Molecular identification and systematic assessment of Labidocera madurae A. Scott, 1909 (calanoid copepod) from Lakshadweep Archipelago, southwest coast of India, based on mitochondrial COI gene sequences

Abstract

Labidocera madurae A. Scott, 1909, belonging to the family Pontellidae, may easily be mistaken for Labidocera pavo Giesbrecht 1889 or Labidocera bataviae A. Scott, 1909. L. madurae is distinguishable from its congeners by the presence of a moderately large and asymmetrical genital segment, with the middle of the right side considerably inflated and with a short anal segment in females, and a short, curved, thumb-like process on the proximal outer margin of the first joint of the right exopodite of the fifth pair of feet, the apical joint of left exopodite elongate and ovate, and twice as long as broad in males. Since Labidocera individuals exhibit close proximity in morphology, there exists the potential for misidentification. Intraspecific distance of L. madurae sequences developed in the present study ranged from 0.2 to 0.9 %, which was adequate to confirm the genetic congruence of the same. This study also supported the differentiation of Labidocera madurae from other individuals of the genus Labidocera. A point of major confusion concerning L. madurae and Labidocera sp. from the Hawaiian region was resolved in this study, as is evident from the molecular inference that L. madurae and Labidocera sp. individuals were the descendants of a common ancestor and close relatives to other individuals within the genus. The present database represents the first molecular data for L. madurae, which will be adaptable for resolving taxonomic ambiguities and inferring phylogenetic relationships of the genus Labidocera.

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Acknowledgments

The authors wish to thank the Department of Biotechnology, Government of India, for providing financial support, and the Head of the Department of Marine Biology, Microbiology and Biochemistry, School of Marine Science, Cochin University of Science and Technology for providing the necessary facilities to carry out the work. We are especially grateful for the assistance rendered by the Department of Science and Technology, Union Territory of Lakshadweep, in granting research permission and providing other logistic support.

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Correspondence to S. Bijoy Nandan.

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Communicated by P. Martinez Arbizu

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Sanu, V.F., Nandan, S.B., Deepak, J. et al. Molecular identification and systematic assessment of Labidocera madurae A. Scott, 1909 (calanoid copepod) from Lakshadweep Archipelago, southwest coast of India, based on mitochondrial COI gene sequences. Mar Biodiv 46, 95–103 (2016). https://doi.org/10.1007/s12526-015-0326-8

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Keywords

  • Labidocera madurae
  • DNA barcoding
  • Lakshadweep
  • Calanoid copepods