Abstract
Purpose
We explored the possibility of employing intracytoplasmic sperm injection (ICSI), involving oocytes and sperm of owl monkeys, to increase the availability of this species for investigations relating to malaria, etc., by increasing the number of animals in our laboratory.
Methods
Two owl monkeys (a female and a male), raised at the Amami Laboratory of the University of Tokyo, were used. Follicular oocytes surrounded with cumulus cells were cultured in vitro for approximately 25 h and cumulus cells were removed with 0.1 % hyaluronidase. Because of the poor motility of caudal epididymal sperm, sperm were injected without adding polyvinylpyrrolidone to immobilize them. The ICSI procedure was performed by an individual with considerable experience of human ICSI.
Results
We were able to produce two owl monkey embryos using ICSI of oocytes that matured to MII stage. Both embryos reached the 10-cell stage at 98 h after ICSI and showed signs of compaction, but failed to cleave further.
Conclusions
Although we successfully produced owl monkey embryos after ICSI, the embryos did not develop to the blastocyst stage. Many parameters need to be studied further, including superovulation, selection of culture media, and selection of good quality sperm in order to achieve successful ICSI in the owl monkey.
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Acknowledgments
We thank Emeritus Professor Lynn R. Fraser of King’s College London for reading this manuscript. This study was supported partly by the Grant for Joint Research Project (2013-328) of the Institute of Medical Science, The University of Tokyo.
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This article does not contain any studies with human subjects performed by any of the authors.
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Watanabe, H., Matsumoto, T., Nishi, M. et al. Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey (Aotus lemurinus). Reprod Med Biol 15, 183–186 (2016). https://doi.org/10.1007/s12522-015-0229-1
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DOI: https://doi.org/10.1007/s12522-015-0229-1