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Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites

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Abstract

Background

The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions.

Methods

Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation.

Results

After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production.

Conclusion

SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens.

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Authors and Affiliations

  1. Division of Pediatrics, Tor Vergata University, Rome, Italy

    Federica Angelini, Stefania Corrente, Romina Silenzi, Antonella Polito, Silvia Di Cesare, Marie Laure Yammine, Paolo Rossi, Viviana Moschese & Loredana Chini

  2. DPUO, Bambino Gesù Children’s Hospital, Rome, Italy

    Federica Angelini, Valentina Pacciani & Paolo Rossi

  3. Division of Neonatology, Bambino Gesù Children’s Hospital, Rome, Italy

    Alessandra Di Pede

  4. Division of Pediatric Medicine, Bambino Gesù Children’s Hospital, Rome, Italy

    Carla Riccardi

  5. University Department of Pediatrics (DPUO), Bambino Gesù Children’s Hospital, Piazza S.Onofrio 4, 00165, Roma, Italy

    Federica Angelini

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  1. Federica Angelini
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  2. Valentina Pacciani
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Correspondence to Federica Angelini.

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Angelini, F., Pacciani, V., Corrente, S. et al. Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites. World J Pediatr 7, 24–30 (2011). https://doi.org/10.1007/s12519-011-0242-3

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  • DOI: https://doi.org/10.1007/s12519-011-0242-3

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