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Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus

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Abstract

Plants are promising host systems for recombinant protein production. However, progress in the commercialization of plant-made proteins (PMPs) has been slow. Only one PMP drug is commercially available. In this study, we explored the possibility of using plants to produce E2 of classical swine fever virus (CSFV) and the use of this plant-produced E2 as a vaccine. We designed high-level expression vectors for transgenic plants by considering the transcription, translation, and storage of E2 in the cell. We incorporated a cellulosebinding domain sequence into the expression vector as an affinity tag for cost-effective, one-step purification. Using this vector, we generated multiple lines of transgenic Arabidopsis thaliana plants expressing a fusion protein of E2 from CSFV at high levels (0.7% of total soluble proteins). ER-targeted E2 fusion protein was successfully purified via a one-step purification process using amorphous cellulose resin. Arabidopsis-produced E2 was recognized by an antibody that detects CSFV antigen. Finally, antisera from mice immunized with E2 fusion protein reacted strongly to the antigens in a CSFV antibody detection kit. Therefore, we propose that plant-produced E2 fusion proteins could be further developed for use as a green vaccine against CSFV in animals.

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Authors and Affiliations

  1. BioApplications Inc., Pohang Techno Park Complex, 394 Jigok-ro Nam-gu, Pohang, 37668, Korea

    Eun-Ju Sohn, Yongjik Lee, Namjo Park, Minhee Park, Nam Hyung Kim, Soohong Park, Kyungmin Min, Sungmin Gu & Youngmin Park

  2. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 37673, Korea

    Eun-Ju Sohn, Yongjik Lee, Youngmin Park & Inhwan Hwang

  3. Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, Korea

    Inhwan Hwang

  4. Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongbuk, 39660, Korea

    Jaeyoung Song & Dong-jun An

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  1. Eun-Ju Sohn
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  2. Yongjik Lee
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  3. Namjo Park
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  4. Minhee Park
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Correspondence to Inhwan Hwang.

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Sohn, EJ., Lee, Y., Park, N. et al. Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus. J. Plant Biol. 61, 241–252 (2018). https://doi.org/10.1007/s12374-018-0133-4

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