Computational Identification of MicroRNAs and Their Targets from Finger Millet (Eleusine coracana)

  • S. Usha
  • M. N. Jyothi
  • B. Suchithra
  • Rekha Dixit
  • D. V. Rai
  • R. Nagesh babuEmail author
Original Research Article


MicroRNAs are endogenous small RNAs regulating intrinsic normal growth and development of plant. Discovering miRNAs, their targets and further inferring their functions had become routine process to comprehend the normal biological processes of miRNAs and their roles in plant development. In this study, we used homology-based analysis with available expressed sequence tag of finger millet (Eleusine coracana) to predict conserved miRNAs. Three potent miRNAs targeting 88 genes were identified. The newly identified miRNAs were found to be homologous with miR166 and miR1310. The targets recognized were transcription factors and enzymes, and GO analysis showed these miRNAs played varied roles in gene regulation. The identification of miRNAs and their targets is anticipated to hasten the pace of key epigenetic regulators in plant development.


EST GO analysis MYB Transcription factors 



This work is supported by Department of Science and Technology (SR/FT/LS-10/2012), New Delhi, India.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Supplementary material

12539_2015_130_MOESM1_ESM.xlsx (10 kb)
Supplementary file 1 Precursor sequences of miRNAs in Finger millet (xlsx 9 kb)
12539_2015_130_MOESM2_ESM.xlsx (14 kb)
Supplementary file 2 GO terms for targets identified in Finger millet (xlsx 14 kb)


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Copyright information

© International Association of Scientists in the Interdisciplinary Areas and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • S. Usha
    • 1
    • 2
  • M. N. Jyothi
    • 1
    • 2
  • B. Suchithra
    • 1
  • Rekha Dixit
    • 2
  • D. V. Rai
    • 2
  • R. Nagesh babu
    • 1
    Email author
  1. 1.Maharani’s Science College for WomenBangaloreIndia
  2. 2.Faculty of Biological EngineeringShobhit UniversityMeerutIndia

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