Abstract
Dietary habits are inferred through dental microwear analysis in humans from two Chalcolithic sites located on the Iberian Northern Plateau: El Portalón de Cueva Mayor and El Alto de la Huesera. The pattern of dental microwear was established on the buccal surfaces of permanent and deciduous molars, on the bottom of facet 9 on the occlusal surface of lower molars and on the incisal surfaces of deciduous incisors. Our findings suggest that during the Chalcolithic, the diet of populations from the Northern Plateau is less abrasive than that at the Mediterranean coast, due mainly to high meat consumption. The differences in diet are related to environmental factors, which are more appropriate for animal husbandry on the Northern Plateau. The consumption of meat is not equivalent in sub-adult and adult individuals from our samples located on the Northern Plateau. Younger individuals show a harder diet with less meat intake than older ones.
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References
Alday A, Pérez-Romero A, Carretero JM, Galindo-Pellicena MA, Adán G, Arsuaga JL (2015) Proofs of long distance relations between Central Europe and inland Iberian Peninsula during Neolithic and Bronze Age. Evidences from the material culture of the site of El Portalón (Sierra de Atapuerca, Burgos, Spain). Adv Anthropol 5:249–309
Alonso-Eguíluz M (2012) Estudio de los fitolitos en conjuntos de la Prehistoria reciente en la Sierra de Cantabria. El caso de los niveles de redil de San Cristóbal (Laguardia, Álava) CKQ 2:1–14
AlQahtani SJ, Hector MP, Liversidge HM (2010) Brief communication: the London atlas of human tooth development and eruption. Am J Phys Anthropol 142:481–490
Angelucci DE, Boschian G, Fontanals M, Pedroti A, Vergés JM (2009) Shepherds and karst: the use of caves and rock-shelters in the Mediterranean region during the Neolithic. World Archaeol 41:191–214
Brothwell DR (1981) Digging up bones: the excavation, treatment, and study of human skeletal remains. Cornell University Press, Nueva York
Bullington J (1991) Deciduous dental microwear of prehistoric juveniles from the lower Illinois River valley. Am J Phys Anthropol 84:59–73
Carretero JM, Ortega AI, Juez L, Pérez-González A, Arsuaga JL, Pérez R, Ortega MC (2008) A late pleistocene-early Holocene archaeological sequence of Portalón de Cueva mayor (Sierra de Atapuerca, Burgos, Spain). Munibe 59:67–80
Castilla M, Carretero JM, Gracia A, Arsuaga JL (2014) Evidence of rickets and/or scurvy in a complete Chalcolithic child skeleton from the El Portalón site (Sierra de Atapuerca, Spain). J Anthropol Sci 92:257–271
Clutton-Brock TH, Harvey PH (1977) Primate ecology and social organization. J Zool 183:1–39
El Zaatari S (2010) Occlusal microwear texture analysis and the diets of historical/prehistoric hunter-gatherers. Int J Osteoarchaeol 20:67–87
El Zaatari S, Hublin JJ (2014) Diet of Upper Paleolithic modern humans: evidence from microwear texture analysis. Am J Phys Anthropol 153:570–581
Fernández-Crespo T, de-la-Rúa C (2016) Demographic differences between funerary caves and megalithic graves of northern Spanish Late Neolithic/Early Chalcolithic. Am J Phys Anthropol 160:284–297
Fernández-Crespo T, Schulting RJ (2017) Living different lives: early social differentiation identified through linking mortuary and isotopic variability in Late Neolithic/Early Chalcolithic north-central Spain. PLoS One 12:1–19
Fernández-Eraso JF (2010) La actividad pecuaria en la Rioja Alavesa durante la Prehistoria Reciente. Cuad de Arqueol de la Univ de Navar 18:159–171
Fernández-Eraso J, Mujika-Alustiza JA (2013) La estación megalítica de La Rioja alavesa: cronología, orígenes y ciclos de utilización/The megalithic station of the Rioja Alavesa: chronology, origins and utilisation cycles. Zephyrus 71:89
Galbany J, Martínez LM, López-Amor HM, Espurz V, Hiraldo O, Romero A, De Juan J, Pérez-Pérez A (2005) Error rates in buccal-dental microwear quantification using scanning electron micorscopy. Scanning 27:23–29
Galbany J, Garriga N, Majoral Salich N, Coll Monteagudo R, Fluxá J (2008) Microdesgaste y patología dental en la población de la Edad de Bronce de “Mar i Muntanya” (Alella, Barcelona). Rev Esp Antrop Fís 28:25–36
Galindo-Pellicena MA (2014) Estudio de la macrofauna de los niveles holocenos del yacimiento de El Portalón (sierra de Atapuerca, Burgos) Dissertation. University Complutense of Madrid (unpublished thesis)
Galindo-Pellicena M, Carretero J., Arsuaga JL (2013) La gestión de Ovis aries y Capra hircus en los niveles calcolítico y bronce del yacimiento de El Portalón (Atapuerca, Burgos). Actas de las III Jorn de jóvenes investigadores del valle del Duero 3:83–100
Galindo-Pellicena MA, Carretero JM, Arsuaga JL (2014) Primary or secondary products? The nature of Ovis and Capra exploitation within the Bronze Age levels at Portalón (Atapuerca hill, Burgos, Spain). In: Greenfield HJ (ed) The archaeology of animal secondary products exploitation in Eurasia: from Europe to China. Oxbow Press, Oxford, pp 103–128
Galindo-Pellicena MA, Martín-Francés L, Gracia A, De Gaspar I, Arsuaga JL, Carretero JM (2017) Evidences of the use of cattle as draught animals in Chalcolithic of El Portalón (Sierra de Atapuerca, Burgos). Quat Int 438:1–10
Gamza T, Irish J (2012) A comparison of archaeological and dental evidence to determine diet at a predynastic Egyptian site. Int J Osteoarchaeol 22:398–408
García-González R, Carretero JM, Richards MP, Rodríguez L, Quam R (2015) Dietary inferences through dental microwear and isotope analyses of the Lower Magdalenian individual from El Mirón Cave (Cantabria, Spain). J Archaeol Sci 60:28–38
Gordon KD (1982) A study of microwear on chimpanzee molars: implications for dental microwear analysis. Am J Phys Anthropol 59:195–215
Grine FE (2005) Enamel thickness of deciduous and permanent molars in modern Homo sapiens. Am J Phys Anthropol 126:14–31
Grine FE (2007) Dentition and diet: introduction. In: Bailey SE, Hublin JJ (eds) Dental perspectives on human evolution. Springer, Dordrecht, pp 291–302
Grine FE, Ungar PS, Teaford MF (2002) Error rates in dental microwear quantification using scanning electron microscopy. Scanning 24:144–153
Hillson S (1996) Dental anthropology. Cambridge University Press, Cambridge
Hogue SH, Melsheimer R (2008) Integrating dental microwear and isotopic analyses to understand dietary change in east-central Mississippi. J Archaeol Sci 35:228–238
Krueger KL, Ungar PS (2009) Incisor microwear textures of five bioarcheological groups. Int J Osteoarchaeol 20:549–560
Lalueza C, Péréz-Perez A, Turbón D (1996) Dietary inferences through buccal microwear analysis of Middle and Upper Pleistocene human fossils. Am J Phys Anthropol 100:367–387
Larsen CS (1997) Bioarchaeology: interpreting behavior from the human skeleton. Cambridge University Press, Cambridge
Liversidge HM, Molleson T (2004) Variation in crown and root formation and eruption of human deciduous teeth. Am J Phys Anthropol 123:172–180
Lovejoy CO (1985) Dental wear in the Libben population: Its functional pattern and role in the determination of adult skeletal age at death. Am J Phys Anthropol 68: 47-56
Luschei ES, Goodwin GM (1974) Patterns of mandibular movement and jaw muscle activity during mastication in the monkey. J Neurophysiol 37:954–966
Mahoney P (2006a) Dental microwear from Natufian hunter-gatherers and early Neolithic farmers: comparisons within and between samples. Am J Phys Anthropol 130:308–319
Mahoney P (2006b) Brief communication: intertooth and intrafacet dental microwear variation in an archaeological sample of modern humans from the Jordan Valley. Am J Phys Anthropol 129:39–44
Mahoney P (2006c) Microwear and morphology: functional relationships between human dental microwear and the mandible. J Hum Evol 50:452–459
Mahoney P (2007) Human dental microwear from Ohalo II (22,500–23,500 cal BP), Southern Levant. Am J Phys Anthropol 132:489–500
Mahoney P, Schmidt CW, Deter C, Remy A, Slavin P, Johns SE, Miszkiewicz JJ, Nystrom P (2016) Deciduous enamel 3D microwear texture analysis as an indicator of childhood diet in medieval Canterbury, England. J Archaeol Sci 66:128–136
Martínez-Pillado V, Aranburu A, Arsuaga JL, Ruiz-Zapata B, Gil-Garcías MJ, Stoll H, Yusta I, Iriarte I, Carretero JM, Edwards L, Cheng H (2014) Upper Pleistocene and Holocene palaeoenvironmental records in Cueva Mayor karst (Atapuerca, Spain) from different proxies: speleothem crystal fabrics, palynology, and archaeology. Int J Speleol 43:1–14
McClure S, García-Puchol O, Roca C, Culleton B, Kennett D (2011) Osteological and paleodietary investigation of burials from Cova la Pastora, Alicante, Spain. J Archaeol Sci 38:420–428
Milner GR, Larsen CS (1991) Teeth as artifacts of human behavior: intentional mutilation and accidental modification. In: Ma K, Larsen CS (eds) Advances in dental anthropol. Wiley, New York, pp 357–378
Molleson T, Jones K (1991) Dental evidence for dietary change at Abu Hureyra. J Archaeol Sci 18:525–539
Molleson T, Jones K, Jones S (1993) Dietary change and the effects of food preparation on microwear patterns in the Late Neolithic of Abu Hureyra, northern Syria. J Hum Evol 24:455–468
Moorrees CF, Fanning EA, Hunt EE Jr (1963) Age variation of formation stages for ten permanent teeth. J Dental Res 42:1490–1502
Pérez Díaz S, Ruiz Alonso M, López Sáez JA, Zapata Peña L (2010) Dinámica vegetal y antropización en la Sierra de Cantabria (Álava) desde el Neolítico a la Edad del Bronce. Polen 20:25–40
Pérez-Pérez A, Lalueza C, Turbón D (1994) Intraindividual and intragroup variability of buccal tooth striation pattern. Am J Phys Anthropol 94:175–187
Pérez-Pérez A, Bermúdez de Castro JM, Arsuaga JL (1999) Nonocclusal dental microwear analysis of 300,000-year-old Homo heilderbergensis teeth from Sima de los Huesos (Sierra de Atapuerca, Spain). Am J Phys Anthropol 108:433–457
Pérez-Pérez A, Espurz V, Bermúdez de Castro JM, de Lumley MA, Turbón D (2003) Non-occlusal dental microwear variability in a sample of Middle and Late Pleistocene human populations from Europe and the Near East. J Hum Evol 44:497–513
Pérez-Pérez A, Lozano M, Romero A, Martínez LM, Galbany J, Pinilla B, Estebaranz-Sánchez F, Bermúdez de Castro JM, Carbonell E, Arsuaga JL (2018) The diet of the first Europeans from Atapuerca. Sci Rep 7:43318. https://doi.org/10.1038/srep43319
Pérez-Romero A, Iriarte E, Galindo-Pellicena MA, García-González R, Rodríguez L, Castilla M, Francés-Negro M, Santos E, Valdiosera C, Arsuaga JL, Alday A, Carretero JM (2017) An unusual Pre-bell beaker copper age cave burial context from El Portal_on de Cueva Mayor site (Sierra de Atapuerca, Burgos). Quat Int 433:142–155
Polo-Cerdá M, Romero A, Casabó J, de Juan J (2007) The Bronze Age burials from Cova dels Blaus (Vall d'Uixó, Castelló, Spain): an approach to paleodietary reconstruction through dental pathology, occlusal wear and buccal microwear patterns. J Comparative Hum Biol 58:297–307
Richards LC, Miller SLJ (1991) Relationships between age and dental attrition in Australian Aboriginals. Am J Phys Anthropol 84:159–164
Romero A, De Juan J (2007) Intra-and inter-population human buccal tooth surface microwear analysis: inferences about diet and formation processes. Anthropologie 45:61–70
Romero A, Martínez-Ruíz N, De Juan J (2004) Non-occlusal dental microwear in a Bronze-Age human sample from East Spain. Anthropologie 42:65–70
Romero A, Galbany J, De Juan J, Pérez-Pérez A (2012) Brief communication: short- and long -term in vivo human dental buccal-dental microwear. Am J Phys Anthropol 148:467–472
Romero A, Ramírez-Rozzi FV, De Juan J, Pérez-Pérez A (2013) Diet-related buccal dental microwear patterns in Central African pygmy foragers and bantu-speaking farmer and pastoralist populations. PLoS One 8(12):e84804
Salazar-García DC (2009) Estudio de la dieta en la población neolítica de Costamar. Resultados preliminares de análisis de isótopos estables de carbono y nitrógeno. In: Flors Ureña E (ed) Evolución del paisaje antrópico desde la prehistoria hasta el medioevo. Servicio de Investigaciones Arqueológicas y Prehistóricas. Diputación de Castellón, Castellón, pp 411–418
Salazar-García DC (2011) Patrón de dieta en la población púnica de Can Marines (Ibiza) a través del análisis de isótopos estables (C y N) en colágeno óseo. Saguntum 43:95–102
Salazar-García DC (2014) Estudio de la dieta en la población de Cova dels Diablets mitjançant anàlisi d'isòtops estables del carboni i del nitrogen en col·làgen ossi. Resultats preliminars. In: Aguilella Arzo G, Roman in Monroig D, García Borja P (eds) Prehistòria a la Serra d'Irta. Diputació de Castelló, Castellón, pp 67–78
Salazar-García DC, Vives-Ferrándiz J, Fuller B, Richards MP (2010) Alimentación estimada de la población del Castellet de Bernabé (ss. V-III a.C.) mediante el uso de ratios de isótopos estables de C y N. Saguntum 9:313–322
Salazar-García DC, Romero A, García-Borja P, Subirá ME, Richards MP (2016) A combined dietary approach using isotope and dental buccal-microwear analysis of human remains from the Neolithic, Roman and Medieval periods from the archaeological site of Tossal de les Basses (Alicante, Spain). J Archaeol Sci Rep
Schmidt CW (2001) Dental microwear evidence for a dietary shift between two nonmaize-reliant prehistoric human populations from Indiana. Am J Phys Anthropol 114:139–145
Scott RM, Halcrow SE (2017) Investigating weaning using dental microwear analysis: a review. J Archaeol Sci Rep 11:1–11
Scott RS, Ungar PS, Bergstrom TS, Brown CA, Grine FE, Teaford MF, Walker A (2005) Dental microwear texture analysis shows within-species diet variability in fossil hominins. Nature 436:693–695
Scott RS, Ungar PS, Bergstrom TS, Brown CA, Childs BE, Teaford MF, Walker A (2006) Dental microwear texture analysis: technical considerations. J Hum Evol 51:339–349
Scott RS, Teaford MF, Unga PS (2012) Dental microwear texture and antrhopoid diets. Am J Phys Anthropol 147:551–579
Smith BH (1991) Standards of human tooth formation and dental age assessment. Wiley-Liss Inc., New Jersey
Teruyuki H (2005) Regional differences of dental microwear on the occlusal surface of an M2 from Neolithic Japan: a case study. Dent Anthropol 18:61–64
Ungar PS, Spencer MA (1999) Incisor microwear, diet, and tooth use in three Amerindian populations. Am J Phys Anthropol 109:387–396
White TD, Black MT, Folkens PA (2011) Human osteology. Academic Press, Cambridge
Acknowledgements
We thank our colleagues from the Laboratorio de Evolución Humana and the El Portalón research team for their help during field seasons and for their useful comments and critical review of the manuscript. We also thank Prof. J. Fernández-Eraso and Drs. J. A. Mujika and Fernández-Crespo for the facilities to collect the samples of Alto de la Huesera. Special thanks to three anonymous reviewers which notably improve this work.
Funding
The authors received financial support from the Ministerio de Economía y Competitividad, Spain (project CGL2015-65387-C3-2-P, MINECO-FEDER). Excavations in El Portalón are funded by the Junta de Castilla y León and Fundación Atapuerca.
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García-González, R., Sánchez-Puente, Z., Arsuaga, J.L. et al. Dietary inferences from dental microwear patterns in Chalcolithic populations from the Iberian Peninsula: the case of El Portalón de Cueva Mayor (Sierra de Atapuerca, Burgos, Spain) and El Alto de la Huesera (Álava, Spain). Archaeol Anthropol Sci 11, 3811–3823 (2019). https://doi.org/10.1007/s12520-018-0711-x
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Keywords
- Chalcolithic diet
- Northern Plateau
- Age-related dietary habits