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Design, Synthesis, Spectral Analysis, In Vitro Anticancer Evaluation and Molecular Docking Studies of Some Fluorescent 4-Amino-2, 3-Dimethyl-1-Phenyl-3-Pyrazolin-5-One, Ampyrone Derivatives

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Abstract

The commenced work deals with the synthesis, characterization and evaluation of biological activities of 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one. The synthesis was done by the condensation of aromatic acid chlorides with 4-aminoantipyrine. The structures of synthesized derivatives were elucidated using IR, Mass, 1H NMR and 13C NMR spectroscopy, and their UV–Visible and fluorescence properties were studied. The compounds showed significant dual fluorescence. Molecular docking was used to understand the small molecule–receptor protein interaction. The derivatives were screened for their in vitro cytotoxic activity against the reference drug pazopanib on human cervical cancer cell line (SiHa) using MTT assay.

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Acknowledgments

The authors thank the management and the authorities of Karunya University, Coimbatore, for their kind support, constant encouragement, and providing laboratory facility. We extend our thanks to SAIF, IIT, Madras, India, and IISC, Bangalore, India, for recording NMR and mass spectra.

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Authors and Affiliations

  1. Department of Biotechnology, School of Health Science and Biotechnology, Karunya University, Coimbatore, Tamil Nadu, 641114, India

    D. Premnath & J. Jannet Vennila

  2. Department of Chemistry, School of Science and Humanities, Karunya University, Coimbatore, Tamil Nadu, 641114, India

    Israel V. M. V. Enoch & P. Mosae Selvakumar

  3. Department of Biotechnology, Mepco Schlenk Engineering College, Sivakasi, Tamil Nadu, India

    M. Indiraleka

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  1. D. Premnath
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  2. Israel V. M. V. Enoch
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  3. P. Mosae Selvakumar
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  4. M. Indiraleka
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  5. J. Jannet Vennila
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Correspondence to D. Premnath.

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Premnath, D., Enoch, I.V.M.V., Selvakumar, P.M. et al. Design, Synthesis, Spectral Analysis, In Vitro Anticancer Evaluation and Molecular Docking Studies of Some Fluorescent 4-Amino-2, 3-Dimethyl-1-Phenyl-3-Pyrazolin-5-One, Ampyrone Derivatives. Interdiscip Sci Comput Life Sci 9, 130–139 (2017). https://doi.org/10.1007/s12539-015-0138-3

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  • DOI: https://doi.org/10.1007/s12539-015-0138-3

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