Mechanisms of nutrient modulation of the immune response. J Allergy Clin Immunol 115:1119–1128
Danicke S, Valenta H, Klobasa F, Doll S, Ganter M, Flachowsky G (2004) Effects of graded levels of Fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrient digestibility, deoxynivalenol balance and clinical serum characteristics. Arch Anim Nutr 58:1–17
Doll S, Danicke S, Ueberschar KH, Valenta H, Schnurrbusch U, Ganter M, Klobasa F, Flachowsky G (2003) Effects of graded levels of Fusarium toxin contaminated maize in diets for female weaned piglets. Arch Tierernahr 57:311–334
Drochner W, Schollenberger M, Piepho HP, Gotz S, Lauber U, Tafaj M, Klobasa F, Weiler U, Claus R, Steffl M (2004) Serum IgA-promoting effects induced by feed loads containing isolated deoxynivalenol (DON) in growing piglets. J Toxicol Environ Health A 67:1051–1067
Eriksen GS, Pettersson H, Lindberg JE (2003) Absorption, metabolism and excretion of 3-acetyl DON in pigs. Arch Tierernahr 57:335–345
Fleige S, Preissinger W, Meyer HH, Pfaffl MW (2009) The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves. J Anim Sci 87:1731–1738
Goyarts T, Danicke S, Rothkotter HJ, Spilke J, Tiemann U, Schollenberger M (2005) On the effects of a chronic deoxynivalenol intoxication on performance, haematological and serum parameters of pigs when diets are offered either for ad libitum consumption or fed restrictively. J Vet Med A Physiol Pathol Clin Med 52:305–314
Goyarts T, Danicke S, Tiemann U, Rothkotter HJ (2006) Effect of the Fusarium toxin deoxynivalenol (DON) on IgA IgM and IgG concentrations and proliferation of porcine blood lymphocytes. Toxicol In Vitro 20:858–867
Larsen JC, Hunt J, Perrin I, Ruckenbauer P (2004) Workshop on trichothecenes with a focus on DON: summary report. Toxicol Lett 153:1–22
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25:402–408
Pestka JJ (2010) Deoxynivalenol: mechanisms of action, human exposure, and toxicological relevance. Arch Toxicol 84:663–679
Pestka JJ ,Bondy GS (1994) Mycotoxin-induced immune modulation. In: Dean JH, Luser MI, Munson AE, and Kimber I (eds) Immunotoxicology and immunopharmacology, 2nd edn. Raven Press, New York
Pinton P, Accensi F, Beauchamp E, Cossalter AM, Callu P, Grosjean F, Oswald IP (2008) Ingestion of deoxynivalenol (DON) contaminated feed alters the pig vaccinal immune responses. Toxicol Lett 177:215–222
Prelusky DB, Gerdes RG, Underhill KL, Rotter BA, Jui PY, Trenholm HL (1994) Effects of low-level dietary deoxynivalenol on haematological and clinical parameters of the pig. Nat Toxins 2:97–104
Rocha O, Ansari K, Doohan FM (2005) Effects of trichothecene mycotoxins on eukaryotic cells: a review. Food Addit Contam 22:369–378
Rotter BA, Thompson BK, Lessard M, Trenholm HL, Tryphonas H (1994) Influence of low-level exposure to Fusarium mycotoxins on selected immunological and hematological parameters in young swine. Fundam Appl Toxicol 23:117–124
Rotter BA, Prelusky DB, Pestka JJ (1996) Toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health 48:1–34
Schedle K, Pfaffl MW, Plitzner C, Meyer HH, Windisch W (2008) Effect of insoluble fibre on intestinal morphology and mRNA expression pattern of inflammatory, cell cycle and growth marker genes in a piglet model. Arch Anim Nutr 62:427–438
Swamy HV, Smith TK, MacDonald EJ, Karrow NA, Woodward B, Boermans HJ (2003) Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on growth and immunological measurements of starter pigs, and the efficacy of a polymeric glucomannan mycotoxin adsorbent. J Anim Sci 81:2792–2803
Yan D, Zhou HR, Brooks KH, Pestka JJ (1997) Potential role for IL-5 and IL-6 in enhanced IgA secretion by Peyer’s patch cells isolated from mice acutely exposed to vomitoxin. Toxicology 122:145–158
Zcona-Olivera JI, Ouyang Y, Murtha J, Chu FS, Pestka JJ (1995) Induction of cytokine mRNAs in mice after oral exposure to the trichothecene vomitoxin (deoxynivalenol): relationship to toxin distribution and protein synthesis inhibition. Toxicol Appl Pharmacol 133:109–120
Zhou HR, Yan D, Pestka JJ (1997) Differential cytokine mRNA expression in mice after oral exposure to the trichothecene vomitoxin (deoxynivalenol): dose response and time course. Toxicol Appl Pharmacol 144:294–305
Zhou HR, Islam Z, Pestka JJ (2003) Rapid, sequential activation of mitogen-activated protein kinases and transcription factors precedes proinflammatory cytokine mRNA expression in spleens of mice exposed to the trichothecene vomitoxin. Toxicol Sci 72:130–142
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Becker, C., Reiter, M., Pfaffl, M.W. et al. Expression of immune relevant genes in pigs under the influence of low doses of deoxynivalenol (DON). Mycotoxin Res 27, 287–293 (2011). https://doi.org/10.1007/s12550-011-0106-7
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DOI: https://doi.org/10.1007/s12550-011-0106-7