Abstract
One of the objectives of microbiological water quality studies is to identify and minimise public health risks from exposure to bathing waters.
Water-related diseases, particularly those caused by viral pathogens, are still of major concern. Gastrointestinal symptoms experienced by bathers following immersion in surface waters are mainly due to viral, rather than bacterial, infection.
However, there does not appear to be a correlation between the number of pathogenic viruses present in the water matrices and Escherichia coli, the parameter most frequently used to establish microbial water quality.
This study describes the spatial and temporal distribution of somatic coliphages compared with conventional bacterial indicators (E. coli and Enterococci) in the waters off San Cataldo (South Adriatic Sea, Lecce—Italy), a beach resort near the city of Lecce subject to high anthropogenic impact.
Our results indicate that somatic coliphages are less sensitive to environmental factors (temperature and dilution) than the classical indicators of faecal contamination.
These findings suggest that somatic coliphages could be used as potential indicators for evaluating the quality of coastal seawaters and treated wastewaters discharged into the sea, in order to provide a more accurate analysis of the hygienic-sanitary quality of this water matrix.
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References
Allwood PB, Malik YS, Maherchandani S, Hedberg CW, Goyal SM (2005) Effect of temperature on the survival of F-specific RNA coliphage, Feline calcinvirus and Escherichia coli in chlorinated water. Int J Environ Res Public Health 2(3):442–446
American Public Health Association (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington. Part 9000
Berkelman R, King G, Gerba C, Haas C, Rose J, Sobsey M (2004) Water quality criteria for bacteria for coastal and Great Lakes waters. American Society for Microbiology, Washington. (Online) Posting date 4 August 2004. http://www.asm.org/Policy/index.asp?bid_29781
Carducci A, Cantiani L, Ruschi MA (1994) A contribution to the standardization of methods for the concentration of enterovirus from seawater. Ig Mod 45:707–721
Council Directive 76/160/EEC Concerning the quality of bathing water. 8 December 1975. Official Journal of the European Communities No. L 31, 5 February 1976
Council Directive 91/271/EEC Concerning urban waste water treatment, 21 May 1991. Official Journal of the European Communities, No. L 135, 30 May 1991, pp 40–52
Council Directive 91/676/EEC Concerning the protection of waters against pollution caused by nitrates from agricultural sources, 12 December 1991. Official Journal of the European Communities, no. L 375, 31 December 1991. Correction of mistakes included in the DOCE No. L 92, of 16 April 1993
Council Directive 2000/60/EC of the European Parliament and of the Council of the 23 October 2000 establishing a framework for Community action in the field of water policy. Official Journal No L 327, 22 December 2000
Council Directive 2006/7/EC of the European Parliament and of the Council of 15 February 2006 concerning the management of bathing water quality and repealing Directive 76/160/EEC Official Journal L 064, 04/03/2006 pp 0037–0051
De Donno A, Bagordo F, Liaci D, Lugoli F, Gabutti G (2008a) Mytilus galloprovincialis (Lam.) as a bioindicator of microbiological pollution of coastal waters. A study conducted in the Salento peninsula (Italy). J Coast Res 24(1):216–221
De Donno A, Bagordo F, Lugoli F, Leopizzi MI, Fanizzi FP (2008b) Dynamics of microbial autochthon populations and human impact on the harbour of Otranto (Lecce, Italy). J Coast Res 24(3):103–109
Dizer H, Wolf S, Fischer M, López-Pila JM, Röske I, Schmidt R, Szewzyk R, Wiedenmann A (2005) Die novelle der EU-badegewässerrichtlinie. Aspekte der risikobewertung bei der Grenzwertsetzung. Bundesgesundheitsbl-Gesundheitsforsch-Gesundheitsschutz 48:607–614 (The EU bathing water directive. Risk assessment and standards)
Donia D, Divizia M, Panà A (1998) Analysis of concentration methods for bacteriophages. Ig Mod 109:475–482
EN ISO 10705-1 (1995) Water quality: detection and enumeration of bacteriophages. Part 1: enumeration of F-specific RNA bacteriophages
EN ISO 10705-2 (2000a) Water quality: detection and enumeration of bacteriophages. Part 2: enumeration of somatic coliphages
EN ISO 9308-1 (2000b) Water quality—Detection and enumeration of Escherichia coli and coliform bacteria—Part 1: Membrane filtration method
EN ISO 10705-4 (2001) Water quality: detection and enumeration of bacteriophages. Part 4: enumeration of bacteriophages infecting Bacteroides fragilis
Environment Agency (2004) Bathing water Environment Agency Assessment. London DEFRA. Available from http://www.defra.gov.uk/environment/water/quality/bathing/pdf/bw_ea_assess.pdf. Accessed 4 December 2004
Gabutti G, De Donno A, Erroi R, Liaci D, Montagna MT (2004) Relationship between indicators of faecal pollution and presence of pathogenic microorganisms in coastal seawater. J Coast Res 20:846–852
Gantzer C, Henny J, Schwartzbrod L (2002) Bacteroides fragilis and Escherichia coli bacteriophages in human faeces. Int J Hyg Environ Health 205:325–328
Garin D, Fuchs F, Crance JM, Rouby Y, Chapalain J, Lamarque D, Gounot AM, Aymard M (1994) Exposure to enteroviruses and Hepatitis A virus among divers in environmental waters in France: first biological ans seroloical survey of a controlled cohort. Epidemiol Infect 113:541–549
Gassilloud B, Schwartzbrod L, Gantzer C (2003) Presence of viral genomes in mineral water: A sufficient condition to assume infectious risk? Appl Environ Microbiol 69:3965–3969
Grabow WOK (2001) Bacteriophages: Update on application as models for viruses in water. Water SA 27:251–268
Grassi T, Bagordo F, Idolo A, Lugoli F, Gabutti G, De Donno A (2009) Rotavirus detection in environmental water samples by tangential flow ultrafiltration and RT-nested PCR. Environ Monit Assess. doi:10.1007/s10661-009-0885-x
Gratacap-Cavalier B, Genoulaz O, Brengel-Pesce K (2000) Detection of human and animal Rotavirus sequences in drinking water. Appl Environ Microbiol 66:2690–2692
Harwood VJ, Levine AD, Scott TM, Chivukula V, Lukasik J, Farrah SR, Rose JB (2005) Validity of the indicator organism paradigm for pathogen reduction in reclaimed water and public health protection. Appl Environ Microbiol 71:3163–3170
IAWPRC Study Group on Health Related Water Microbiology (1991) Bacteriophages as model viruses in water quality control. Water Res 25:529–545
Ibarluzea JM, Marina LS, Moreno B, Serrano E, Larburu K, Maiztegi MJ, Yarzabal A (2007) Somatic coliphages and bacterial indicators of bathing water quality in the beaches of Gipuzkoa, Spain. J Water Health 5(3):417–426
Melnick JL (1984) Enteric viruses in water. Monograf Virol 15:1–16
Mocé-Llivina L, Lucena F, Jofre J (2005) Enteroviruses and Bacteriophages in bathing waters. Appl Environ Microbiol 71(11):6838–6844
Muniesa M, Jofre J (2007) The contribution of induction of temperate phages to the numbers of free somatic coliphages in waters is not significant. FEMS Microbiol Lett 270:272–276
Myrmel M, Rimstad, E.,Berg EMM, Grinde B (2006) Enteric viruses in inlet and outlet samples from sewage treatment plants. J Water Health 197–209
Oshima KH (2001) Efficient and predictable recovery of viruses and Cryptosporium Parvum oocysts from water by ultrafiltration system. New Mexico: Water Resources Research Institute, Technical Completion Report 316, 1–55
Palmateer GA, Dutka BJ, Janzen EM, Meissner SM, Sakellaris MG (1991) Coliphages and bacteriophages as indicators of recreational water quality. Water Res 25:355–357
Payment P (1998) Waterborne viruses and parasites: resistance to treatment and disinfection. OECD Workshop Molecular Methods for Safe Drinking Water Interlaken
Ricca DM, Cooney JJ (1999) Coliphages and indicator bacteria in Boston Harbor, Massachusetts. Environ Toxicol Water Qual 14(4):404–408
Scientific Committee on Toxicity, Ecotoxicity, and Environment. (2004) Bathing water quality. European Economic Commission, Brussels, Belgium. http://www.europa.eu.int/water/water-bathing/index_en.html
Serrano E, Moreno B et al. (1998) The influence of environmental factors on microbiological indicators of coastal water pollution. Water Sci Technol 38(12):195–199
Skraber S, Gantzer C, Maul A, Schwartzbrod L (2002) Fates of bacteriophages and bacterial indicators in the Moselle River (France). Water Res 36:3629–3637
Vantarakis AC, Tsibouxi A, Venieri D, Komninou G, Papapetropoulou M, Athanassiadou A (2005) Evaluation of microbiological quality of coastal waters in Greece. J Water Health 03:371–380
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Lugoli, F., Leopizzi, M.I. & De Donno, A. Viral Indicators of Hygienic-Sanitary Quality: Detection of Somatic Coliphages in the Southern Adriatic Sea. Water Expo. Health 1, 171–177 (2009). https://doi.org/10.1007/s12403-009-0016-5
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DOI: https://doi.org/10.1007/s12403-009-0016-5