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Diverse chromosomal rearrangements induced by Ac/Ds at the OsRLG5 locus in regenerated rice plants

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Abstract

Closely-located transposable elements (TEs) can induce chromosomal breakage and rearrangements via alternative transposition (AT) reactions. Our previous study showed that diverse chromosomal rearrangements were induced by two inversely-oriented Ds elements at the OsRLG5 (Oryza sativa receptor-like kinase gene 5) locus in regenerated rice plants. Here, several novel chromosomal rearrangements were identified in a population of tissue culture regenerants, and their proposed Ac/Ds-transposition mechanisms were discussed. In the first type of event, AT was induced by the same Ds element on each of two sister chromatids and resulted in the inversion of genomic DNA flanking the Ds element. In the second type of event, DNA segments of the inter-transposon segment (ITS) region were deleted or inverted. This novel local rearrangement likely resulted from an AT reaction of two directly-oriented Ds elements located on the same chromatid. The 5' and 3' termini of two Ds elements were re-inserted into two different sites inside the ITS, which generated the subsequent deletion and inversion. In the third type of event, two inversely-oriented Ds elements induced homologous recombination and chromosomal translocation. Together, these results showed the diversity of Ac/Ds-mediated chromosomal rearrangements and demonstrated the utility of the experimental system for examining the mechanisms underlying Ac/Ds AT reactions.

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References

  • Chin HG, Choe MS, Lee SH, Park SH, Park SH, Koo JC, Kim NY, Lee JJ, Oh BG, Yi GH, Kim SC, Choi HC, Cho MJ, Han CD (1999) Molecular analysis of rice plants harboring an Ac/Ds transposable element-mediated gene trapping system. Plant J 19:615–624

    Article  CAS  PubMed  Google Scholar 

  • Dooner HK, Belachew A (1991) Chromosome breakage by pairs of closely linked transposable elements of the Ac-Ds family in maize. Genetics 129:855–862

    PubMed Central  CAS  PubMed  Google Scholar 

  • English JJ, Harrison K, Jones J (1995) Aberrant transpositions of maize Double Ds-like elements usually involve Ds ends on sister chromatids. Plant Cell 7:1235–1247

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hirochika H, Sugimoto K, Otsuki Y, Tsugawa H, Kanda M (1996) Retrotransposons of rice involved in mutations induced by tissue culture. Proc Natl Acad Sci USA 93:7783–7788

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hirochika H (1997) Retrotransposons of rice: their regulation and use for genome analysis. Plant Mol Biol 35:231240

    Article  Google Scholar 

  • Huang JT, Dooner HK (2008) Macrotrasposition and other complex chromosomal restructuring in maize by closely linked transposons in direct orientation. Plant Cell 20:2019–2032

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kim CM, Je BI, Piao HL, Park SJ, Kim MJ, Park SH, Park JY, Park SH, Lee EK, Chon NS, Won YJ, Lee GH, Nam MH, Yun DW, Lee MC, Cha YS, Lee KH, Eun MY, Han CD (2002) Reprogramming of the activity of the Activator/Dissociation transposon family during plant regeneration in rice. Mol Cells 14:231–237

    CAS  PubMed  Google Scholar 

  • Kim CM, Piao HL, Park SJ, Chon NS, Je BI, Sun B, Park SH, Park JY, Lee EJ, Kim MJ, Chung WS, Lee KH, Lee YS, Lee JJ, Won YJ, Yi G, Nam MH, Cha YS, Yun DW, Eun MY, Han CD (2004) Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice. Plant J 39:252–263

    Article  CAS  PubMed  Google Scholar 

  • Krishnaswamy L, Zhang J, Peterson T (2008) Reversed end Ds element: a novel tool for chromosome engineering in Arabidopsis. Plant Mol Biol 68:399–411

    Article  CAS  PubMed  Google Scholar 

  • McClintock B (1984) The significance of responses to the genome to challenge. Science 226:792–801

    Article  CAS  PubMed  Google Scholar 

  • Page DR, Kohler C, Da Costa-Nunes JA, Baroux C, Moore JM, Grossniklaus U (2004) Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis. Proc Natl Acad Sci USA 101:2969–2974

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Park SJ, Piao HL, Xuan YH, Park SH, Je BI, Kim CM, Lee EJ, Park SH, Ryu BC, Lee KH, Lee GH, Nam MH, Yeo US, Lee MC, Yun DW, Eun MY, Han CD (2006) Analysis of intragenic Ds transpositions and excision events generating novel allelic variation in rice. Mol Cells 21:284–293

    CAS  PubMed  Google Scholar 

  • Peschke VM, Phillips RL, Gengenbach BG (1987) Discovery of transposable element activity among progeny of tissue culturederived maize plants. Science 238:804–807

    Article  CAS  PubMed  Google Scholar 

  • Ralston EJ, English J, Dooner,HK (1989) Chromosome-breaking structure in maize involving a fractured Ac element. Proc Natl Acad Sci USA 86:9451–9455

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Weil CF, Wessler SR (1993) Molecular evidence that chromosome breakage by Ds elements is caused by aberrant transposition. Plant Cell 5:515–522

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Xuan YH, Piao HL, Je BI, Park SJ, Park SH, Huang J, Zhang JB, Peterson T, Han CD. (2011) Transposon Ac/Ds-induced chromosomal rearrangements at the rice OsRLG5 locus. Nucleic Acids Res 39:e149

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yu C, Zhang J, Pulletikuri V, Weber DF, Peterson, T (2010) Spatial configuration of transposable element Ac termini affects their ability to induce chromosomal breakage in maize. Plant Cell 22:744–754

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yu C, Han F, Zhang J, Birchler J, Peterson T (2012) A transgenic system for generation of transposon Ac/Ds-induced chromosome rearrangements in rice. Theor Appl Genet 125:1449–1462

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang J, Peterson T (1999) Genome rearrangements by nonlinear transposons in maize. Genetics 153:1403–1410

    PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang J, Peterson T (2005) A segmental deletion series generated by sister-chromatid transposition of Ac transposable elements in maize. Genetics 171:333–344

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang J, Peterson T (2004) Transposition of reversed Ac element ends generates chromosome rearrangements in maize. Genetics 167:1929–1937

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang J, Zhang F, Peterson T (2006) Transposition of reversed Ac element ends generates novel chimeric genes in maize. PLoS Genet 6:2(10) e164

    Article  Google Scholar 

  • Zhang J, Yu C, Pulletikuri V, Lamb J, Danilova T, Weber DF, Birchler J, Peterson T (2009) Alternative Ac/Ds transposition induces major chromosomal rearrangements in maize. Genes Dev 23:755–765

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang J, Zuo T, Peterson T (2013) Generation of tandem direct duplications by reversed-ends transposition of maize AC elements. PLoS Genet 9:e1003691

    Article  PubMed Central  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. College of Plant Protection, Shenyang Agricultural University, Dongling Road 120, Shenyang, Liaoning, 110866, China

    Yuan Hu Xuan

  2. Department of biological science, Wonkwang University, Iksan, Jeonbuk, 570-749, Korea

    Soon Ju Park

  3. Department of Southern Area Crop Science, NICS (National Institute of Crop Science), RDA, 20th Jeompiljaero, Miryang, Gyeongnam, 627-803, Korea

    Jun Hyeon Cho

  4. Rural Development Administration, National Academy of Agricultural Science, Suwon, 441-857, Korea

    Tae Ho Kim

  5. Division of Applied Life Science (BK21 Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, 660-701, Korea

    Yuan Hu Xuan & Chang-deok Han

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  1. Yuan Hu Xuan
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  2. Soon Ju Park
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  3. Jun Hyeon Cho
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  4. Tae Ho Kim
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  5. Chang-deok Han
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Correspondence to Chang-deok Han.

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Xuan, Y.H., Park, S.J., Cho, J.H. et al. Diverse chromosomal rearrangements induced by Ac/Ds at the OsRLG5 locus in regenerated rice plants. J. Plant Biol. 58, 318–326 (2015). https://doi.org/10.1007/s12374-015-0270-y

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  • DOI: https://doi.org/10.1007/s12374-015-0270-y

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