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Common WU polyomavirus infection in a Beijing population indicated by surveillance for serum IgG antibody against capsid protein VP2

World Journal of Pediatrics volume 9, pages 48–52 (2013)Cite this article

Abstract

Background

WU polyomavirus (WU virus) was identified as a novel polyomavirus in 2007 from specimens of pediatric patients with acute respiratory infection (ARI). A lack of permissive cell lines has limited investigations into WU virus pathogenesis and prevalence.

Methods

The encoding region of the capsid protein VP2 gene was amplified from a WU virus DNA-positive clinical specimen and expressed as a recombinant Histagged protein in Escherichia coli BL21 (DE3). The expressed VP2 was identified by expected molecular weight and immunoreactivity with anti-His monoclonal antibody in Western blotting assay. Serum samples collected from 455 individuals of all ages in Beijing without symptoms of ARI were tested for IgG antibodies against the affinity-purified recombinant VP2 protein by Western blotting to investigate the prevalence of natural WU virus infection. In addition, serum samples from four ARI pediatric patients, whose nasopharyngeal aspirates were positive for WU virus DNA and negative for all other respiratory-related viruses, were tested for IgM antibody against the recombinant VP2.

Results

Of the 455 serum samples, 238 reacted with the recombinant VP2, yielding an overall positive rate of 52.3% for IgG against VP2 of WU virus. The positive rate was the highest in serum samples from infants and children between 1 to 4 years of age. One of four ARI pediatric patients was positive for IgM against WU virus VP2, implicating WU virus as the causative disease agent.

Conclusions

The high prevalence of IgG against WU polyomavirus in Beijing-based study population indicates that WU virus infection is common in Beijing. WU virus may be responsible for some pediatric ARI cases, and primary infection of this virus may occur mostly in childhood.

References

  1. Gaynor AM, Nissen MD, Whiley DM, Mackay IM, Lambert SB, Wu G, et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog 2007;3:e64.

    PubMed  Article  Google Scholar 

  2. Gardner SD, Field AM, Coleman DV, Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1971;1:1253–1257.

    CAS  Google Scholar 

  3. Padgett BL, Walker DL, ZuRhein GM, Eckroade RJ, Dessel BH. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet 1971;1:1257–1260.

    PubMed  Article  CAS  Google Scholar 

  4. Allander T, Andreasson K, Gupta S, Bjerkner A, Bogdanovic G, Persson MA, et al. Identification of a third human polyomavirus. J Virol 2007;81:4130–4160.

    PubMed  Article  CAS  Google Scholar 

  5. Feng H, Shuda M, Chang Y, Moore PS. Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 2008;319:1096–1100.

    PubMed  Article  CAS  Google Scholar 

  6. Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB. Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Cell Host Microbe 2010;7:509–515.

    PubMed  Article  CAS  Google Scholar 

  7. van der Meijden E, Janssens RW, Lauber C, Bouwes Bavinck JN, Gorbalenya AE, Feltkamp MC. Discovery of a new human polyomavirus associated with trichodysplasia spinulosa in an immunocompromized patient. PLoS Pathog 2010;6:e1001024.

    PubMed  Article  Google Scholar 

  8. Ringshausen FC, Heckmann M, Weissbrich B, Neske F, Borg I, Knoop U, et al. No evidence for WU polyomavirus infection in chronic obstructive pulmonarydisease. Infect Agent Cancer 2009;4:12–16.

    PubMed  Article  Google Scholar 

  9. van de Pol AC, Wolfs TF, Jansen NJ, Kimpen JL, van Loon AM, Rossen JW. Human bocavirus and KI/WU polyomaviruses in pediatric intensive care patients. Emerg Infect Dis 2009;15:454–457.

    PubMed  Article  Google Scholar 

  10. Wattier RL, Vázquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, et al. Role of human polyomaviruses in respiratory tract disease in young children. Emerg Infect Dis 2008;14:1766–1768.

    PubMed  Article  CAS  Google Scholar 

  11. Barzon L, Squarzon L, Militello V, Trevisan M, Porzionato A, Macchi V, et al. WU and KI polyomaviruses in the brains of HIV-positive patients with and without progressive multifocal leukoencephalopathy. J Infect Dis 2009;200:1755–1758.

    PubMed  Article  CAS  Google Scholar 

  12. Limaye AP, Jerome KR, Kuhr CS, Ferrenberg J, Huang ML, Davis CL, et al. Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients. J Infect Dis 2001;183:1669–1672.

    PubMed  Article  CAS  Google Scholar 

  13. Ren L, Gonzalez R, Xu X, Li J, Zhang J, Vernet G, et al. WU polyomavirus in fecal specimens of children with acute gastroenteritis, China. Emerg Infect Dis 2009;15:134–135.

    PubMed  Article  Google Scholar 

  14. Zhao L, Qian Y, Zhu R, Deng J, Wang F, Sun Y, et al. Identification of WU polyomavirus from pediatric patients with acute respiratory infections in Beijing, China. Arch Virol 2010;155:181–186.

    PubMed  Article  CAS  Google Scholar 

  15. Zhao L, Qian Y, Zhu R, Deng J, Wang F. Study on the status of human rhinovirus infections in infants and young children with acute respiratory infections in Beijing, from 2001 to 2006. Zhonghua Liu Xing Bing Xue Za Zhi 2007;28:683–685. [In Chinese]

    PubMed  CAS  Google Scholar 

  16. Zhu R, Qian Y, Zhao L, Deng J, Wang F. Surveillance on human metapneumovirus in infants and children with acute respiratory infections in Beijing, from 2004 to 2006. Zhonghua Liu Xing Bing Xue Za Zhi 2007;28:679–682. [In Chinese]

    PubMed  Google Scholar 

  17. Zhao L, Qian Y, Wang F, Zhu RN, Deng J. Human parainfluenza virus infections in infants and young children with acute respiratory infections in Beijing. Zhonghua Er Ke Za Zhi 2007;45:91–95. [In Chinese]

    PubMed  Google Scholar 

  18. Zhao L, Qian Y, Zhu R, Deng J, Wang F, Li Y. Preliminary studies suggest that a novel parvovirus called human bocavirus is related to acute respiratory infections in pediatric patients in Beijing. Zhonghua Wei Sheng Wu Xue Yu Mian Yi Xue Za Zhi 2006;26:385–388. [In Chinese]

    CAS  Google Scholar 

  19. Zhu R, Qian Y, Zhao L, Deng J, Wang F, Liao B. Human coronavirus-NL63 was detected in specimens from children with acute respiratory infections in Beijng, China. Zhonghua Er Ke Za Zhi 2006;44:202–205. [In Chinese]

    PubMed  Google Scholar 

  20. Kantola K, Sadeghi M, Ewald MJ, Weissbrich B, Allander T, Lindau C, et al. Expression and serological characterization of polyomavirus WUPyV and KIPyV structural proteins. Viral Immunol 2010;23:385–393.

    PubMed  Article  CAS  Google Scholar 

  21. Kean JM, Rao S, Wang M, Garcea RL. Seroepidemiology of human polyomaviruses. PLoS Pathog 2009;5:e1000363.

    PubMed  Article  Google Scholar 

  22. Nguyen NL, Le BM, Wang D. Serologic evidence of frequent human infection with WU and KI polyomaviruses. Emerg Infect Dis 2009;15:1199–1205.

    PubMed  Article  Google Scholar 

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Affiliations

  1. Laboratory of Virology, Capital Institute of Pediatrics, 2 Yabao Road, Beijing, 100020, China

    Ni-Na Zhang, Lin-Qing Zhao, Yuan Qian, Ru-Nan Zhu, Jie Deng, Fang Wang, Yu Sun & Li-Ying Liu

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  1. Ni-Na Zhang
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  2. Lin-Qing Zhao
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  3. Yuan Qian
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  4. Ru-Nan Zhu
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  5. Jie Deng
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  6. Fang Wang
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  7. Yu Sun
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  8. Li-Ying Liu
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Correspondence to Yuan Qian.

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Zhang, NN., Zhao, LQ., Qian, Y. et al. Common WU polyomavirus infection in a Beijing population indicated by surveillance for serum IgG antibody against capsid protein VP2. World J Pediatr 9, 48–52 (2013). https://doi.org/10.1007/s12519-013-0397-1

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  • DOI: https://doi.org/10.1007/s12519-013-0397-1

Key words

  • antibody
  • infection
  • virus
  • WU polyomavirus