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Computational Identification of MicroRNAs and Their Targets from Finger Millet (Eleusine coracana)

Interdisciplinary Sciences: Computational Life Sciences volume 9, pages 72–79 (2017)Cite this article

Abstract

MicroRNAs are endogenous small RNAs regulating intrinsic normal growth and development of plant. Discovering miRNAs, their targets and further inferring their functions had become routine process to comprehend the normal biological processes of miRNAs and their roles in plant development. In this study, we used homology-based analysis with available expressed sequence tag of finger millet (Eleusine coracana) to predict conserved miRNAs. Three potent miRNAs targeting 88 genes were identified. The newly identified miRNAs were found to be homologous with miR166 and miR1310. The targets recognized were transcription factors and enzymes, and GO analysis showed these miRNAs played varied roles in gene regulation. The identification of miRNAs and their targets is anticipated to hasten the pace of key epigenetic regulators in plant development.

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Acknowledgments

This work is supported by Department of Science and Technology (SR/FT/LS-10/2012), New Delhi, India.

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Affiliations

  1. Maharani’s Science College for Women, Palace road, Bangalore, 560001, India

    S. Usha, M. N. Jyothi, B. Suchithra & R. Nagesh babu

  2. Faculty of Biological Engineering, Shobhit University, Meerut, UP, 250110, India

    S. Usha, M. N. Jyothi, Rekha Dixit & D. V. Rai

Authors
  1. S. Usha
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  2. M. N. Jyothi
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  3. B. Suchithra
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  4. Rekha Dixit
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  5. D. V. Rai
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  6. R. Nagesh babu
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Correspondence to R. Nagesh babu.

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The authors declare that they have no conflict of interest. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Electronic supplementary material

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Supplementary file 1

Precursor sequences of miRNAs in Finger millet (xlsx 9 kb)

Supplementary file 2

GO terms for targets identified in Finger millet (xlsx 14 kb)

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Usha, S., Jyothi, M.N., Suchithra, B. et al. Computational Identification of MicroRNAs and Their Targets from Finger Millet (Eleusine coracana). Interdiscip Sci Comput Life Sci 9, 72–79 (2017). https://doi.org/10.1007/s12539-015-0130-y

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  • DOI: https://doi.org/10.1007/s12539-015-0130-y

Keywords

  • EST
  • GO analysis
  • MYB
  • Transcription factors