The effects of different dense phase carbon dioxide (DPCD) treatments were evaluated on technological parameters, physicochemical and textural properties and microbiological quality of lamb sausages. Sausages were prepared using lamb meat (Longissimus dorsii, 68.6 % w/w), lamb fat (17.2 % w/w), water (12.9 % w/w) and NaCl (1.37 % w/w). Raw sausages were subjected to different CO2 pressures (10, 20 and 30 MPa) at 60 °C–30 min and treatment times (2, 14 and 25 min) at 55 °C–10 MPa. Weight loss, pH and total expressible fluid increased significantly (p < 0.05) as pressure increased from 0 to 30 MPa. These parameters also increased significantly (p < 0.05) as treatment time increased (at 10 MPa). The increase in CO2 pressure and treatment time significantly (p < 0.05) modified internal and surface color parameters. Lightness and b* values increased, whereas redness (a*) decreased. Also, the increase in CO2 pressure and treatment time significantly (p < 0.05) increased Warner–Bratzler shear force and textural parameters values. DPCD treatments may modify meat proteins, which may lead to weak interactions among proteins and formation of gel-like structures. Regarding microbial inactivation, the highest reductions in microbiological counts (2 Log CFU g−1) were obtained applying a CO2 pressure of 20 MPa at 60 °C for 30 min.
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Acton JC, Dawson PL (2004) Chapter 25: impact of proteins in food colour. In: Yada RY (ed) Proteins in food processing. Woodhead Publishing Limited & CRC Press, London, pp 631–668
Chao RR, Mulvaney SJ, Bailey ME, Fernando LN (1991) Supercritical CO2 conditions affecting extraction of lipid and cholesterol from ground beef. J Food Sci 56:183–187. doi:10.1111/j.1365-2621.1991.tb08007.x
Choi YM, Bae YY, Kim KH, Kim BC, Rhee MS (2009) Effects of supercritical carbon dioxide treatment against generic Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, and E. coli O157:H7 in marinades and marinated pork. Meat Sci 82:419–424. doi:10.1016/j.meatsci.2009.02.016
Choi YM, Kim OY, Kim KH, Kim BC, Rhee MS (2009) Combined effect of organic acids and supercritical carbon dioxide treatments against nonpathogenic Escherichia coli, Listeria monocytogenes, Salmonella typhimurium and E. coli O157:H7 in fresh pork. Lett Appl Microbiol 49:510–515. doi:10.1111/j.1472-765X.2009.02702.x
Choi YM et al (2008) Effects of supercritical carbon dioxide treatment for sterilization purpose on meat quality of porcine longissimus dorsi muscle. Food Sci Technol 41:317–322. doi:10.1016/j.lwt.2007.02.020
Damar S, Balaban MO (2006) Review of dense phase CO2 technology: microbial and enzyme inactivation, and effects on food quality. J Food Sci 71:R1–R11. doi:10.1111/j.1365-2621.2006.tb12397.x
Debs-Louka E, Louka N, Abraham G, Chabot V, Allaf K (1999) Effect of compressed carbon dioxide on microbial cell viability. Appl Environ Microbiol 65:626–631
Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW (2011) InfoStat, versión 24-03-2011 edn. Universidad Nacional de Córdoba, Córdoba
Ferrentino G, Balzan S, Spilimbergo S (2013) Optimization of supercritical carbon dioxide treatment for the inactivation of the natural microbial flora in cubed cooked ham. Int J Food Microbiol 161:189–196. doi:10.1016/j.ijfoodmicro.2012.12.004
Ferrentino G, Balzan S, Spilimbergo S (2013) Supercritical carbon dioxide processing of dry cured ham spiked with Listeria monocytogenes: inactivation kinetics, color, and sensory evaluations. Food Bioprocess Technol 6:1164–1174. doi:10.1007/s11947-012-0819-4
Ferrentino G, Spilimbergo S (2011) High pressure carbon dioxide pasteurization of solid foods: current knowledge and future outlooks. Trends Food Sci Technol 22:427–441. doi:10.1016/j.tifs.2011.04.009
Garcia-Gonzalez L et al (2007) High pressure carbon dioxide inactivation of microorganisms in foods: the past, the present and the future. Int J Food Microbiol 117:1–28. doi:10.1016/j.ijfoodmicro.2007.02.018
Jakobsen M, Bertelsen G (2002) The use of CO2 in packaging of fresh red meats and its effects on chemical quality changes in the meat: a review. J Muscle Foods 13:143–168. doi:10.1111/j.1745-4573.2002.tb00326.x
Ledward DA (1979) Meat. In: Priestley RJ (ed) Effect of heating on food-stuffs. Applied Science Publishers, London, pp 121–157
Lee CM, Whiting RC, Jenkins RK (1987) Texture and sensory evaluations of frankfurters made with different formulations and processes. J Food Sci 52:896–900. doi:10.1111/j.1365-2621.1987.tb14237.x
Lin H-M, Cao N, Chen L-F (1994) Antimicrobial effect of pressurized carbon dioxide on Listeria monocytogenes. J Food Sci 59:657–659. doi:10.1111/j.1365-2621.1994.tb05587.x
Messens W, Van Camp J, Huyghebaert A (1997) The use of high pressure to modify the functionality of food proteins. Trends Food Sci Technol 8:107–112. doi:10.1016/s0924-2244(97)01015-7
Qu YL, Zhang DQ, Rao WL, Li CH, Li J (2010) Influence of dense phase CO2 on gel properties of minced mutton. J Nucl Agric Sci 24:1226–1231
Sirisee U, Hsieh F, Huff HE (1998) Microbial safety of supercritical carbon dioxide processes. J Food Process Preserv 22:387–403. doi:10.1111/j.1745-4549.1998.tb00358.x
Szerman N (2009) Estudio de la incorporación de aditivos de origen natural a músculos bovinos sometidos a cocción-pasteurización mediante el sistema sous vide. PhD, Universidad Nacional de la Plata
Szerman N, Gonzalez CB, Sancho AM, Grigioni G, Carduza F, Vaudagna SR (2012) Effect of the addition of conventional additives and whey proteins concentrates on technological parameters, physicochemical properties, microstructure and sensory attributes of sous vide cooked beef muscles. Meat Sci 90:701–710
Yan W, Cui J, Dai R, Wang H, Li X (2010) Effects of dense phase carbon dioxide on quality and physical-chemical properties of chilled pork. Trans CSAE 26:346–350
This work was funded by the Argentinean-China Cooperation Project: “Safety improvement and shelf life extension of fresh and cooked beef and mutton products applying non-thermal technologies,” Argentina-China Bi-National Center for Food Science and Technology and by the Project 31171774 “Proteins and their structure changes related to Escherichia coli death after dense phase carbon dioxide treatment,” National Natural Science Foundation of China.
Natalia Szerman and Wei Li Rao have contributed equally to this work and should be considered co-first authors.
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Szerman, N., Rao, W.L., Li, X. et al. Effects of the Application of Dense Phase Carbon Dioxide Treatments on Technological Parameters, Physicochemical and Textural Properties and Microbiological Quality of Lamb Sausages. Food Eng Rev 7, 241–249 (2015). https://doi.org/10.1007/s12393-014-9092-9
- Sausage quality
- Lamb meat