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Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature

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Abstract

Thermosensitive male sterility plays an important role in wheat fertility and production. As a key enzyme for chlorophyll degradation, pheophorbide a oxygenase (PaO) can suppress cell death in plants. We cloned the wheat gene TaPaO1 from the thermosensitive genetic male sterile (TGMS) line BS366; it encodes a typical PaO protein, containing a conserved Rieske [2Fe-2S] iron–sulphur motif, a mononuclear non-heme iron-binding motif, and a C-terminal CxxC motif. TaPaO1 was expressed in all tissues and was upregulated during the meiosis stage of BS366 anthers under low temperature. Subcellular localization of TaPaO1 specifically labelled the surrounding of chloroplasts. TaPaO1 regulated by RD29A promoter which responded to low temperature led to pollen sterility in transgenic tobacco. Expression analysis showed that TaPaO1 exhibited a higher level of expression in the anther than in other tissues in transgenic tobacco plants during low temperature treatment. We propose that the higher senescence-related activity of TaPaO1 may lead to the cell death of anthers, which happens at an early developmental stage under low temperature. These results provide new insights into the function of PaO during the early developmental stage of anthers. PaO is closely related to cell death regardless of whether it exhibits increased activity or inactive.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry, Beijing, 100097, China

    Guoliang Yuan, Yukun Wang, Shaohua Yuan, Peng Wang, Wenjing Duan, Jianfang Bai, Hui Sun, Na Wang, Fengting Zhang, Liping Zhang & Changping Zhao

  2. The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat, Beijing, 100097, China

    Guoliang Yuan, Yukun Wang, Shaohua Yuan, Peng Wang, Wenjing Duan, Jianfang Bai, Hui Sun, Na Wang, Fengting Zhang, Liping Zhang & Changping Zhao

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  1. Guoliang Yuan
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  2. Yukun Wang
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  3. Shaohua Yuan
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  4. Peng Wang
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  5. Wenjing Duan
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  6. Jianfang Bai
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  7. Hui Sun
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  8. Na Wang
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  9. Fengting Zhang
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  10. Liping Zhang
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  11. Changping Zhao
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Correspondence to Liping Zhang or Changping Zhao.

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Yuan, G., Wang, Y., Yuan, S. et al. Functional Analysis of Wheat TaPaO1 Gene Conferring Pollen Sterility Under Low Temperature. J. Plant Biol. 61, 25–32 (2018). https://doi.org/10.1007/s12374-017-0269-7

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  • DOI: https://doi.org/10.1007/s12374-017-0269-7

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