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Design, Synthesis, Spectral Analysis, In Vitro Anticancer Evaluation and Molecular Docking Studies of Some Fluorescent 4-Amino-2, 3-Dimethyl-1-Phenyl-3-Pyrazolin-5-One, Ampyrone Derivatives

  • D. PremnathEmail author
  • Israel V. M. V. Enoch
  • P. Mosae Selvakumar
  • M. Indiraleka
  • J. Jannet Vennila
Original Research Article

Abstract

The commenced work deals with the synthesis, characterization and evaluation of biological activities of 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one. The synthesis was done by the condensation of aromatic acid chlorides with 4-aminoantipyrine. The structures of synthesized derivatives were elucidated using IR, Mass, 1H NMR and 13C NMR spectroscopy, and their UV–Visible and fluorescence properties were studied. The compounds showed significant dual fluorescence. Molecular docking was used to understand the small molecule–receptor protein interaction. The derivatives were screened for their in vitro cytotoxic activity against the reference drug pazopanib on human cervical cancer cell line (SiHa) using MTT assay.

Keywords

4-Aminoantipyrine Fluorescence spectroscopy Molecular docking Cytotoxic activity 

Notes

Acknowledgments

The authors thank the management and the authorities of Karunya University, Coimbatore, for their kind support, constant encouragement, and providing laboratory facility. We extend our thanks to SAIF, IIT, Madras, India, and IISC, Bangalore, India, for recording NMR and mass spectra.

Compliance with Ethical Standards

Conflict of interests

The authors declare that there is no conflict of interests.

Supplementary material

12539_2015_138_MOESM1_ESM.doc (1.5 mb)
Supplementary material 1 (DOC 1539 kb)

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Copyright information

© International Association of Scientists in the Interdisciplinary Areas and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • D. Premnath
    • 1
    Email author
  • Israel V. M. V. Enoch
    • 2
  • P. Mosae Selvakumar
    • 2
  • M. Indiraleka
    • 3
  • J. Jannet Vennila
    • 1
  1. 1.Department of Biotechnology, School of Health Science and BiotechnologyKarunya UniversityCoimbatoreIndia
  2. 2.Department of Chemistry, School of Science and HumanitiesKarunya UniversityCoimbatoreIndia
  3. 3.Department of BiotechnologyMepco Schlenk Engineering CollegeSivakasiIndia

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