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+276 G/T single nucleotide polymorphism of the adiponectin gene is associated with the susceptibility to biliary atresia

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Abstract

Background

Biliary atresia (BA) is an intractable neonatal inflammatory and obliterative cholangiopathy, leading to progressive fibrosis and cirrhosis. Adiponectin, an anti-inflammatory adipokine, is known to play a possible role in liver diseases. The objective of our study was to determine the relationship between adiponectin gene polymorphisms and BA susceptibility.

Methods

A total of 106 BA patients and 107 healthy controls were included in this study. Two single nucleotide polymorphisms (SNPs) of the adiponectin gene, +45T/G (rs2241766) and +276G/T (rs1501299), were genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis.

Results

Genotype distributions of +45 T/G and +276 G/T SNPs were seen in the Hardy-Weinberg equilibrium for both BA patients and controls. The frequency of the G/G genotype at +276G/T was significantly higher in BA patients than in the controls (P=0.009). Regarding +45T/ G in BA patients, the frequency of the T/T genotype tended to be lower than in the controls, but the difference was not significant. Moreover, the G allele at +276G/T in BA patients was more common than in the controls (P=0.0043). In contrast, the frequency of the T allele at +45T/G was not significantly different between BA patients and the controls. None of the haplotypes studied was found to significantly influence the risk of BA.

Conclusions

+276G/T SNP is strongly associated with BA, particularly with the G allele. We postulate that the +276G/T adiponectin gene polymorphism confers increased susceptibility to BA.

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Authors and Affiliations

  1. Program in Medical Sciences, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Wanvisa Udomsinprasert, Sittisak Honsawek & Wilai Anomasiri

  2. Center for Excellence in Omics-Nano Medical Technology Development Project, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Tewin Tencomnao

  3. Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Paisarn Vejchapipat

  4. Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand

    Voranush Chongsrisawat & Yong Poovorawan

  5. Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Rama IV road, Patumwan, Bangkok, 10330, Thailand

    Yong Poovorawan

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  1. Wanvisa Udomsinprasert
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  2. Tewin Tencomnao
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Correspondence to Yong Poovorawan.

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Udomsinprasert, W., Tencomnao, T., Honsawek, S. et al. +276 G/T single nucleotide polymorphism of the adiponectin gene is associated with the susceptibility to biliary atresia. World J Pediatr 8, 328–334 (2012). https://doi.org/10.1007/s12519-012-0377-x

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  • DOI: https://doi.org/10.1007/s12519-012-0377-x

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