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Fabrication of detachable hydrogel microplates for separably patterned cell culture

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Abstract

The well plates allow the reduced consumption of culture media in the conventional cell culture. Combined with microtechnology, microengineered templates allowed a precise control of the size, shape and location of the cultured cell into micropatterns. The localized, and micropatterned culture provides many number of cell samples for the subsequent analysis which are cultured at the same condition. However the patterned cells are immobilized on the surface, therefore the limitation still exists that there are difficulties on the cell analysis of the separated cell patterns. In this study, we propose a fabrication method of separable type Poly ethylene glycol diacrylate (PEGDA) microplates and its application onto a micropatterned culture of NIH/3T3 cells. PEGDA microplates were fabricated by single process of photholithography of hydrogel. Collagen layer was transferred onto PEGDA microplates to improve cell-microplate adhesion using μCP method. The 400 μm dot patterns were used in the test. The fabrication process is very simple, low-cost, and does not require expensive MEMS facilities. Because the fabricated microplates are detachable, it provides efficient way for further studies of cell biology by allowing separated analysis of many number of cell samples which are cultured at the same environment with small amount of cell media.

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Authors and Affiliations

  1. School of Mechanical Engineering, Kyungpook National University, #1370 Sangyuk-Dong, Buk-gu, Daegu, 702-701, South Korea

    Jin Ho Choi, Cheol Woo Park & Gyu Man Kim

  2. Department of Laboratory Animal Medicine, Cell and Matrix Research Institute, College of Veterinary Medicine, Kyungpook National University, #1370 Sangyuk-Dong, Buk-gu, Daegu, 702-701, South Korea

    Hee Kyung Jin

  3. Department of Physiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Jung-Gu, Daegu, 700-842, South Korea

    Jae-Sung Bae

  4. Department of Applied Chemistry, Kyungpook National University, #1370 Sangyuk-Dong, Buk-gu, Daegu, 702-701, South Korea

    In Woo Cheong

Authors
  1. Jin Ho Choi
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  2. Hee Kyung Jin
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  3. Jae-Sung Bae
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  4. Cheol Woo Park
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  5. In Woo Cheong
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  6. Gyu Man Kim
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Correspondence to Gyu Man Kim.

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Choi, J.H., Jin, H.K., Bae, JS. et al. Fabrication of detachable hydrogel microplates for separably patterned cell culture. Int. J. Precis. Eng. Manuf. 15, 945–948 (2014). https://doi.org/10.1007/s12541-014-0421-y

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  • DOI: https://doi.org/10.1007/s12541-014-0421-y

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