Overexpression of ICE1, a Regulator of Cold-Induced Transcriptome, Confers Cold Tolerance to Transgenic Zoysia japonica
- 120 Downloads
Numerous genes are known to regulate the cold response in plants. The ICE1 gene is a regulator of coldinduced transcriptome playing an important role in cold tolerance in Arabidopsis thaliana. Here, the Arabidopsis ICE1 gene was cloned and transformed into A. thaliana and Korean lawn grass (Zoysia japonica) respectively, under the control of a maize ubiquitin promoter. The transgenic Arabidopsis plants showed higher survival rate, less MDA content, and higher levels of proline, SOD, and POD after freezing treatment. In the case of Z. japonica, 43 transgenic plants were produced by Agrobacterium-mediated transformation. Southern blot analysis showed that 9 transgenic lines selected by phosphinothricin (PPT) were transformed with ICE1 independently. Cold treatment significantly induced the transcripts abundant in the up-regulated ZjCBF and ZjDREB1 in transgenic lines OE-18 and OE-19. Meanwhile, transgenic plants showed increased proline level but decreased MDA content and with higher activities of SOD and POD compared with wild type under cold stress. These results suggest that overexpression of the ICE1 gene could improve the antioxidative capacity in conferring cold tolerance to the transgenic zoysiagrass plants.
KeywordsCold tolerance ICE1 Overexpression Zoysia japonica
Unable to display preview. Download preview PDF.
- Beard JB (1973) Turfgrass: science and culture. Englewood Cliffs: Prentice Hall pp 261Google Scholar
- Chinnusamy V, Zhu JK, Sunkar R (2010) Gene regulation during cold stress acclimation in plants. Methods Mol Biol 639:9–55Google Scholar
- Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59:309–314Google Scholar
- Hyun YH, Choi BJ, Kim YJ, Joo YK (2012) Analysis of research trend on zoysiagrass (Zoysia spp.). Asian J Turfgrass Sci 26:89–95Google Scholar
- Li S, Yang Y, Zhang Q, Liu N, Xu Q, Hu L (2018) Differential physiological and metabolic response to low temperature in two zoysiagrass genotypes native to high and low latitude. PloS One 13:e0198885Google Scholar
- Liu W, Yu K, He T, Li F, Zhang D, Liu J (2013) The low temperature induced physiological responses of Avena nuda L., a coldtolerant plant species. Sci World J 2013:658793Google Scholar
- Sharma NYS (2016) Reactive Oxygen Species, Oxidative stress and ROS scavenging system in plants. J Chem Pharm Res 8:595–604Google Scholar
- Toyama K, Bae CH, Kang JG, Lim YP, Adachi T, Riu KZ, Song PS, Lee HY (2003) Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation. Mol Cells 16:19–27Google Scholar
- Wei S, Du Z, Gao F, Ke X, Li J, Liu J, Zhou Y (2015) Global transcriptome profiles of'Meyer'Zoysiagrass in response to cold stress. PLoS One 10:e0131153Google Scholar