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The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes

  • Original Article
  • Published:
Reproductive Medicine and Biology

Abstract

Purpose

This study was conducted to investigate the effects of supplementing fructose in the culture medium on in vitro maturation (IVM), in vitro fertilization (IVF), and metabolism of porcine oocytes.

Methods

Porcine oocytes were matured in vitro in modified North Carolina State University 37 medium (NCSU-37) and then supplemented with either glucose (5.5 mM), fructose (5.5 mM), or glucose (2.75 mM) plus fructose (2.75 mM). The maturation and fertilization of oocytes, and the incorporation and oxidation of 14C-glucose, 14C-fructose, and 14C-methionine in oocytes at different stages of development were examined.

Results

The supplementation of glucose plus fructose significantly promoted (P < 0.05) oocytes germinal vesicle break down (GVBD), maturation to metaphase II (MII), penetration by spermatozoa, and male pronuclear formation compared with glucose. The incorporation and oxidation of 14C-methionine into the oocyte significantly increased (P < 0.05) with glucose plus fructose supplementation than glucose. A significantly higher (P < 0.05) rate of incorporation and oxidation was achieved with 14C-fructose compared to 14C-glucose.

Conclusions

Glucose plus fructose supplementation improved maturation, penetration by spermatozoa, male pronuclear formation, and energy metabolism by porcine oocytes.

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References

  1. Beckmann LS, Day BN. Effects of media NaCl concentration and osmolarity on the culture of early-stage pig embryos and the viability of embryos cultured in a selected superior medium. Theriogenology. 1993;39:611–22.

    Article  PubMed  CAS  Google Scholar 

  2. Petters RM, Reed ML. Addition of taurine or hypotaurine to culture medium improves development of one and two cell pig embryos in vitro. Theriogenology. 1991;35:253.

    Article  Google Scholar 

  3. Petters RM, Wells KD. Culture of pig embryos. J Reprod Fertil. 1993;48:61–73.

    CAS  Google Scholar 

  4. Youngs CR, Ford SP, McGinnis LK, Anderson LH. Investigations into the control of litter size in swine: I. Comparative studies on in vitro development of Meishan and Yorkshire preimplantation embryos. J Anim Sci. 1993;71:1561–5.

    PubMed  CAS  Google Scholar 

  5. Dobrinsky JR, Johnson LA, Rath D. Development of a culture medium (BECM-3) for porcine embryos: effect of bovine serum albumin and bovine serum on embryo development. Biol Reprod. 1996;55:1069–74.

    Article  PubMed  CAS  Google Scholar 

  6. Kikuchi K. Developmental competence of porcine blastocysts produced in vitro. J Reprod Dev. 2004;50:21–8.

    Article  PubMed  Google Scholar 

  7. Machaty Z, Day BN, Prather RS. Development of early porcine embryos in vitro and in vivo. Biol Reprod. 1998;59:451–5.

    Article  PubMed  CAS  Google Scholar 

  8. Kim JH, Niwa K, Lim JM, Okuda K. Effects of phosphate, energy substrates and amino acids on development of in vitro-matured, in vitro-fertilized bovine oocytes in a chemically defined, protein-free culture medium. Biol Reprod. 1993;48:1320–5.

    Article  PubMed  CAS  Google Scholar 

  9. Khurana NK, Niemann H. Effects of cryopreservation on glucose metabolism and survival of bovine morulae and blastocysts derived in vitro or in vivo. Theriogenology. 2000;54:313–26.

    Article  PubMed  CAS  Google Scholar 

  10. Gregorie AT, Gibbon R. Glucosyl oligosaccharides of the rabbit genital tract: effects of ovarian hormone administration. Int J Fertil. 1965;10:151–7.

    Google Scholar 

  11. Suga T, Masaki J. Studies on the secretions of the cow. 6. Sugar and polyol constituents in the luminal fluid of the bovine uterus. Jpn J Anim Reprod. 1973;18:143–7.

    Google Scholar 

  12. Ludwig TE, Lane M, Bavister BD. Differential effect of hexoses on hamster embryo development in culture. Biol Reprod. 2001;64:1366–74.

    Article  PubMed  CAS  Google Scholar 

  13. Kwun J, Chang K, Lim J, et al. Effects of exogenous hexoses on bovine in vitro fertilized and cloned embryo development: improved blastocyst formation after glucose replacement with fructose in a serum-free culture medium. Mol Reprod Dev. 2003;65:167–74.

    Article  PubMed  CAS  Google Scholar 

  14. Guyader-Joly C, Khatchadourian C, Menezo Y. Comparative glucose and fructose incorporation and conversion by in vitro produced bovine embryos. Zygote. 1996;4:85–91.

    Article  PubMed  CAS  Google Scholar 

  15. Sakkas D, Urner F, Menezo Y, Leppens G. Effects of glucose and fructose on fertilization, cleavage, and viability of mouse embryos in vitro. Biol Reprod. 1993;49:1288–92.

    Article  PubMed  CAS  Google Scholar 

  16. Wongsrikeao P, Otoi T, Taniguchi M, et al. Effects of hexoses on in vitro oocyte maturation and embryo development in pigs. Theriogenology. 2006;65:332–43.

    Article  PubMed  CAS  Google Scholar 

  17. Hwang WS, Ryu YJ, Park JH, et al. Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst. Science. 2004;303:1669–74.

    Article  PubMed  CAS  Google Scholar 

  18. Augustin R, Pocar P, Navarrete-Santos A, et al. Glucose transporter expression is developmentally regulated in vitro derived bovine preimplantation embryos. Mol Reprod Dev. 2001;60:370–6.

    Article  PubMed  CAS  Google Scholar 

  19. Tsujii H, Ohta E, Miah AG, Hossain MS, Salma U. Effect of fructose on motility, acrosome reaction, and in vitro fertilization capability of boar spermatozoa. Reprod Med Biol. 2006;5:255–61.

    Article  CAS  Google Scholar 

  20. Parrish JJ, Susko-Parrish J, Winer MA, First NL. Capacitation of bovine sperm by heparin. Biol Reprod. 1988;38:1171–80.

    Article  PubMed  CAS  Google Scholar 

  21. Tsujii H, Nakamura Y, Hamano K. In vitro effects of insulin on glucose and lipid metabolism in rat embryos. Anim Sci J. 2002;73:185–9.

    Article  CAS  Google Scholar 

  22. Downs SM, Humpherson PG, Leese HJ. Meiotic induction in cumulus cell-enclosed mouse oocytes: involvement of the pentose phosphate pathway. Biol Reprod. 1998;58:1084–94.

    Article  PubMed  CAS  Google Scholar 

  23. Downs SM, Utecht AM. Metabolism of radiolabeled glucose by mouse oocytes and oocyte-cumulus cell complexes. Biol Reprod. 1999;60:1446–52.

    Article  PubMed  CAS  Google Scholar 

  24. Krisher RL, Bavister BD. Enhanced glycolysis after maturation of bovine oocytes in vitro is associated with increased developmental competence. Mol Reprod Dev. 1999;53:19–26.

    Article  PubMed  CAS  Google Scholar 

  25. Hashimoto S, Minami N, Yamada M, Imai H. Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents. Mol Reprod Dev. 2000;56:520–6.

    Article  PubMed  CAS  Google Scholar 

  26. Barnett DK, Clayton MK, Kimura J, Bavister BD. Glucose and phosphate toxicity in hamster preimplantation embryos involves disruption of cellular organization, including distribution of active mitochondria. Mol Reprod Dev. 1997;48:227–37.

    Article  PubMed  CAS  Google Scholar 

  27. Seshagiri PB, Bavister BD. Glucose and phosphate inhibit respiration and oxidalive metabolism in cultured hamster eight-cell embryos: evidence for the ‘Crabtree effect’. Mol Reprod Dev. 1991;30:105–11.

    Article  PubMed  CAS  Google Scholar 

  28. Seshagiri PB, Bavister BD. Glucose inhibits development of hamster 8-cell embryos in vitro. Biol Reprod. 1989;40:599–606.

    Article  PubMed  CAS  Google Scholar 

  29. Chung YG, Sarkhel BE, Seidel GE. Effect of fructose, follicle size, and cumulus oocyte quality on bovine embryo development in vitro. Biol Reprod. 1996;54:90.

    Google Scholar 

  30. Menezo Y, Khatchadourian C. Implication of glucose 6 phosphate isomerase (EC 5.3.1.9) activity in blocking sermentation of the mouse ovum at the 2 cell stage in vitro. C R Acad Sci III. 1990;310:297–301.

    PubMed  CAS  Google Scholar 

  31. Bhuiyan MMU, Kang SK, Lee BC. Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos. Zygote. 2007;15:1–10.

    Article  Google Scholar 

  32. Rieger D, Loskutoff NM, Betteridge KJ. Developmentally related changes in the uptake and metabolism of glucose, glutamine and pyruvate by cattle embryos produced in vitro. Reprod Fertil Dev. 1992;4:547–57.

    Article  PubMed  CAS  Google Scholar 

  33. Tsujii H, Taniguchi N, Hamano K. Development of Mongolian gerbil embryos in chemically defined media: effects of osmolarity, glucose and phosphate. J Reprod Dev. 2004;50:653–9.

    Article  PubMed  CAS  Google Scholar 

  34. Tsujii H, Nakamura Y. Utilization of [U–14C]glucose by preimplantation rat embryo cultured in vitro. J Mamm Ova Res. 1999;16:94–7.

    Article  Google Scholar 

  35. Guixe V, Prelier A, Kessi E, Ureta T. Regulatory role of fructose-2, 6-bisP on glucose metabolism in frog oocytes: in vivo inhibition of glycogen synthesis. Arch Biochem Biophys. 1997;348:75–81.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Animal Biotechnology, Shinshu University, Minamiminowa-mura, Nagano, 399-4598, Japan

    H. Tsujii, J. H. Lee, M. S. Hossain, K. M. A. Tareq & K. Hamano

  2. Nagoya Reproduction Center, The Sawada Women’s Clinic, Chikusaku, Nagoya, Aichi, Japan

    T. Sawada

Authors
  1. H. Tsujii
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  2. J. H. Lee
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  3. M. S. Hossain
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  4. K. M. A. Tareq
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  5. K. Hamano
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  6. T. Sawada
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Corresponding author

Correspondence to H. Tsujii.

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Tsujii, H., Lee, J.H., Hossain, M.S. et al. The beneficial effect of fructose and glucose on in vitro maturation and the fertilization of porcine oocytes. Reprod Med Biol 8, 19–24 (2009). https://doi.org/10.1007/s12522-008-0003-8

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  • DOI: https://doi.org/10.1007/s12522-008-0003-8

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