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The effectiveness of rhizosphere microorganisms to control Verticillium wilt disease caused by Verticillium dahliae Kleb. in olives

  • ICCESEN 2017
  • Published:
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Abstract

Soil is an important natural resource for the existence of plants, animals, and microorganisms. In this study, the effectiveness of some biological preparations from soil-derived microorganisms against Verticillium wilt caused by Verticillium dahliae, which is a very important disease in olive, was investigated. Harpin protein (Messenger Gold), Mycorrhiza (ERS)-Trichoderma harzianum strain (T22 Planter Box), and different combinations of these treatments were used as biological preparations against V. dahliae. Additionally, the effectiveness of Bordeaux mixture, which is an important component in sustainable agriculture and is widely used by the farmers, was added to this study in order to compare soil microorganisms. Plants have been observed every month for a year, and after 12 months, the plants were evaluated according to the scale of 0–4. As a result, high efficacy results have been obtained in Messenger Gold, Messenger Gold + ERS-T22 Planter Box + Bordeaux mixture, and Messenger Gold + Bordeaux mixture applications (P ≤ 0.05). Application of Messenger Gold and in combination with Messenger Gold suppressed the Verticillium wilt disease in the parts of the trees where the symptoms occurred, and these trees showed regeneration by new shoot development. The application of ERS-T22 Planter Box and Bordeaux mixture suppressed the Verticillium wilt, and the disease symptoms have not spread to the other parts of plants. However, no shoot formation occurred in these diseased parts. According to our results, it is considered that Harpin protein may have a positive effect on the activation of xylem in olive and induces the pathogen host defenses. In addition, the presence of some beneficial microorganisms can restrict the fungal sporulation and growth of V. dahliae. Therefore the Harpin protein can be used as a biological control agent in combination with other antagonism microorganisms.

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References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    Article  Google Scholar 

  • Akhtar MS, Siddiqui MA (2008) Arbuscular mycorrhizal fungi as potential bioprotectants against plant pathogens. In: Siddiqui ZA, Akhtar S, Futai K (eds) Mycorrhizae: sustainable agriculture and forestry. Springer, Dordrecht, pp 61–98

    Chapter  Google Scholar 

  • Alabouvette C, Olivain C, Migheli Q, Steinberg C (2009) Microbiological control of soil-borne phytopathogenic fungi with special emphasis on wilt-inducing Fusarium oxysporum. New Phytol 184:529–544

    Article  Google Scholar 

  • Anonnymous (2013) Zeytin Yetiştiriciliği. Gıda Tarım ve Hayvancılık Bakanlığı, Aydın İl Müdürlüğü, Çiftçi Eğitim Serisi Yayın No:2013-02, 5-6 s. Aydın

  • Arora NK (eEd.) (2015) Plant microbes symbiosis: applied facets. Springer, Dordrecht, pp 381

  • Arora NK, Verma M, Prakash J, Mishra J (2016) Regulation of biopesticides: global concerns and policies. In: Arora et al (eds) Bioformulations: For Sustainable Agriculture. Springer, India, pp 283–299

    Google Scholar 

  • Bartoli CG, Casalongué CA, Simontacchi M, Marquez-Garcia B, Foyer CH (2013) Interactions between hormone and redox signalling pathways in the control of growth and cross tolerance to stress. Environ Exp Bot 94:73–88

    Article  Google Scholar 

  • Benitez T, Rincon AM, Limon MC, Codon AC (2004) Biocontrol mechanism of Trichoderma strains. Int Microbiol 7:249–260

    Google Scholar 

  • Benlioğlu S, Demirbaş M, Ulusal H (2000) Aydın ilinde zeytin ağaçlarında görülen kurumalarla ilgili yayınlanmamış survey raporu. AD. Ü. Ziraat Fakültesi, Bitki Koruma Bölümü

  • Blanco-Lopez MA, Jimenez-Diaz RM, Caballero JM (1984) Symptomatology, incidence and distribution of Verticillium wilt of olive trees in Andalucia. Phytopath. Medit., 23: 1-8 (in R. P. P., 64: 1674, 1975)

  • Bourbos VA, Barbopoulou EA (2006) Effect of Harpin Ea on the fruit productıon and control of Phytophthora infestans in greenhouse tomato. Acta Hortic 727: 566–570

  • Çaltılı O, Arıcı ŞE (2018) The determination of the efficacy of some microbial preparations against apple scab disease (Venturia inequalis (cke) wint.) in Isparta. BSJ Agric 1(1):1–8

  • Capdeville G, Beer SV, Watkins CB, Wilson CL, Tedeschi LO, Aist JR (2003) Pre- and post-harvest harpin protein treatments of apples ınduce resistance to blue mold. Plant Dis 87:39–44

    Article  Google Scholar 

  • Cheng W (2009) Rhizosphere priming effect: Its functional relationships with microbial turnover, evapotranspiration, and C- –N budgets. Soil Biol Biochem 41:1795–1801

    Article  Google Scholar 

  • Choi MS, Kim W, Lee C, Oh CS (2013) Harpins, multifunctional proteins secreted by gram-negative plant-pathogenic bacteria. MPMI 26(10):1115–1122. https://doi.org/10.1094/MPMI-02-13-0050-CR

    Article  Google Scholar 

  • Dervis S, Mercado-Blanco J, Erten L, Valverde-Corredor A, Pérez-Artés E (2010) Verticillium wilt of olive in Turkey: A survey on disease importance, pathogen diversity and susceptibility of relevant olive cultivars. Eur J Plant Pathol 127:287–301

    Article  Google Scholar 

  • Erkılıç A, Özdemir S, Akgül S (2018) Zeytinde Verticillium dahliae’ye karşı bazı dayanıklılık teşvik edici kimyasalların etkilerinin belirlenmesi. Çukurova Tarım Gıda Bilim Dergisi 33(1):69–76

    Google Scholar 

  • Erten L, Yıldız M (2004) Susceptibility of some economical important olive cultivars and clones to Verticillium dahliae Kleb in Turkey. Acta Hortic 791(2):559–565

    Google Scholar 

  • Ferguson F, Vossen PM, Teviotdale B (2008) UC IPM pest management guidelines: olive diseases. UC ANR Publication 3452. http://www.ipm.ucdavis.edu/PMG/r583100111.htm

  • Filho CJA, Pascholati SF, Sabrinho RR (2016) Mycorrhizal association and their role in plant disease protection. Plant, Soil and Microbes pp 95-143

    Chapter  Google Scholar 

  • Ghanbari R, Anwar F, Alkharfy KM, Gilani AH, Saari N (2012) Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.). A review. Int J Mol Sci 13(3):3291–3340

    Article  Google Scholar 

  • Gooch E (2005) Ten plus one things you may not know about olive. Epikouria Magazine

  • Haapalainen M, Engelhardt S, Kufner I, Li CM, Nurnberger T, Lee J (2011) Functional mapping of harpin HrpZ of Pseudomonas syringae reveals the sites responsible for protein oligomerization, lipid interactions and plant defence induction Mol. Plant Pathol 12:151–166

    Google Scholar 

  • Hanson LE (2000) Reduction of Verticillium wilt symptoms in cotton following seed treatment with Trichoderma virens. J Cotton Sci 4:224–231

    Google Scholar 

  • Harman GE (2006) Overview of mechanisms and uses of Trichoderma spp. Phytopathology 96:190–194

    Article  Google Scholar 

  • Hoitink HAJ, Madden LV, Dorrance AE (2006) Systemic resistance induced by Trichoderma spp.: interactions between the host, the pathogen, The biocontrol agent and soil organic matter quality. Phytopathology 90:186–189

    Article  Google Scholar 

  • Howell CR (2003) Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Dis 87(1):4–10

    Article  Google Scholar 

  • Jiménez-Díaz RM, Tjamos EC, Cirulli M (1998) Verticillium wilt of major tree hosts. Olive. In: Hiemstra JA, Harris DC (eds) A compendium of Verticillium wilts in tree species. Ponsen and Looijen, Wageningen, pp 13–16

    Google Scholar 

  • López-Escudero FJ, Blanco-López MA (2001) Effect of a single or double soil solarization to control Verticillium wilt in established olive orchards in Spain. Plant Dis 85:489–496

    Article  Google Scholar 

  • López-Escudero FJ, Blanco-López MA (2005) Isolation an morphologic characterization of microsclerotia of Verticillium dahliae isolates from soil. Biotech 4(4):296–304

    Article  Google Scholar 

  • Mechri B, Manga AGB, Tekaya M, Attia F, Cheheb H, Meriem FB, Braham M, Boujnah D, Hammami M (2014) Changes in microbial communities and carbohydrate profiles induced by the mycorrhizal fungus (Glomus intraradices) in rhizosphere of olive trees (Olea europaea L.). Appl Soil Ecol 75:124–133

    Article  Google Scholar 

  • Mishra S, Arora NK (2012) Evaluation of rhizospheric Pseudomonas and Bacillus as biocontrol tool for Xanthomonas campestris pv. campestris. World J Microbiol Biotechnol 28:693–702

    Article  Google Scholar 

  • Mishra J, Tewari S, Singh S, Arora NK (2015) Biopesticides: Where we stand? In: Arora NK (ed) Plant Microbes Symbiosis: Applied Facets. Springer, India, pp 37–76

    Google Scholar 

  • Nihorimbere V, Ongena M, Smargiassi M, Thonart P (2011) Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnol Agron Soc Environ 2:327–337

    Google Scholar 

  • Obradovic A, Jones JB, Momol TM, Balogh B, Olson SM (2004) Management of tomato bacterial spot in the field by foliar applications of bacteriophages and SAR ınducers. Plant Dis 88:736–740

    Article  Google Scholar 

  • Obradovic A, Jones JB, Momol MT, Olson SM, Jackson LE, Balogh B, Guven K, Iriarte FB (2005) Integration of biological control agents and systemic acquired resistance inducers against bacterial spot on tomato. Plant Dis 89:712–716

    Article  Google Scholar 

  • Onoğur E, Yolageldi L, Tunç C, Yıldırım İ (2001). Batı Anadolu zeytin ağaçlarında solgunluk hastalığının yaygınlığı ve etmeninin saptanması. Proje Raporu, Ege Ü. Z. F. Bitki Koruma Bölümü Bornova

  • Ryan D, Robards K (1998) Phenolic compounds in olives. Analyst 123:31R–44R

    Article  Google Scholar 

  • Saydam G, Copcu M (1972) Verticillium wilt of olive in Turkey. J Turk Phytopathol 1:45–49

    Google Scholar 

  • Silva K, Rebouças T, Pereira M, Silva D, São A, Sabin C (2008) Atividade antagônica in vitro de isolados de Trichoderma spp. ao fungo Phytophthora citrophthora. Agron 29(4):749–754

    Google Scholar 

  • Silva JAT, de Medeiros EV, da Silva JM, Tenorio DD, Moreira KA, Nascimento TCED (2016) Trichoderma aureoviride URM 5158 and Trichoderma hamatum URM 6656 are biocontrol agents that act against cassava root rot through different mechanisms. J Phytopathol 164:1003–1011. https://doi.org/10.1111/jph.12521

    Article  Google Scholar 

  • Tjamos EC (1993) Prospects and strategies in controlling Verticillium wilt of olive. Bull OEEP/EPPO Bull 23:505–512

    Article  Google Scholar 

  • Tjamos EC, Biris DA, Paplomatas EJ (1991) Recovery of olive trees from Verticillium wilt after individual application of soil solarisation in established olive orchards. Plant Dis 75:557–562

    Article  Google Scholar 

  • Townsend GK, Heuberger JW (1943) Methods for estimating losses caused by diseases in fungicide experiments. Plant Dis Rep 27:340–343

    Google Scholar 

  • Tubajika KM, Civerolo EL, Puterka GJ, Hashim JM, Luvisi DA (2006) The effects of kaolin, harpin protein and ımidacloropid on development of Pierce’s disease in grape. Crop Prot 26:92–99

    Article  Google Scholar 

  • TUIK (2017) Turkish Statistical Institute, http://www.tuik.gov.tr

  • Tunc C, Onogur E, Irshad M (2004) Investigations on alternative control measures of Verticillium wilt of olive. J Turk Phytopath 3(1-3):1–9

    Google Scholar 

  • Yıldız A, Benlioğlu S (2008) Trichoderma. harzianum’un pamuklarda çökerten (rhizoctonia solani Kühn) ve verticillium solgunluğu hastalığı (Verticillium dahliae Kleb.)’na etkisinin in-vivo koşullarda saptanması. ADÜ Ziraat Fakültesi Dergisi 6(1):3–7

    Google Scholar 

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Acknowledgements

This study was supported by the Süleyman Demirel University Research Fund, Project No. 4521-YL-15.

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  1. Agricultural Faculty, Department of Plant Protection, Isparta University of Applied Sciences, Isparta, 32260, Turkey

    Serife Evrim Arici & Ata Evren Demirtas

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  1. Serife Evrim Arici
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This article is part of the Topical Collection on <ital>Geo-Resources-Earth-Environmental Sciences</ital&gt.

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Arici, S.E., Demirtas, A.E. The effectiveness of rhizosphere microorganisms to control Verticillium wilt disease caused by Verticillium dahliae Kleb. in olives. Arab J Geosci 12, 781 (2019). https://doi.org/10.1007/s12517-019-4962-3

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