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Formation of Protein Bodies and the Response to Nitrogen in Different Positions During Wheat Endosperm Development

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Abstract

The development of protein bodies (PBs) determines the processing properties of wheat (Triticum aestivum L.). It has been known that nitrogen uptake has a strong impact on grain protein concentration. However, the differences of the formation of PB in different developmental stages and different positions in wheat endosperm are still controversial. To solve these issues, PBs formation in different wheat endosperm parts and the response to nitrogen were investigated using light, transmission electron microscopes in present study. The main results were as follows. (1) Proteins mainly formed PBs via the Golgi apparatus in the vacuole at the early stage of wheat endosperm development. After 7 days post anthesis, most proteins were derived from the rough endoplasmic reticulum. (2) The morphology of PBs was diverse among different endosperm parts. Compared with the central endosperm, the PBs in sub-aleurone cells were abundant and large. (3) More abundant endoplasmic reticulum, Golgi and mitochondrion were observed at the early stage after nitrogen treatment. Nitrogen also increased the accumulation of PBs at the later stage. (4) The sub-aleurone region cells of the endosperm presented more significant responses to nitrogen than the central endosperm.

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Acknowledgements

The research was supported by the Natural Science Foundation (31571573, 31701351), a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops/Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou, 225009, China

    Yang Yang, Xinyu Chen, Liping Ran, Yunfei Wu, Xurun Yu, Zhaodi Dong & Fei Xiong

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  1. Yang Yang
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  2. Xinyu Chen
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  3. Liping Ran
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  4. Yunfei Wu
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  7. Fei Xiong
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Correspondence to Fei Xiong.

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Yang, Y., Chen, X., Ran, L. et al. Formation of Protein Bodies and the Response to Nitrogen in Different Positions During Wheat Endosperm Development. J. Plant Biol. 62, 274–285 (2019). https://doi.org/10.1007/s12374-019-0051-0

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