Resolving the ambiguities in the identification of two smooth-hound sharks (Mustelus mustelus and Mustelus punctulatus) using genetics and morphology

  • I. A. M. Marino
  • L. Finotto
  • F. Colloca
  • M. Di Lorenzo
  • M. Gristina
  • E. D. Farrell
  • L. Zane
  • C. Mazzoldi
Original Paper

Abstract

Elasmobranch species are among the most threatened marine fish, and sound biological data for many of them are lacking. In this context, the smooth-hounds (Mustelus spp., Triakidae, Carcharhiniformes) represent an intriguing genus, being characterized by unclear and sometimes contrasting diagnostic traits. Here, we present new insights into the identification of two closely related species, M. mustelus and M. punctulatus. The use of three different molecular markers on a dataset of 588 specimens highlighted a genetic distinction between the two species. However, whereas microsatellites provided unambiguous results in all the samples, the other two markers were not able to assign a fraction of the individuals (6.6% for cytochrome c oxidase subunit 1, 14.4% for Internal Transcribed Spacer 2), suggesting the occurrence of heteroplasmy and introgression due to past hybridisation events. The comparison between morphological traits, previously suggested as diagnostic for species identification, and genetic identification, enabled validation of the most reliable and practical morphological traits for species identification. The shape of the dermal denticles represents the most reliable trait, but its use during fieldwork may be impractical. The black spots on body sides were present almost exclusively in M. punctulatus, allowing its identification; however, their absence is not diagnostic for M. mustelus. The distance of the nostrils and the shape of the mouth are useful to distinguish the two species during fieldwork. Our results will allow an accurate identification of the two species leading to the collection of reliable data on their distribution, life history traits, exploitation and status of conservation.

Keywords

Morphological identification Genetic identification Microsatellites COI; Triakidae Smooth-hound shark 

Supplementary material

12526_2017_701_MOESM1_ESM.pdf (628 kb)
ESM 1(PDF 628 kb)
12526_2017_701_MOESM2_ESM.xlsx (275 kb)
ESM 2(XLSX 274 kb)

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Copyright information

© Senckenberg Gesellschaft für Naturforschung and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of BiologyUniversity of PadovaPadovaItaly
  2. 2.IAMC-CNRMazara del ValloItaly
  3. 3.School of Biology & Environmental Science, Science Centre WestUniversity College DublinBelfieldIreland
  4. 4.Consorzio Nazionale Interuniversitario per le Scienze del Mare (CoNISMa)RomeItaly

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