Skip to main content
SpringerLink
Log in

Molecular epidemic features and variation of rotavirus among children with diarrhea in Lanzhou, China, 2001–2006

  • Original Article
  • Published:
World Journal of Pediatrics Aims and scope Submit manuscript

Abstract

Background

Human rotavirus (HRV) is the most common pathogen causing severe diarrhea among infants and young children worldwide. This study aims to understand rotavirus epidemiology and its variation in the period of 2001–2006 in Lanzhou, Gansu Province, China, and to provide an epidemiological basis for the development of rotavirus vaccine.

Methods

A total of 1019 stool specimens were collected from patients with acute diarrhea admitted to the First Hospital of Lanzhou University from 2001 to 2006, who were younger than 5 years old. Dako IDEIATM kits were used for detection of rotavirus, and RT-PCR was performed for determination of G serotype and P genotype of the rotavirus.

Results

Rotavirus was present in 51.6% (526) of the 1019 specimens. G serotype identified G3 at 40.9%, G2 14.6%, G1 22.2% and G9 1.9%. Mixed-G infection was observed in 4.4% and non-typeable infection 16.0%. P genotype was observed in 372 samples, of which P[8] accounted for 186 cases (50.0%), P[4] 72 cases (19.4%), mixed-P infection 2 cases (0.5%), and non-typeable cases 112 (30.1%). G3 was the most prevalent G serotype found in this study from 2001 to 2004, G2 was the most prevalent G serotype (34.4%) from 2004 to 2005, and G1 (61.5%) was the most prevalent strain from 2005 to 2006. G9 was detected in 10 cases (1.9%) and G4 was not detected during this 5-year period. P[8] was the most prevalent P genotype found over the 5 consecutive years of this study, although there was a significant transition of P genotype from 2004 to 2005 with P[4] (45%) identified as the predominant P genotype, followed by P[8] (22.1%). The predominant G-P combination was P[8]G1 (33.6%), followed by P[8]G3 (32.1%) and P[4]G2 (17.2%). Rotavirus diarrhea admissions peaked between October and December. Continuous surveillance showed that the incidence rate of rotavirus was the highest in infants aged 6–23 months, averaging 11.0–11.9 months.

Conclusions

Five years of continuous surveillance showed that rotavirus remains the most significant viral agent causing diarrhea hospitalization among children under 5 years old in Lanzhou, China although the predominant strain of rotavirus varies between years. Mixed-G serotype infection also appears to occur at a relatively high rate in Lanzhou.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI. Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 2003;9:565–572.

    PubMed  Google Scholar 

  2. Shinozaki K, Okada M, Nagashima S, Kaiho I, Taniguchi K. Characterization of human rotavirus strains with G12 and P[9] detected in Japan. J Med Virol 2004;73:612–616.

    Article  PubMed  CAS  Google Scholar 

  3. Martella V, Ciarlet M, Bányai K, Lorusso E, Cavalli A, Corrente M, et al. Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 2006;346:301–311.

    Article  PubMed  CAS  Google Scholar 

  4. Rivest P, Proulx M, Lonergan G, Lebel MH, Bédard L. Hospitalisations for gastroenteritis: the role of rotavirus. Vaccine 2004;22:2013–2017.

    Article  PubMed  Google Scholar 

  5. Iturriza-Gómara M, Green J, Brown DW, Ramsay M, Desselberger U, Gray JJ. Molecular epidemiology of human group A rotavirus infections in the United Kingdom between 1995 and 1998. J Clin Microbiol 2000;38:4394–4401.

    PubMed  Google Scholar 

  6. Fang ZY, Qi J, Yang H, Wang CX, Ye Q, Ma L, et al. Serotype and genotype study of childhood rotaviruses isolated in 1998–1999 in China. Chin J Virol 2001;17:17–23.

    Google Scholar 

  7. Fang ZY, Jin SJ, Qin SM, Zhao XF, Wu D, Shijima HU, et al. Serotypes of group A rotavirus isolates determined by PCR in Hebei and Henan provinces, China. Chin J Virol 1994;10:316–321.

    CAS  Google Scholar 

  8. Zhang LJ, Du ZQ, Zhang Q, Kang HY, Zheng LSH, Liu XM, et al. Rotavirus surverillance date from Kunming Children’s Hospital, 1998–2001. Chin J Epidemiol 2004;25:396–399.

    Google Scholar 

  9. Gil A, Carrasco P, Jiménez R, San-Martín M, Oyagüez I, González A. Burden of hospitalizations attributable to rotavirus infection in children in Spain, period 1999–2000. Vaccine 2004;22:2221–2225.

    Article  PubMed  CAS  Google Scholar 

  10. Fang ZY, Wang B, Kilgore PE, Bresee JS, Zhang LJ, Sun LW, et al. Sentinel hospital surveillance for rotavirus diarrhea in the People’s Republic of China, August 2001–July 2003. J Infect Dis 2005;192Suppl 1:S94–S99.

    Article  PubMed  Google Scholar 

  11. Ye XH, Jin Y, Fang ZY, Song YP, Xie HP, Zhang Q, et al. Etiological study on viral diarrhea among children in Lanzhou, China, 2004–2005. Chin J Epidemiol 2006;27:117–122.

    Google Scholar 

  12. Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, et al. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 1987;25:1757–1762.

    PubMed  CAS  Google Scholar 

  13. Qian Y, Yuan LJ, Xiong CH, Zhang Y, Liu J, Guan DH, et al. Identification of rotavirus G9 type from a stool specimen collected from a child with diarrhea in Beijing. Chin J Virol 1994;10:263–267.

    Google Scholar 

  14. Tian JM, Xu L, Sheng H, Jin H. Study on the clinical epidemiology of young children under 5 years old diarrhea caused by rotavirus in Suzhou area. Pediatr Emerg Med 2003;10:370–371.

    Google Scholar 

  15. Fang ZY, Yang H, Qi J, Zhang J, Sun LW, Tang JY, et al. Diversity of rotavirus strain among children with acute diarrhea in China: 1998–2000 surveillance study. J Clin Microbiol 2002;40:1875–1878.

    Article  PubMed  Google Scholar 

  16. Zhang Q, Duncan S, Roger G, Fang ZY, Wang DK, Ye XH, et al. Molecular epidemiological research on rotavirus serotype G9 isolated from 9 regions in China. Chin J Vaccines Immunization 2006;12:476–479.

    Google Scholar 

  17. Bahl R, Ray P, Subodh S, Shambharkar P, Saxena M, Parashar U, et al. Incidence of severe rotavirus diarrhea in New Delhi, India, and G and P types of the infecting rotavirus strains. J Infect Dis 2005;192Suppl 1:S114–119.

    Article  PubMed  Google Scholar 

  18. Jain V, Das BK, Bhan MK, Glass RI, Gentsch JR. Indian Strain Surveillance Collaborating Laboratories. Great diversity of group A rotavirus strains and high prevalence of mixed rotavirus infections in India. J Clin Microbiol 2001;39:3524–3529.

    Article  PubMed  CAS  Google Scholar 

  19. Unicomb LE, Podder G, Gentsch JR, Woods PA, Hasan KZ, Faruque AS, et al. Evidence of high-frequency genomic reassortment of group A rotavirus strains in Bangladesh: emergence of type G9 in 1995. J Clin Microbiol 1999;37:1885–1891.

    PubMed  CAS  Google Scholar 

  20. Moon SS, Green YS, Song JW, Ahn CN, Kim H, Park KS, et al. Genetic distribution of group A human rotavirus types isolated in Gyunggi province of Korea, 1999–2002. J Clin Virol 2007;38:57–63.

    Article  PubMed  CAS  Google Scholar 

  21. Kheyami AM, Nakagomi T, Nakagomi O, Dove W, Hart CA, Cunliffe NA. Molecular epidemiology of rotavirus diarrhea among children in Saudi Arabia: first detection of G9 and G12 strains. J Clin Microbiol 2008;46:1185–1191.

    Article  PubMed  CAS  Google Scholar 

  22. Le VP, Kim JY, Cho SL, Nam SW, Lim I, Lee HJ, et al. Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea. J Med Virol 2008;80:175–182.

    Article  PubMed  CAS  Google Scholar 

  23. Matthijnssens J, Rahman M, Yang X, Delbeke T, Arijs I, Kabue JP, et al. G8 rotavirus strains isolated in the Democratic Republic of Congo Belong to the DS-1-Like Genogroup. J Clin Microbiol 2006;44:1801–1809.

    Article  PubMed  CAS  Google Scholar 

  24. Duan ZJ, Li DD, Zhang Q, Liu N, Zheng LS, Tang JY, et al. Discovery of a human group A rotavirus strain with G5 specificity in Lulong County of China. Chin J Virol 2007;23:96–101.

    CAS  Google Scholar 

  25. Macedo CI, Christofoletti A, Munford V, Rácz ML. G and P rotavirus genotypes in stool samples from children in Teresina, State of Piauí. Rev Soc Bras Med Trop 2007;40:381–384.

    Article  PubMed  Google Scholar 

  26. Fang ZY, Zhang LJ, Tang JY, Zhang Q, Hu HK, Xie HP, et al. Rotavirus diarrhea among children in Lulong County, Hebei Province, China. Chin J Virol 2005;21:21–25.

    Google Scholar 

  27. Castello AA, Arvay ML, Glass RI, Gentsch J. Rotavirus strain surveillance in Latin America: a review of the last nine years. Pediatr Infect Dis J 2004;23:S168–S172.

    Article  PubMed  Google Scholar 

  28. Nguyen TV, Le Van P, Le Huy C, Weintraub A. Diarrhea caused by rotavirus in children less than 5 years of age in Hanoi, Vietnam. J Clin Microbiol 2004;42:5745–5750.

    Article  PubMed  Google Scholar 

  29. Suzuki H, Sakai T, Tanabe N, Okabe N. Peak rotavirus activity shifted from winter to early spring in Japan. Pediatr Infect Dis J 2005;24:257–260.

    Article  PubMed  Google Scholar 

  30. Zhang CF, Jin Y, Zhang Y, Qian Y. VP4 and VP7 typing for rotaviruses identified from stool specimens collected from infants and young children with acute diarrhea in Lanzhou. Chin J Pediatr 2002;40:409–412.

    Google Scholar 

  31. Dong QL, Jin Y, Zang Q, Xie HP, Fang ZY. Clinical study on the characteristic patterns of the diarrhea caused by rotavirus and calicivirus in infants and young children in Lanzhou. J Clin Pediatr 2005;23:364–367.

    Google Scholar 

  32. Jin Y, Huang X, Fang ZY, Tan JY, Xie HP, Wang DK, et al. Molecular epidemiology of virus diarrhea among infants and young children in Lanzhou. Chin J Pract Pediatr 2006;21:15–18.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lanzhou, China

    Yu Jin, Xin-Hua Ye, Zhao-Yin Fang, Yu-Ning Li, Xue-Mei Yang, Qiao-Li Dong & Xiang Huang

  2. The First Hospital of Lanzhou University, Lanzhou, 730000, China

    Yu Jin, Xin-Hua Ye, Yu-Ning Li, Xue-Mei Yang, Qiao-Li Dong & Xiang Huang

  3. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China

    Zhao-Yin Fang

Authors
  1. Yu Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin-Hua Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhao-Yin Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu-Ning Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xue-Mei Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qiao-Li Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xiang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhao-Yin Fang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, Y., Ye, XH., Fang, ZY. et al. Molecular epidemic features and variation of rotavirus among children with diarrhea in Lanzhou, China, 2001–2006. World J Pediatr 4, 197–201 (2008). https://doi.org/10.1007/s12519-008-0036-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12519-008-0036-4

Key words

Search

Navigation