Abstract
Due to the recognized harmful effects of some trace elements on fetal development after long-term exposures, it is first important to address their basic/physiological levels before monitoring toxicological effects and clinical outcomes on prenatal and postnatal health. This research aimed to define, for the first time, reference values for multiple (ultra) trace elements in umbilical cord blood (UCB) plasma samples of a notable number of healthy pregnant women (n = 125). All samples were collected during 2020–2021 and all participants were from Belgrade and two regions (Šumadija and Podunavlje). Following trace elements were enrolled in this study: essential (Mn, Co, Cu, Zn, Se, Mo), toxic (Be, Al, Ni, As, Rb, Sr, Ag, Cd, Sb, Ba, Tl, Pb, Th, U), rare earth (La, Ce, Pr, Nd, Sm, Eu, Gd, Dy, Ho, Er, Tm), and noble metals (Ru, Rh, Pd, Re, Ir, Os, Pt). UCB plasma samples were diluted with suitable solvents and analyzed by inductively coupled plasma-mass spectrometry. The analytical technique used was validated by the standard addition method and using certified reference materials. Zn was found in the highest amount and Tm in the least amount. Be, Co, Ag, Sb, La, and Ce exhibited statistically significant differences in the four age groups (20 to 41 years), whereas Ag and Ce showed a tendency to increase with age. Furthermore, our participants had notably higher As, Ni, and Co, levels and lower Zn levels compared to other populations. This is the first study that, in addition to analyzing essential and toxic trace elements, also provided an analysis of noble and rare earth elements in UCB plasma samples. Presented results can be used as a starting point or database for further studies, in terms of predicting the pregnancy outcome and postnatal development.
Similar content being viewed by others
Data Availability
Data and Material are available from the corresponding author on reasonable request.
Code Availability
Not applicable.
References
Adabavazeh Z, Hwang W, Su Y (2017) Effect of adding cerium on microstructure and morphology of Ce-based inclusions formed in low-carbon steel. Sci Rep 7:46503. https://doi.org/10.1038/srep46503
Ahmed S, Mahabbat-e Khoda S, Rekha RS, Gardner RM, Ameer SS, Moore S, Ekström EC, Vahter M, Raqib R (2011) Arsenic-associated oxidative stress, inflammation, and immune disruption in human placenta and cord blood. Environ Health Perspect 119:258–264. https://doi.org/10.1289/ehp.1002086
Al-Saleh I, Shinwari N, Mashhour A, Rabah A (2014) Birth outcome measures and maternal exposure to heavy metals (lead, cadmium and mercury) in Saudi Arabian population. Int J Hyg Environ Health 217:205–218. https://doi.org/10.1016/j.ijheh.2013.04.009
Anual ZF, Mohammad Sham N, Ambak R et al (2021) Urinary concentrations of metals and metalloids in Malaysian adults. Expo Health 13:391–401. https://doi.org/10.1007/s12403-021-00390-z
Basta M, Lipsett BJ (2021) Anatomy, abdomen and pelvis, umbilical cord. StatPearls. StatPearls Publishing, Treasure Island
Bermúdez L, García-Vicent C, López J, Torró MI, Lurbe E (2015) Assessment of ten trace elements in umbilical cord blood and maternal blood: association with birth weight. J Transl Med 13:291. https://doi.org/10.1186/s12967-015-0654-2
Bocca B, Ruggieri F, Pino A, Rovira J, Calamandrei G, Martínez MÁ, Domingo JL, Alimonti A, Schuhmacher M (2019) Human biomonitoring to evaluate exposure to toxic and essential trace elements during pregnancy. Part A. Concentrations in maternal blood, urine and cord blood. Environ Res 177:108599. https://doi.org/10.1016/j.envres.2019.108599
Bocca B, Ruggieri F, Pino A, Rovira J, Calamandrei G, Mirabella F, Martínez MÁ, Domingo JL, Alimonti A, Schuhmacher M (2020) Human biomonitoring to evaluate exposure to toxic and essential trace elements during pregnancy. Part B: predictors of exposure. Environ Res 182:109108. https://doi.org/10.1016/j.envres.2019.109108
Burtis CA, Ashwood ER, Bruns DE, Tietz NW (2012) Textbook of clinical chemistry and molecular diagnostics, 5th edn. Elsevier, Amsterdam
Butler Walker J, Houseman J, Seddon L, McMullen E, Tofflemire K, Mills C, Corriveau A, Weber JP, LeBlanc A, Walker M, Donaldson SG, Van Oostdam J (2006) Maternal and umbilical cord blood levels of mercury, lead, cadmium, and essential trace elements in Arctic Canada. Environ Res 100:295–318. https://doi.org/10.1016/j.envres.2005.05.006
Cabral-Pinto MMS, Inácio M, Neves O et al (2020) Human health risk assessment due to agricultural activities and crop consumption in the surroundings of an industrial area. Expo Health 12:629–640. https://doi.org/10.1007/s12403-019-00323-x
Cardenas A, Koestler DC, Houseman EA, Jackson BP, Kile ML, Karagas MR, Marsit CJ (2015) Differential DNA methylation in umbilical cord blood of infants exposed to mercury and arsenic in utero. Epigenetics 10:508–515. https://doi.org/10.1080/15592294.2015.1046026
Chandyo RK, Ulak M, Kvestad I, Shrestha M, Ranjitkar S, Basnet S, Hysing M, Shrestha L, Strand TA (2017) The effects of vitamin B12 supplementation in pregnancy and postpartum on growth and neurodevelopment in early childhood: study protocol for a randomized placebo controlled trial. BMJ Open 7:e016434. https://doi.org/10.1136/bmjopen-2017-016434
Dursun A, Yurdakok K, Yalcin SS, Tekinalp G, Aykut O, Orhan G, Morgil GK (2016) Maternal risk factors associated with lead, mercury and cadmium levels in umbilical cord blood, breast milk and newborn hair. J Matern Fetal Neonatal Med 29:954–961. https://doi.org/10.3109/14767058.2015.1026255
Dutta S, Gorain B, Choudhury H, Roychoudhury S, Sengupta P (2021) Environmental and occupational exposure of metals and female reproductive health. Environ Sci Pollut Res Int 24:1–26. https://doi.org/10.1007/s11356-021-16581-9
Ellingsen DG, Moller LB, Aaseth J (2005) Copper. In: Nordberg F, Fowler BA, Nordberg M (eds) Handbook on the toxicology of metals, 4th edn. Academic Press, San Diego, pp 765–786
Emsley AM, Stevens GC (2008) The risks and benefits of flame retardants in consumer products. Woodhead Publishing, Sawston, pp 363–397. https://doi.org/10.1533/9781845694701.3.363
Ettinger AS, Arbuckle TE, Fisher M, Liang CL, Davis K, Cirtiu CM, Bélanger P, LeBlanc A, Fraser WD, MIREC Study Group (2017) Arsenic levels among pregnant women and newborns in Canada: results from the maternal-infant research on environmental chemicals (MIREC) cohort. Environ Res 153:8–16. https://doi.org/10.1016/j.envres.2016.11.008
Hooper SB, Binder-Heschl C, Polglase GR et al (2016) The timing of umbilical cord clamping at birth: physiological considerations. Matern Health Neonatol Perinatol 2:4. https://doi.org/10.1186/s40748-016-0032-y
Iwai-Shimada M, Kameo S, Nakai K, Yaginuma-Sakurai K, Tatsuta N, Kurokawa N, Nakayama SF, Satoh H (2019) Exposure profile of mercury, lead, cadmium, arsenic, antimony, copper, selenium and zinc in maternal blood, cord blood and placenta: the Tohoku study of child development in Japan. Environ Health Prev Med 17:24–35. https://doi.org/10.1186/s12199-019-0783-y
Jagodić J, Rovčanin B, Borković-Mitić S, Lj V, Avdin V, Manojlović D, Stojsavljević A (2021) Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues. Environ Sci Pollut Res Int 24:1–8. https://doi.org/10.1007/s11356-021-14013-2
Jariwala M, Suvarna S, Kiran Kumar G, Amin A, Udas AC (2014) Study of the concentration of trace elements Fe, Zn, Cu, Se and their correlation in maternal serum, cord serum and colostrums. Indian J Clin Biochem 29:181–188. https://doi.org/10.1007/s12291-013-0338-8
Jeevanaraj P, Hashim Z, Elias SM et al (2019) Risk of dietary mercury exposure via marine fish ingestion: assessment among potential mothers in Malaysia. Expo Health 11:227–236. https://doi.org/10.1007/s12403-017-0270-x
Kot K, Kosik-Bogacka D, Łanocha-Arendarczyk N, Malinowski W, Szymański S, Mularczyk M, Tomska N, Rotter I (2019) Interactions between 14 elements in the human placenta, fetal membrane and umbilical cord. Int J Environ Res Public Health 16:1615. https://doi.org/10.3390/ijerph16091615
Lee MS, Eum KD, Golam M, Quamruzzaman Q, Kile ML, Mazumdar M, Christiani DC (2021) Umbilical cord blood metal mixtures and birth size in Bangladeshi children. Environ Health Perspect 129:57006. https://doi.org/10.1289/EHP7502
Leung M, Nøst TH, Wania F et al (2020) Maternal-child exposures to persistent organic pollutants in Dhaka, Bangladesh. Expo Health 12:79–87. https://doi.org/10.1007/s12403-018-0286-x
Li A, Zhuang T, Shi J, Liang Y, Song M (2019) Heavy metals in maternal and cord blood in Beijing and their efficiency of placental transfer. J Environ Sci (china) 80:99–106. https://doi.org/10.1016/j.jes.2018.11.004
Liang C, Li Z, Xia X, Wang Q, Tao R, Tao Y, Xiang H, Tong S, Tao F (2017) Determine multiple elements simultaneously in the sera of umbilical cord blood samples-a very simple method. Biol Trace Elem Res 177:1–8. https://doi.org/10.1007/s12011-016-0853-6
Liang C, Wang J, Xia X, Wang Q, Li Z, Tao R, Tao Y, Xiang H, Tong S, Tao F (2018) Serum cobalt status during pregnancy and the risks of pregnancy-induced hypertension syndrome: a prospective birth cohort study. J Trace Elem Med Biol 46:39–45. https://doi.org/10.1016/j.jtemb.2017.11.009
Liang CM, Wu XY, Huang K, Yan SQ, Li ZJ, Xia X, Pan WJ, Sheng J, Tao YR, Xiang HY, Hao JH, Wang QN, Tao FB, Tong SL (2019) Trace element profiles in pregnant women’s sera and umbilical cord sera and influencing factors: repeated measurements. Chemosphere 218:869–878. https://doi.org/10.1016/j.chemosphere.2018.11.115
Lukasiewicz M, Czerniecki J, Ponikwicka-Tyszko D et al (2021) Placenta is capable of protecting the male fetus from exposure to environmental bisphenol A. Expo Health 13:1–14. https://doi.org/10.1007/s12403-020-00358-5
Maksimović ZJ, Djujić I, Jović V, Ršumović M (1992) Selenium deficiency in Yugoslavia. Biol Trace Elem Res 33:187–196
McKeating DR, Fisher JJ, Zhang P, Bennett WW, Perkins AV (2020) Elemental metabolomics in human cord blood: method validation and trace element quantification. J Trace Elem Med Biol 59:126419. https://doi.org/10.1016/j.jtemb.2019.126419
Osada H, Watanabe Y, Nishimura Y, Yukawa M, Seki K, Sekiya S (2002) Profile of trace element concentrations in the feto-placental unit in relation to fetal growth. Acta Obstet Gynecol Scand 81:931–937. https://doi.org/10.1034/j.1600-0412.2002.811006.x
Parajuli RP, Fujiwara T, Umezaki M, Furusawa H, Ser PH, Watanabe C (2012) Cord blood levels of toxic and essential trace elements and their determinants in the Terai region of Nepal: a birth cohort study. Biol Trace Elem Res 147:75–83. https://doi.org/10.1007/s12011-011-9309-1
Rădulescu A, Lundgren S (2019) A pharmacokinetic model of lead absorption and calcium competitive dynamics. Sci Rep 9:14225. https://doi.org/10.1038/s41598-019-50654-7
Reddy YS, Aparna Y, Ramalaksmi BA, Kumar BD (2014) Lead and trace element levels in placenta, maternal and cord blood: a cross-sectional pilot study. J Obstet Gynaecol Res 40:2184–2190. https://doi.org/10.1111/jog.12469
Rudge CV, Röllin HB, Nogueira CM, Thomassen Y, Rudge MC, Odland JØ (2009) The placenta as a barrier for toxic and essential elements in paired maternal and cord blood samples of South African delivering women. J Environ Monit 11:1322–1330. https://doi.org/10.1039/b903805a
Sakamoto M, Yasutake A, Domingo JL, Chan HM, Kubota M, Murata K (2013) Relationships between trace element concentrations in chorionic tissue of placenta and umbilical cord tissue: potential use as indicators for prenatal exposure. Environ Int 60:106–111. https://doi.org/10.1016/j.envint.2013.08.007
Sakamoto M, Chan HM, Domingo JL, Koriyama C, Murata K (2018) Placental transfer and levels of mercury, selenium, vitamin E, and docosahexaenoic acid in maternal and umbilical cord blood. Environ Int 111:309–315. https://doi.org/10.1016/j.envint.2017.11.001
Shulman HB, D’Angelo DV, Harrison L, Smith RA, Warner L (2018) The pregnancy risk assessment monitoring system (PRAMS): overview of design and methodology. Am J Public Health 108:1305–1313. https://doi.org/10.2105/AJPH.2018.304563
Silver MK, Arain AL, Shao J, Chen M, Xia Y, Lozoff B, Meeker JD (2018) Distribution and predictors of 20 toxic and essential metals in the umbilical cord blood of Chinese newborns. Chemosphere 210:1167–1175. https://doi.org/10.1016/j.chemosphere.2018.07.124
Stojsavljević A, Trifković J, Rasić-Milutinović Z, Jovanović D, Bogdanović G, Mutić J, Manojlović D (2018) Determination of toxic and essential trace elements in serum of healthy and hypothyroid respondents by ICP-MS: a chemometric approach for discrimination of hypothyroidism. J Trace Elem Med Biol 48:134–140. https://doi.org/10.1016/j.jtemb.2018.03.020
Stojsavljević A, Jagodić J, Lj V, Borković-Mitić S, Rašić-Milutinović Z, Jovanović D, Gavrović-Jankulović M, Manojlović D (2020) Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency. Environ Sci Pollut Res Int 27:1397–1405. https://doi.org/10.1007/s11356-019-06936-8
Stojsavljević A, Rovčanin M, Rovčanin B, Miković Ž, Jeremić A, Perović M, Manojlović D (2021) Human biomonitoring of essential, nonessential, rare earth, and noble elements in placental tissues. Chemosphere 285:131518. https://doi.org/10.1016/j.chemosphere.2021.131518
Taylor TP, Ding M, Ehler DS, Foreman TM, Kaszuba JP, Sauer NN (2003) Beryllium in the environment: a review. J Environ Sci Health A 38:439–469. https://doi.org/10.1081/ESE-120016906
Wani AL, Ara A, Usmani JA (2015) Lead toxicity: a review. Interdiscip Toxicol 8:55–64. https://doi.org/10.1515/intox-2015-0009
Zhou C, Zhang R, Cai X, Xiao R, Yu H (2019) Trace elements profiles of maternal blood, umbilical cord blood, and placenta in Beijing, China. J Matern Fetal Neonatal Med 32:1755–1761. https://doi.org/10.1080/14767058.2017.1416602
Acknowledgements
This research was financially supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Contract Number: 451-03-68/2020-14/200288.
Author information
Authors and Affiliations
Contributions
AS designed the research, conducted the experimental study, analyzed the data, and wrote the first and last versions of the paper; MR collected clinical specimens; MR, JJ, ŽM, AJ, MP, and DM participated in writing through reviewing and editing. All authors contributed critically to the draft and gave final approval for publication.
Corresponding author
Ethics declarations
Conflict of interest
The authors report no conflicts of interest.
Ethical Approval
This investigation was approved by the Institutional Ethical Review Board (No: 05006-2021-1525) from Belgrade, Serbia.
Consent to Participate
All patients and healthy blood donors voluntarily participated in this study, and written informed consent from all study participants was obtained according to the ethical standards defined by the Declaration of Helsinki.
Consent for Publication
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Stojsavljević, A., Rovčanin, M., Jagodić, J. et al. Evaluation of Maternal Exposure to Multiple Trace Elements and Their Detection in Umbilical Cord Blood. Expo Health 14, 623–633 (2022). https://doi.org/10.1007/s12403-021-00441-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12403-021-00441-5