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Successful delivery following intracytoplasmic sperm injection with calcium ionophore A23187 oocyte activation in a partially globozoospermic patient

  • Case Report
  • Published:
Reproductive Medicine and Biology

Abstract

Purpose

To describe a successful pregnancy outcome following intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA) in a case of partial globozoospermia.

Methods

AOA was accomplished with calcium ionophore A23187. Sperm morphology was observed via light, fluorescent and electron microscopy following a Diff-Quik stain and fluorescein isothiocyanate-labeled peanut agglutinin (FITC-PNA) staining. An activation ability test was employed using a mouse oocyte exposed to strontium chloride.

Results

Via light microscopy, it was found that a large number of sperm possessed deficient acrosomes and a sharply rounded head; however, we observed both normal and the aforementioned abnormal sperm via FITC-PNA staining of a semen specimen. Mouse oocyte activation was 87.5 % via natural activation without AOA. With AOA after ICSI, 100 % oocyte activation was observed. Five oocytes were retrieved, and AOA with A23187 after ICSI resulted in a high fertilization rate (4 of 5, 80 %). Two embryos developed and the patient subsequently delivered a healthy female infant without any congenital abnormalities.

Conclusions

We report a successful pregnancy outcome using an early stage embryo, which developed following ICSI using sperm from a partially globozoospermic patient who possessed temporary potential oocyte activation.

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Authors and Affiliations

  1. Shiba Park Kamiyama Clinic, Daiyu-BL, 2-9-10 Shiba, Minato-ku, Tokyo, 105-0014, Japan

    Hiroshi Kamiyama, Taketoshi Shimizu, Tomomi Oki & Terumi Asada

  2. The Institute for Advanced Reproductive Medical Technology, 909-21 Ishii, Fujimi, Maebashi, Gunma, 371-0105, Japan

    Yasuyuki Araki & Yasuhisa Araki

Authors
  1. Hiroshi Kamiyama
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  2. Taketoshi Shimizu
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  4. Terumi Asada
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  5. Yasuyuki Araki
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  6. Yasuhisa Araki
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Correspondence to Hiroshi Kamiyama.

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Kamiyama, H., Shimizu, T., Oki, T. et al. Successful delivery following intracytoplasmic sperm injection with calcium ionophore A23187 oocyte activation in a partially globozoospermic patient. Reprod Med Biol 11, 159–164 (2012). https://doi.org/10.1007/s12522-012-0123-z

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  • DOI: https://doi.org/10.1007/s12522-012-0123-z

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